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Literature summary for 3.6.1.1 extracted from

  • Jaemsen, J.; Tuominen, H.; Salminen, A.; Belogurov, G.A.; Magretova, N.N.; Baykov, A.A.; Lahti, R.
    A CBS domain-containing pyrophosphatase of Moorella thermoacetica is regulated by adenine nucleotides (2007), Biochem. J., 408, 327-333.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.1

Activating Compound

Activating Compound Comment Organism Structure
ATP 1.6fold activation at 0.1 mM Moorella thermoacetica

Cloned(Commentary)

Cloned (Comment) Organism
expression of the enzyme in Escherichia coli BL21(DE3) Moorella thermoacetica

Protein Variants

Protein Variants Comment Organism
Y169A site-directed mutagenesis of the CBS domain residue, the mutant shows altered kinetics with adenine nucleotides compared to the wild-type enzyme Moorella thermoacetica

Inhibitors

Inhibitors Comment Organism Structure
ADP complete inhibition at 0.1 mM Moorella thermoacetica
AMP 96% inhibition at 0.1 mM Moorella thermoacetica
cAMP 58% inhibition at 0.1 mM Moorella thermoacetica
CDP 50% inhibition at 0.1 mM Moorella thermoacetica
GDP 19% inhibition at 0.1 mM Moorella thermoacetica
GMP 18% inhibition at 0.1 mM Moorella thermoacetica
additional information poor inhibition by adenosine and UTP, no inhibition by cytidine, guanosine, uridine, CMP, CTP, GTP, cGMP, and UMP Moorella thermoacetica
UDP 35% inhibition at 0.1 mM Moorella thermoacetica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic analysis of wild-type and mutant enzymes in presence of different nucleotides, overview Moorella thermoacetica

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble CBS-PPase Moorella thermoacetica
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ CBS-PPase requires transition metal ions, Co2+ or Mn2+, for activity Moorella thermoacetica
Mn2+ CBS-PPase requires transition metal ions, Co2+ or Mn2+, for activity Moorella thermoacetica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Moorella thermoacetica
-
 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica
-
 strain ATCC 35608
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli BL21(DE3) by anion exchange chromatography and gel filtration Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Moorella thermoacetica 2 phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer the enzyme CBS-PPase contains cystathionine beta-synthase domains Moorella thermoacetica

Synonyms

Synonyms Comment Organism
CBS-PPase
-
 Moorella thermoacetica
family II PPase
-
 Moorella thermoacetica
inorganic pyrophosphatase
-
 Moorella thermoacetica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Moorella thermoacetica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.64
-
 diphosphate pH 7.2, 25°C Moorella thermoacetica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Moorella thermoacetica