Any feedback?
Literature summary for 3.6.1.1 extracted from
- Structure of inorganic pyrophosphatase from Staphylococcus aureus reveals conformational flexibility of the active site (2015), J. Struct. Biol., 189, 81-86.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| isoform PpaC in complex with Mn2+, hanging drop vapor diffusion method, using 1.32 M ammonium citrate tribasic | Staphylococcus aureus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R295A | inactive | Staphylococcus aureus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Staphylococcus aureus | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | dependent on, the active site contains two catalytic Mn2+ binding sites | Staphylococcus aureus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
2 * 34000, SDS-PAGE | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | Staphylococcus aureus | - |
2 phosphate | - |
? | |
| diphosphate + H2O | Staphylococcus aureus Mu50 | - |
2 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | P65752 | - |
- |
| Staphylococcus aureus Mu50 | P65752 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-IMAC HisTrap column chromatography and S-200 gel filtration | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | - |
Staphylococcus aureus | 2 phosphate | - |
? | |
| diphosphate + H2O | - |
Staphylococcus aureus Mu50 | 2 phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 34000, SDS-PAGE | Staphylococcus aureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| inorganic pyrophosphatase | - |
Staphylococcus aureus |
| PpaC | - |
Staphylococcus aureus |
| PPIase | - |
Staphylococcus aureus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
