Cloned (Comment) | Organism |
---|---|
gene PPX1, overexpression | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
enzxme in complex with phosphate, sulfate, or ATP, X-ray diffraction structure determination and analysis at 1.6, 1.8, and 1.9 A resolution, multiple isomorphous replacement with anomalous scattering technique | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45058 | - |
x * 45058, recombinant enzyme, mass spectrometry | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)n + H2O | Saccharomyces cerevisiae | - |
(phosphate)n-1 + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
gene PPX1 | - |
Purification (Comment) | Organism |
---|---|
recombinant PPX1 17.4fold in a two-step procedure | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2070 | - |
purified recombinant enzyme | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)n + H2O | - |
Saccharomyces cerevisiae | (phosphate)n-1 + phosphate | - |
? | |
(phosphate)n + H2O | the cytosolic exopolyphosphatase that processively cleaves the terminal phosphate group from the polyphosphate chain, until inorganic diphosphate is all that remains, structure of the substrate binding channel, overview | Saccharomyces cerevisiae | (phosphate)n-1 + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45058, recombinant enzyme, mass spectrometry | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
More | PPX1 belongs to the DHH family of phosphoesterases | Saccharomyces cerevisiae |
PPX1 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1550 | - |
(Phosphate)n | 37°C, recombinant enzyme | Saccharomyces cerevisiae |