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Literature summary for 3.6.1.11 extracted from

  • Lichko, L.P.; Kulakovskaya, T.V.; Kulakovskaya, E.V.; Kulaev, I.S.
    Inactivation of PPX1 and PPN1 genes encoding exopolyphosphatases of Saccharomyces cerevisiae does not prevent utilization of polyphosphates as phosphate reserve (2008), Biochemistry (Moscow), 73, 985-989.
    View publication on PubMed
Search for more literature for 3.6.1.11

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Saccharomyces cerevisiae
heparin
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cell envelope
-
 Saccharomyces cerevisiae 30313
-
cytosol
-
 Saccharomyces cerevisiae 5829
-
cytosol appears in the cytosol under PPX1 inactivation Saccharomyces cerevisiae 5829
-
mitochondrial membrane
-
 Saccharomyces cerevisiae 31966
-
mitochondrion soluble fraction Saccharomyces cerevisiae 5739
-
nucleus
-
 Saccharomyces cerevisiae 5634
-
vacuole
-
 Saccharomyces cerevisiae 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 determined by gel filtration Saccharomyces cerevisiae
450000
-
 determined by gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae PPN1 splitting long polyphosphate chains to shorter ones ?
-
 ?
additional information Saccharomyces cerevisiae PPX1 splitting off phosphate from the end of the polyphosphate chain ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 polyphosphate hydrolysis in the CRX strain cytosol completes in 120 min Saccharomyces cerevisiae
0.006
-
 strain CRX with inactivated ppx1 and ppN1 gene, cytosol preparation in exponential growth phase in phosphate-deficient medium Saccharomyces cerevisiae
0.01
-
 strain CRX with inactivated ppx1 and ppN1 gene, cytosol preparation in exponential growth phase in phosphate-rich medium Saccharomyces cerevisiae
0.04
-
 strain CRX with inactivated ppx1 gene, cytosol preparation in exponential growth phase in phosphate-rich medium Saccharomyces cerevisiae
0.055
-
 strain CRX with inactivated ppx1 gene, cytosol preparation in exponential growth phase in phosphate-deficient medium Saccharomyces cerevisiae
0.07
-
 strain CRX with inactivated ppn1 gene, cytosol preparation in exponential growth phase in phosphate-rich medium Saccharomyces cerevisiae
0.095
-
 strain CRX with inactivated ppn1 gene, cytosol preparation in exponential growth phase in phosphate-deficient medium Saccharomyces cerevisiae
0.1
-
 parent strain CRY, cytosol preparation in exponential growth phase in phosphate-deficient medium Saccharomyces cerevisiae
0.18
-
 parent strain CRY, cytosol preparation in exponential growth phase in phosphate-rich medium Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PPN1 splitting long polyphosphate chains to shorter ones Saccharomyces cerevisiae ?
-
 ?
additional information PPX1 splitting off phosphate from the end of the polyphosphate chain Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
exopolyphosphatase
-
 Saccharomyces cerevisiae
Ppn1
-
 Saccharomyces cerevisiae
PPX1
-
 Saccharomyces cerevisiae