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Literature summary for 3.6.1.1 extracted from
- Purification and characterization of thylakoid membrane-bound inorganic pyrophosphatase from Spinacia oleracea L. (1997), Arch. Biochem. Biophys., 346, 105-112.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | less effective than Cd2+ | Spinacia oleracea |  |
| Cd2+ | - |
Spinacia oleracea | |
| Co2+ | less effective than Ca2+ | Spinacia oleracea | |
| Cu2+ | less effective then Mn2+ | Spinacia oleracea | |
| diphosphate | above 1 mM | Spinacia oleracea | |
| F- | - |
Spinacia oleracea | |
| Mn2+ | less effective than Zn2+ | Spinacia oleracea | |
| Zn2+ | less effective then Co2+ | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane bound | Spinacia oleracea | 16020 | - |
| thylakoid | - |
Spinacia oleracea | 9579 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Spinacia oleracea | |
| Mg2+ | no other divalent cations can substitute for Mg2+ | Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
gel filtration, SDS-PAGE, amino acid sequence | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | Spinacia oleracea | actual substrate is magnesium diphosphate or dimagnesium diphosphate | 2 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
- |
Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | actual substrate is magnesium diphosphate or dimagnesium diphosphate | Spinacia oleracea | 2 phosphate | - |
? | |
| diphosphate + H2O | high specificity | Spinacia oleracea | 2 phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 55000, SDS-PAGE | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Spinacia oleracea |
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