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Literature summary for 3.5.99.8 extracted from

  • Qu, Y.; Spain, J.C.
    Molecular and biochemical characterization of the 5-nitroanthranilic acid degradation pathway in Bradyrhizobium sp. strain JS329 (2011), J. Bacteriol., 193, 3057-3063.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene naaA, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain Rosetta 2/pJS803 and in strain EPI300(pJS800) Bradyrhizobium sp.

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl inhibits 70% at 1 mM Bradyrhizobium sp.
EDTA inhibits 99% at 1 mM Bradyrhizobium sp.
additional information the activity of NaaA after inhibition is restored by the addition of divalent metal ions: e.g. Co2+ to 100%, Mn2+ to 85%, Zn2+ to 81%, Fe2+ to 67%, or Ni2+ to 39%, all at 0.667 mM Bradyrhizobium sp.
o-phenanthroline inhibits 98% at 1 mM Bradyrhizobium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ best stimulating divalent cation Bradyrhizobium sp.
additional information NaaA contains the sequence HEAWH (amino acid residues 108 to 112), which is similar to the zinc binding motif HEXXH Bradyrhizobium sp.

Organism

Organism UniProt Comment Textmining
Bradyrhizobium sp. D3WZ85 gene naaA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2/pJS803 by nickel affinity chromatography Bradyrhizobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information mechanism of release of the nitro group, overview. The nitro group is spontaneously eliminated as nitrite concomitant with the formation of a lactone from the ring fission product of 5NSA dioxygenation Bradyrhizobium sp. ?
-
?

Synonyms

Synonyms Comment Organism
5NAA aminohydrolase
-
Bradyrhizobium sp.
5NAA deaminase
-
Bradyrhizobium sp.
NAAA
-
Bradyrhizobium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42 45
-
Bradyrhizobium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Bradyrhizobium sp.

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the initial step in 5-nitroanthranilic acid degradation, a hydrolytic deamination to form 5-nitrosalicylic acid, followed by ring fission catalyzed by 5NSA dioxygenase, biodegradation mechanism and pathway overview Bradyrhizobium sp.
additional information NaaA is a hydrolytic metalloenzyme with a narrow substrate spectrum Bradyrhizobium sp.