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Literature summary for 3.6.1.1 extracted from

  • Josse, J.; Wong, S.C.K.
    Inorganic Pyrophosphatase of Escherichia coli (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 499-527.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2,4,6-Trinitrobenzenesulfonic acid
-
Escherichia coli
Cyanate
-
Escherichia coli
Diazonium-1H-tetrazole
-
Escherichia coli
Guanidine HCl
-
Escherichia coli
NaF
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
Escherichia coli
Mn2+
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
6 * 20000, sedimentation equilibrium Escherichia coli
120000
-
sedimentation equilibrium Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Escherichia coli
-
2 phosphate
-
?
diphosphate + H2O Escherichia coli
-
2 phosphate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
-
Escherichia coli
13
-
crystallized enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
Escherichia coli 2 phosphate
-
?
diphosphate + H2O
-
Escherichia coli 2 phosphate
-
r
diphosphate + H2O reaction may be reversed by coupling to glucose-6-phosphate to 6-phospho-gluconate Escherichia coli 2 phosphate
-
?
diphosphate + H2O reaction may be reversed by coupling to glucose-6-phosphate to 6-phospho-gluconate Escherichia coli 2 phosphate
-
r
tetrapolyphosphate + H2O
-
Escherichia coli ?
-
?
tripolyphosphate + H2O
-
Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 20000, sedimentation equilibrium Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.1
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7 11 20% relative activity at pH 7 and 11 Escherichia coli