Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme | Papaver rhoeas |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibits sPPase activity of the enzyme, 62% inhibition of p26.1a, 49% of p26.1b | Papaver rhoeas | |
additional information | the enzyme is inhibited by phosphorylation | Papaver rhoeas |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Papaver rhoeas | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on, cannot be substituted by Mn2+ or Ca2+ | Papaver rhoeas |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24400 | - |
x * 24400, p26.1a, SDS-PAGE, x * 26500, p26.1b, SDS-PAGE | Papaver rhoeas |
26500 | - |
x * 24400, p26.1a, SDS-PAGE, x * 26500, p26.1b, SDS-PAGE | Papaver rhoeas |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Papaver rhoeas | the enzyme is regulated by reversible phosphorylation, another mechanism in regulation of several physiological processes, e.g. self-incompatibility-mediated pollen tube inhibition, overview | 2 phosphate | - |
? | |
additional information | Papaver rhoeas | cytosolic phosphoproteins p26.1 from incompatible pollen show rapid, self-incompatibility-induced Ca2+-dependent hyperphosphorylation in vivo, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver rhoeas | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme is inhibited by in vitro phosphorylation, self-incompatibility induces Ca21-dependent phosphorylation of p26.1 | Papaver rhoeas |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme by nickel affinity chromatography | Papaver rhoeas |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pollen | hydrated, specific for, high activity | Papaver rhoeas | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2170 | - |
purified p26.1b | Papaver rhoeas |
2320 | - |
purified p26.1a | Papaver rhoeas |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Papaver rhoeas | 2 phosphate | - |
? | |
diphosphate + H2O | the enzyme is regulated by reversible phosphorylation, another mechanism in regulation of several physiological processes, e.g. self-incompatibility-mediated pollen tube inhibition, overview | Papaver rhoeas | 2 phosphate | - |
? | |
additional information | cytosolic phosphoproteins p26.1 from incompatible pollen show rapid, self-incompatibility-induced Ca2+-dependent hyperphosphorylation in vivo, overview | Papaver rhoeas | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24400, p26.1a, SDS-PAGE, x * 26500, p26.1b, SDS-PAGE | Papaver rhoeas |
Synonyms | Comment | Organism |
---|---|---|
inorganic pyrophosphatase | - |
Papaver rhoeas |
p26.1a | - |
Papaver rhoeas |
p26.1b | - |
Papaver rhoeas |
phosphoprotein p26.1 | - |
Papaver rhoeas |
sPPAse | - |
Papaver rhoeas |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Papaver rhoeas | p26.1b, sequence calculation | - |
6.03 |
Papaver rhoeas | p26.1a, sequence calculation | - |
6.11 |