Cloned (Comment) | Organism |
---|---|
gene acdS, recombinant expression of His6-tagged ACCD in Escherichia coli strain BL21(DE3) | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-amino-2,2-difluorocyclopropane-1-carboxylic acid | rac-DFACC, chemical synthesis, overview. Mechanism of decomposition and inhibition of 1-aminocyclopropane-1-carboxylic acid deaminase, and Michaelis-Menten kinetics. DFACC is unstable under near-physiological conditions where it primarily decomposes via specific-base catalysis to 3-fluoro-2-oxobut-3-enoic acid. Different decomposotion mechanisms are described. Upon incubation with ACC deaminase, DFACC is a slow-dissociating inhibitor of ACC deaminase with submicromolar affinity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-nitroanthranilate + H2O | Escherichia coli | - |
5-nitrosalicylate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged ACCD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-nitroanthranilate + H2O | - |
Escherichia coli | 5-nitrosalicylate + NH3 | - |
? | |
additional information | 1H NMR spectroscopy for determination of reaction products and metabolites | Escherichia coli | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
ACC deaminase | - |
Escherichia coli |
ACCD | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, overview | Escherichia coli |