Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.99.6 extracted from

  • Hu, G.; Li, L.; Li, D.; Liu, C.; Wei, S.; Liang, Y.; Su, X.
    Protein preparation and preliminary x-ray crystallographic analysis of a putative glucosamine 6-phosphate deaminase from Streptococcus mutans (2007), Acta Crystallogr. Sect. F, F63, 809-811.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.99.6

Cloned(Commentary)

Cloned (Comment) Organism
gene smu.636, cloning from genomic DNA, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) Streptococcus mutans

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme, the protein solution contains 10 mg/ml protein in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5, mixing of 0.001 ml of protein and 0.001 ml of reservoir solution, and equilibration against 0.5 ml of reservoir solution, hanging drop vapour diffusion method, X-ray diffraction structure determination and anaylsis at 2.4 A resolution Streptococcus mutans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25500
-
 x * 25500, recombinant His-tagged enzyme, SDS-PAGE Streptococcus mutans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glucosamine 6-phosphate + H2O Streptococcus mutans
-
 fructose 6-phosphate + NH3
-
 ?
glucosamine 6-phosphate + H2O Streptococcus mutans UA159
-
 fructose 6-phosphate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus mutans
-
 gene smu.636
-
Streptococcus mutans UA159
-
 gene smu.636
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity in two steps by nickel affinity chromatography and gel filtration Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucosamine 6-phosphate + H2O
-
 Streptococcus mutans fructose 6-phosphate + NH3
-
 ?
glucosamine 6-phosphate + H2O
-
 Streptococcus mutans UA159 fructose 6-phosphate + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 25500, recombinant His-tagged enzyme, SDS-PAGE Streptococcus mutans

Synonyms

Synonyms Comment Organism
NagB
-
 Streptococcus mutans
SMU.636 protein
-
 Streptococcus mutans