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Literature summary for 3.6.1.11 extracted from
- An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon (1993), J. Biol. Chem., 268, 633-639.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | 50 mM, stimulates | Escherichia coli |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | - |
Escherichia coli | |
| Polyphosphate | polyP15, 50% inhibition at 200fold polymer molar excess over the long-chain polyphosphate substrate | Escherichia coli | |
| Tetrapolyphosphate | 50% inhibition at 2000fold polymer molar excess over the long-chain polyP substrate | Escherichia coli | |
| tripolyphosphate | weak inhibition at 2000fold polymer molar excess over the long-chain polyP substrate | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 175 mM, stimulates | Escherichia coli | |
| Mg2+ | 1 mM, required | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
2 * 57000, SDS-PAGE | Escherichia coli |
| 58133 | - |
2 * 58133, calculation from nucleotide sequence | Escherichia coli |
| 100000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K 12 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (polyphosphate)n + H2O | n = 500 | Escherichia coli | (polyphosphate)n-1 + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 57000, SDS-PAGE | Escherichia coli |
| dimer | 2 * 58133, calculation from nucleotide sequence | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
about | Escherichia coli |
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