Cloned (Comment) | Organism |
---|---|
gene naaA, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain Rosetta 2/pJS803 and in strain EPI300(pJS800) | Bradyrhizobium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | inhibits 70% at 1 mM | Bradyrhizobium sp. | |
EDTA | inhibits 99% at 1 mM | Bradyrhizobium sp. | |
additional information | the activity of NaaA after inhibition is restored by the addition of divalent metal ions: e.g. Co2+ to 100%, Mn2+ to 85%, Zn2+ to 81%, Fe2+ to 67%, or Ni2+ to 39%, all at 0.667 mM | Bradyrhizobium sp. | |
o-phenanthroline | inhibits 98% at 1 mM | Bradyrhizobium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | best stimulating divalent cation | Bradyrhizobium sp. | |
additional information | NaaA contains the sequence HEAWH (amino acid residues 108 to 112), which is similar to the zinc binding motif HEXXH | Bradyrhizobium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bradyrhizobium sp. | D3WZ85 | gene naaA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2/pJS803 by nickel affinity chromatography | Bradyrhizobium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | mechanism of release of the nitro group, overview. The nitro group is spontaneously eliminated as nitrite concomitant with the formation of a lactone from the ring fission product of 5NSA dioxygenation | Bradyrhizobium sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5NAA aminohydrolase | - |
Bradyrhizobium sp. |
5NAA deaminase | - |
Bradyrhizobium sp. |
NAAA | - |
Bradyrhizobium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | 45 | - |
Bradyrhizobium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Bradyrhizobium sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the initial step in 5-nitroanthranilic acid degradation, a hydrolytic deamination to form 5-nitrosalicylic acid, followed by ring fission catalyzed by 5NSA dioxygenase, biodegradation mechanism and pathway overview | Bradyrhizobium sp. |
additional information | NaaA is a hydrolytic metalloenzyme with a narrow substrate spectrum | Bradyrhizobium sp. |