Activating Compound | Comment | Organism | Structure |
---|---|---|---|
methylenebisphosphonate | activates the hexameric but not the dimeric form of the enzyme at pH 6 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E145Q | easy formation of dimers with 5% of activity of the corresponding hexamer, 5fold increase of Kd for Mg2+ at site 2, no inhibition with high concentrations of Mg2+ | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
diphosphate | wild type hexamer, pH 7.5 | Escherichia coli | |
0.8 | - |
diphosphate | wild type dimer, pH 7.5 | Escherichia coli | |
1.3 | - |
diphosphate | E145Q hexamer, pH 7.5 | Escherichia coli | |
1.8 | - |
diphosphate | E145Q dimer, pH 7.5 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, inhibitory for the native hexamer above 5 mM, no inhibition of the dimeric form | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Escherichia coli | important for energy metabolism, provides energy for biosynthetic reactions | 2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Escherichia coli | 2 phosphate | - |
? | |
diphosphate + H2O | important for energy metabolism, provides energy for biosynthetic reactions | Escherichia coli | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | an active dimer can be obtained using 20% isopropanol from the native hexamer | Escherichia coli |
hexamer | native form, composed of two trimers | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.7 | - |
diphosphate | E145Q mutant dimer, pH 7.5 | Escherichia coli | |
76 | - |
diphosphate | wild type dimer, pH 7.5 | Escherichia coli | |
83 | - |
diphosphate | E145Q mutant hexamer, pH 7.5 | Escherichia coli | |
390 | - |
diphosphate | wild type hexamer, pH 7.5 | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Escherichia coli |