Literature summary for 3.6.1.11 extracted from

Ugochukwu, E.; Lovering, A.L.; Mather, O.C.; Young, T.W.; White, S.A.
The crystal structure of the cytosolic exopolyphosphatase from Saccharomyces cerevisiae reveals the basis for substrate specificity (2007), J. Mol. Biol., 371, 1007-1021.

Search for more literature for 3.6.1.11

Cloned(Commentary)

Cloned (Comment)	Organism
gene PPX1, overexpression	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzxme in complex with phosphate, sulfate, or ATP, X-ray diffraction structure determination and analysis at 1.6, 1.8, and 1.9 A resolution, multiple isomorphous replacement with anomalous scattering technique	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Saccharomyces cerevisiae	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45058	-	x * 45058, recombinant enzyme, mass spectrometry	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(phosphate)n + H2O	Saccharomyces cerevisiae	-	(phosphate)n-1 + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	gene PPX1	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant PPX1 17.4fold in a two-step procedure	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2070	-	purified recombinant enzyme	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(phosphate)n + H2O	-	Saccharomyces cerevisiae	(phosphate)n-1 + phosphate	-	?
(phosphate)n + H2O	the cytosolic exopolyphosphatase that processively cleaves the terminal phosphate group from the polyphosphate chain, until inorganic diphosphate is all that remains, structure of the substrate binding channel, overview	Saccharomyces cerevisiae	(phosphate)n-1 + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 45058, recombinant enzyme, mass spectrometry	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
More	PPX1 belongs to the DHH family of phosphoesterases	Saccharomyces cerevisiae
PPX1	-	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1550	-	(Phosphate)n	37°C, recombinant enzyme	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)