Cloned (Comment) | Organism |
---|---|
expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3) | Streptococcus agalactiae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, 5-20 mg/ml protein in 20 mM Tris-HCl, pH 7.0, hanging drop vapour diffusion method, 0.001 ml of protein and well solution are mixed, 2 weeks at room temperature, X-ray diffraction structure determination and analysis at 2.8 A resolution | Streptococcus agalactiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | dependent on | Streptococcus agalactiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus agalactiae | Q3K0B5 | - |
- |
Streptococcus agalactiae A909 | Q3K0B5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography, removal of the tag by thrombin | Streptococcus agalactiae |
Synonyms | Comment | Organism |
---|---|---|
family II inorganic pyrophosphatase | - |
Streptococcus agalactiae |
manganese-dependent inorganic pyrophosphatase | - |
Streptococcus agalactiae |