Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 cells | Thermococcus thioreducens |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 25% (w/v) 2-methyl-2,4-pentanediol, 100 mM sodium acetate pH 4.6, 10 mM calcium chloride, 2 mM ammonium sulfate | Thermococcus thioreducens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20850 | - |
x * 20850, calculated from amino acid sequence | Thermococcus thioreducens |
21000 | - |
x * 21000, SDS-PAGE | Thermococcus thioreducens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Thermococcus thioreducens | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus thioreducens | H2L2L6 | - |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography and Sephadex S-200 gel filtration | Thermococcus thioreducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Thermococcus thioreducens | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 21000, SDS-PAGE | Thermococcus thioreducens |
? | x * 20850, calculated from amino acid sequence | Thermococcus thioreducens |
Synonyms | Comment | Organism |
---|---|---|
inorganic pyrophosphatase | - |
Thermococcus thioreducens |
IPPase | - |
Thermococcus thioreducens |