Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | hydrolysis of imidophosphate competitively inhibited in the absence of divalent cations | Bos taurus | |
p-chloromercuriphenyl sulfonate | 50% inhibition of hydrolysis of diphosphate at 25 µM | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
Imidodiphosphate | - |
Bos taurus | |
0.14 | - |
diphosphate | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | increases hydrolysis of imidophosphate | Bos taurus | |
Mg2+ | increases hydrolysis of imidophosphate | Bos taurus | |
Mg2+ | or other divalent cation required for hydrolysis of diphosphate | Bos taurus | |
Mn2+ | increases hydrolysis of imidophosphate | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33000, SDS-PAGE | Bos taurus |
56000 | - |
ultracentrifuge method | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Bos taurus | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
diphosphate | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Bos taurus | 2 phosphate | - |
? | |
imidodiphosphate + 2 H2O | hydrolysis even in the absence of divalent cations | Bos taurus | 2 phosphate + NH3 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Bos taurus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | - |
Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
no activity | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
p-chloromercuriphenyl sulfonate | - |
Bos taurus |