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Literature summary for 3.6.1.1 extracted from

  • May, A.; Berger, S.; Hertel, T.; Koeck, M.
    The Arabidopsis thaliana phosphate starvation responsive gene AtPPsPase1 encodes a novel type of inorganic pyrophosphatase (2011), Biochim. Biophys. Acta, 1810, 178-185.
    View publication on PubMed
Search for more literature for 3.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
At1g73010, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain Bl21 (DE3) Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
D19A site-directed mutagenesis Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0388
-
 diphosphate pH 8.0, 37°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol soluble cytoplasmic enzyme Arabidopsis thaliana 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates to 50% of the activity with Mg2+ at 2 mM Arabidopsis thaliana
Fe2+ activates to 88% of the activity with Mg2+ at 2 mM Arabidopsis thaliana
Mg2+ dependent on, 10 mM Arabidopsis thaliana
Mn2+ activates to 39% of the activity with Mg2+ at 2 mM Arabidopsis thaliana
Ni2+ activates to 83% of the activity with Mg2+ at 2 mM Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33494
-
 4 * 33500, SDS-PAGE, 4 * 33494, sequence calculation Arabidopsis thaliana
33500
-
 4 * 33500, SDS-PAGE, 4 * 33494, sequence calculation Arabidopsis thaliana
120000
-
 recombinant wild-type enzyme, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Arabidopsis thaliana
-
 2 phosphate
-
 ?
diphosphate + H2O Arabidopsis thaliana Col-0
-
 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q67YC0
-
-
Arabidopsis thaliana Col-0 Q67YC0
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the enzyme shares the catalytic mechanism of the HAD superfamily including a phosphorylated enzyme intermediate Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Arabidopsis thaliana

Reaction

Reaction Comment Organism Reaction ID
diphosphate + H2O = 2 phosphate the catalytic mechanism of the HAD superfamily including a phosphorylated enzyme intermediate, overview Arabidopsis thaliana
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00705
-
 purified recombinant wild-type enzyme, pH 8.0, 37°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phosphorylethanolamine + H2O low activity Arabidopsis thaliana ethanolamine + phosphate
-
 ?
4-nitrophenyl phosphate + H2O low activity Arabidopsis thaliana 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O low activity Arabidopsis thaliana Col-0 4-nitrophenol + phosphate
-
 ?
D-glucose-6-phosphate + H2O low activity Arabidopsis thaliana D-glucose + phosphate
-
 ?
diphosphate + H2O
-
 Arabidopsis thaliana 2 phosphate
-
 ?
diphosphate + H2O AtPPsPase1 catalyzes the specific cleavage Arabidopsis thaliana 2 phosphate
-
 ?
diphosphate + H2O
-
 Arabidopsis thaliana Col-0 2 phosphate
-
 ?
diphosphate + H2O AtPPsPase1 catalyzes the specific cleavage Arabidopsis thaliana Col-0 2 phosphate
-
 ?
additional information substrate specificity, overview. The enzyme is highly specific, no or poor activity with ribose-5-phosphate, phospho-L-serine, o-phosphocholine, phosphoenolpyruvate, L-glycerol-3-phosphate, beta-glycerol-phosphate, and phospho-L-tyrosine Arabidopsis thaliana ?
-
 ?
additional information substrate specificity, overview. The enzyme is highly specific, no or poor activity with ribose-5-phosphate, phospho-L-serine, o-phosphocholine, phosphoenolpyruvate, L-glycerol-3-phosphate, beta-glycerol-phosphate, and phospho-L-tyrosine Arabidopsis thaliana Col-0 ?
-
 ?
thiamine diphosphate + H2O low activity Arabidopsis thaliana ?
-
 ?
thiamine diphosphate + H2O low activity Arabidopsis thaliana Col-0 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 33500, SDS-PAGE, 4 * 33494, sequence calculation Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtPPsPase1
-
 Arabidopsis thaliana
inorganic pyrophosphatase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana sequence calculation
-
 5.2

Expression

Organism Comment Expression
Arabidopsis thaliana the enzyme is induced by phosphate starvation up

General Information

General Information Comment Organism
evolution AtPPsPase1 belongs to the haloacid dehalogenase, HAD, superfamily Arabidopsis thaliana
additional information modelling of the active site Arabidopsis thaliana
physiological function tight control of AtPPsPase1 gene expression underlines its important role in the phosphate starvation response, cleavage of diphosphate is an immediate metabolic adaptation reaction Arabidopsis thaliana