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Literature summary for 3.6.1.1 extracted from

  • Park, S.; Lee, B.; Park, K.; Chong, Y.; Yoon, M.; Jeon, S.; Kim, D.
    Facilitation of polymerase chain reaction with thermostable inorganic pyrophosphatase from hyperthermophilic archaeon Pyrococcus horikoshii (2010), Appl. Microbiol. Biotechnol., 85, 807-812.
    View publication on PubMed
Search for more literature for 3.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21 pLysS(DE3) Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Pyrococcus horikoshii
-
 2 phosphate
-
 ?
diphosphate + H2O Pyrococcus horikoshii DSM 12428
-
 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O59570
-
-
Pyrococcus horikoshii DSM 12428 O59570
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Pyrococcus horikoshii 2 phosphate
-
 ?
diphosphate + H2O development and evaluation of a phosphate-based colorimetric assay to measure progress of PCR using the PPase-coupled enzyme assay, overview Pyrococcus horikoshii 2 phosphate
-
 ?
diphosphate + H2O
-
 Pyrococcus horikoshii DSM 12428 2 phosphate
-
 ?
diphosphate + H2O development and evaluation of a phosphate-based colorimetric assay to measure progress of PCR using the PPase-coupled enzyme assay, overview Pyrococcus horikoshii DSM 12428 2 phosphate
-
 ?

Synonyms

Synonyms Comment Organism
inorganic pyrophosphatase
-
 Pyrococcus horikoshii
PPase
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pyrococcus horikoshii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 95 active at Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 95 recombinant PhPPase, robust catalytic activity Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pyrococcus horikoshii