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Literature summary for 3.5.99.7 extracted from

  • Liu, C.H.; Wang, S.A.; Ruszczycky, M.W.; Chen, H.; Li, K.; Murakami, K.; Liu, H.W.
    Studies of 1-amino-2,2-difluorocyclopropane-1-carboxylic acid mechanism of decomposition and inhibition of 1-aminocyclopropane-1-carboxylic acid deaminase (2015), Org. Lett., 17, 3342-3345 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene acdS, recombinant expression of His6-tagged ACCD in Escherichia coli strain BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
1-amino-2,2-difluorocyclopropane-1-carboxylic acid rac-DFACC, chemical synthesis, overview. Mechanism of decomposition and inhibition of 1-aminocyclopropane-1-carboxylic acid deaminase, and Michaelis-Menten kinetics. DFACC is unstable under near-physiological conditions where it primarily decomposes via specific-base catalysis to 3-fluoro-2-oxobut-3-enoic acid. Different decomposotion mechanisms are described. Upon incubation with ACC deaminase, DFACC is a slow-dissociating inhibitor of ACC deaminase with submicromolar affinity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-nitroanthranilate + H2O Escherichia coli
-
5-nitrosalicylate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged ACCD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-nitroanthranilate + H2O
-
Escherichia coli 5-nitrosalicylate + NH3
-
?
additional information 1H NMR spectroscopy for determination of reaction products and metabolites Escherichia coli ?
-
-

Synonyms

Synonyms Comment Organism
ACC deaminase
-
Escherichia coli
ACCD
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics, overview Escherichia coli