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Literature summary for 3.6.1.11 extracted from

  • Rodrigues, C.O.; Ruiz, F.A.; Vieira, M.; Hill, J.E.; Docampo, R.
    An acidocalcisomal exopolyphosphatase from Leishmania major with high affinity for short chain polyphosphate (2002), J. Biol. Chem., 277, 50899-50906.
    View publication on PubMed
Search for more literature for 3.6.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli of a his-tagged recombinant enzyme Leishmania major

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibitor Leishmania major
heparin 80% inhibition at 0.1 mM Leishmania major
Zn2+ strong inhibitor Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
26.8
-
 (Polyphosphate)n n = 4, pH 7.2 Leishmania major
28.1
-
 (Polyphosphate)n n = 3, pH 7.2 Leishmania major
39.1
-
 (Polyphosphate)n n = 15, pH 7.2 Leishmania major
54.5
-
 guanosine 5'-tetraphosphate pH 7.2 Leishmania major
57.6
-
 (Polyphosphate)n n = 75, pH 7.2 Leishmania major
1343
-
 (Polyphosphate)n n = 45, pH 7.2 Leishmania major

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Leishmania major 5829
-
vacuole
-
 Leishmania major 5773
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ little effect on polyP15 hydrolysis Leishmania major
Mg2+ best activator at 1 mM using polyP3, polyP4 and polyP15 as substrates Leishmania major
Mn2+ best activator for guanosine 5'-tetraphosphate hydrolysis Leishmania major
additional information no activity measured in absence of divalent cations Leishmania major

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, SDS-PAGE Leishmania major

Organism

Organism UniProt Comment Textmining
Leishmania major
-
 promastigotes
-

Purification (Commentary)

Purification (Comment) Organism
isolation of a recombinant His-tagged protein by affinity chromatography followed by cleavage of the polyhistidine tag Leishmania major

Storage Stability

Storage Stability Organism
-80°C, 300 mM NaCl, 250 mM sucrose, 2 mM dithiothreitiol, several months, no activity loss Leishmania major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(polyphosphate)n + H2O no acyivity if n = 200 Leishmania major (polyphosphate)n-1 + phosphate
-
 ?
guanosine 5'-tetraphosphate + H2O
-
 Leishmania major ?
-
 ?
additional information ATP, diphosphate and p-nitrophenyl phosphate are not substrates Leishmania major ?
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Leishmania major

Synonyms

Synonyms Comment Organism
LmPPX
-
 Leishmania major

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 hydrolysis of polyP3 Leishmania major
7 8
-
 Leishmania major