KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
diphosphate | pH 6.5, wild type, hydrolysis of diphosphate | Escherichia coli | |
0.0018 | - |
diphosphate | pH 7.2, D97E, hydrolysis of diphosphate | Escherichia coli | |
0.0024 | - |
diphosphate | pH 8, D97E, hydrolysis of diphosphate | Escherichia coli | |
0.0034 | - |
diphosphate | pH 8, wild type, hydrolysis of diphosphate | Escherichia coli | |
0.0035 | - |
diphosphate | pH 7.2, wild type, hydrolysis of diphosphate | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | four Mg2+ ions per active site required for catalysis | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Escherichia coli | - |
2 phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Escherichia coli | 2 phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
diphosphate | pH 7.2, D97E, resynthesis of diphosphate | Escherichia coli | |
0.67 | - |
diphosphate | pH 8, D97E, resynthesis of diphosphate | Escherichia coli | |
12 | - |
diphosphate | pH 7.2, D97E, hydrolysis of diphosphate | Escherichia coli | |
55 | - |
diphosphate | pH 8, D97E, hydrolysis of diphosphate | Escherichia coli | |
86 | - |
diphosphate | pH 6.5, wild type, hydrolysis of diphosphate | Escherichia coli | |
104 | - |
diphosphate | pH 6.5, wild type, resynthesis of diphosphate | Escherichia coli | |
114 | - |
diphosphate | pH 8, wild type, resynthesis of diphosphate | Escherichia coli | |
116 | - |
diphosphate | pH 7.2, wild type, resynthesis of diphosphate | Escherichia coli | |
155 | - |
diphosphate | pH 7.2, wild type, hydrolysis of diphosphate | Escherichia coli | |
187 | - |
diphosphate | pH 8, wild type, hydrolysis of diphosphate | Escherichia coli |