BRENDA - Enzyme Database
show all sequences of 3.5.99.6

Asymmetric allosteric activation of Escherichia coli glucosamine-6-phosphate deaminase produced by replacement of Tyr121

Altamirano, M.M.; Plumbridge, J.A.; Horjales, H.; Calcagno, M.L.; Biochemistry 34, 6074-6082 (1995)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
C228S
the kinetic and allosteric properties of the mutant enzyme in which Ser replaces Cys219 or Cys228 are the same as those described for the wild-type enzyme. The same result is obtained with the double mutation
Escherichia coli
Y121T
while the wild-type enzyme behaves as a classical allosteric K-system which can be described by the allosteric concerted model, the mutant forms Y121T and Y121W present an asymmetric behaviour towards the allosteric activator, which can be described as two distinct half-of-the-sites allosteric activation steps occuring with different affinities for the N-acetyl-D-glucosamine 6-phosphate
Escherichia coli
Y121W
while the wild-type enzyme behaves as a classical allosteric K-system which can be described by the allosteric concerted model, the mutant forms Y121T and Y121W present an asymmetric behaviour towards the allosteric activator, which can be described as two distinct half-of-the-sites allosteric activation steps occuring with different affinities for the N-acetyl-D-glucosamine 6-phosphate
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.67
-
D-glucosamine 6-phosphate
pH 8.0, 30°C, mutant enzyme Tyr121Trp
Escherichia coli
2.01
-
D-glucosamine 6-phosphate
pH 8.0, 30°C, wild-type enzyme
Escherichia coli
2.6
-
D-glucosamine 6-phosphate
pH 8.0, 30°C, mutant enzyme Tyr121Thr
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucosamine 6-phosphate + H2O
-
648231
Escherichia coli
D-fructose 6-phosphate + NH3
-
648231
Escherichia coli
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C228S
the kinetic and allosteric properties of the mutant enzyme in which Ser replaces Cys219 or Cys228 are the same as those described for the wild-type enzyme. The same result is obtained with the double mutation
Escherichia coli
Y121T
while the wild-type enzyme behaves as a classical allosteric K-system which can be described by the allosteric concerted model, the mutant forms Y121T and Y121W present an asymmetric behaviour towards the allosteric activator, which can be described as two distinct half-of-the-sites allosteric activation steps occuring with different affinities for the N-acetyl-D-glucosamine 6-phosphate
Escherichia coli
Y121W
while the wild-type enzyme behaves as a classical allosteric K-system which can be described by the allosteric concerted model, the mutant forms Y121T and Y121W present an asymmetric behaviour towards the allosteric activator, which can be described as two distinct half-of-the-sites allosteric activation steps occuring with different affinities for the N-acetyl-D-glucosamine 6-phosphate
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.67
-
D-glucosamine 6-phosphate
pH 8.0, 30°C, mutant enzyme Tyr121Trp
Escherichia coli
2.01
-
D-glucosamine 6-phosphate
pH 8.0, 30°C, wild-type enzyme
Escherichia coli
2.6
-
D-glucosamine 6-phosphate
pH 8.0, 30°C, mutant enzyme Tyr121Thr
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucosamine 6-phosphate + H2O
-
648231
Escherichia coli
D-fructose 6-phosphate + NH3
-
648231
Escherichia coli
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Escherichia coli
Other publictions for EC 3.5.99.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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1
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3
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720877
Naderer
Evidence that intracellular st ...
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PLoS Pathog.
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1
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1
1
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710931
Wendland
N-Acetylglucosamine utilizatio ...
Candida albicans, Candida albicans BWP17
Appl. Environ. Microbiol.
75
5840-5845
2009
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1
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5
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712289
Alvarez-Anorve
Allosteric regulation of gluco ...
Escherichia coli
J. Bacteriol.
191
6401-6407
2009
1
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1
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1
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1
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684638
Solar
The role of glucosamine-6-phos ...
Aspergillus niger, Aspergillus niger A158
Appl. Microbiol. Biotechnol.
78
613-619
2008
1
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2
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1
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2
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3
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1
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1
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4
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1
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1
1
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1
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2
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1
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2
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1
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2
1
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4
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1
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1
1
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684212
Hu
Protein preparation and prelim ...
Streptococcus mutans, Streptococcus mutans UA159
Acta Crystallogr. Sect. F
F63
809-811
2007
-
-
1
1
-
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1
2
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20
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1
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2
1
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2
1
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667318
Lucumi-Moreno
On the functional role of Arg1 ...
Escherichia coli
Arch. Biochem. Biophys.
442
41-48
2005
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4
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5
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2
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1
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1
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5
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667624
Bustos-Jaimes
Evidence for two different mec ...
Escherichia coli
Biochemistry
44
1127-1135
2005
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5
-
1
6
-
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-
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2
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1
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1
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6
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5
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1
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6
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1
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6
-
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667638
Sosa-Peinado
Site-directed fluorescence lab ...
Escherichia coli
Biochemistry
44
15083-15092
2005
-
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1
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1
8
-
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2
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1
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7
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6
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1
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1
6
8
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1
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7
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669110
Tanaka
Characterization of a novel gl ...
Thermococcus kodakarensis
J. Bacteriol.
187
7038-7044
2005
-
-
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-
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4
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2
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3
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2
1
1
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2
3
1
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4
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2
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2
1
1
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2
3
1
-
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669338
Vincent
Structure and kinetics of a mo ...
Bacillus subtilis
J. Biol. Chem.
280
19649-19655
2005
-
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1
1
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3
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1
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1
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1
1
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1
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648241
Arreola
Two mammalian glucosamine-6-ph ...
Homo sapiens, Mus musculus
FEBS Lett.
551
63-70
2003
-
-
2
1
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4
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1
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1
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1
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648240
Rudino-Pinera
Structural flexibility, an ess ...
Escherichia coli
Acta Crystallogr. Sect. D
58
10-20
2002
1
-
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1
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1
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2
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1
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1
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2
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648244
Bustos-Jaimes
On the role of the conformatio ...
Escherichia coli
J. Mol. Biol.
319
183-189
2002
-
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1
1
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1
2
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2
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648239
Montero-Moran
On the multiple functional rol ...
Escherichia coli
Biochemistry
40
10187-10196
2001
-
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4
-
6
1
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2
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1
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1
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1
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1
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3
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4
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6
3
1
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1
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1
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1
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648243
Bustos-Jaimes
Allosteric transition and subs ...
Escherichia coli
Arch. Biochem. Biophys.
394
156-160
2001
1
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2
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1
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648238
Lara-Gonzalez
On the role of the N-terminal ...
Escherichia coli
J. Mol. Biol.
301
219-227
2000
1
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1
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1
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1
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648242
Montag
Characterization of testicular ...
Mus musculus
FEBS Lett.
458
141-144
1999
-
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1
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1
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3
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8
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1
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8
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2
1
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648234
Chmara
Antibacterial action of dipept ...
Bacillus subtilis
Microbiology
144
1349-1358
1998
-
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-
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1
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1
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2
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2
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1
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2
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648236
Lara-Lemus
Glucosamine-6-phosphate deamin ...
Bos taurus
Biochim. Biophys. Acta
1388
1-9
1998
1
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1
3
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2
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4
-
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1
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2
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1
1
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3
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1
1
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1
1
3
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1
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2
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1
1
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3
-
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1
-
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-
-
648237
Montero-Moran
Tyr254 hydroxyl group acts as ...
Escherichia coli
Biochemistry
37
7844-7849
1998
1
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2
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2
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1
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1
1
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6
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1
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1
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1
1
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6
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-
648228
Steimle
Purification and characterizat ...
Giardia intestinalis
Mol. Biochem. Parasitol.
84
149-153
1997
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1
1
3
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1
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2
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1
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1
3
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1
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1
1
1
1
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-
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1
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-
648229
Oliva
Structure and catalytic mechan ...
Escherichia coli
Structure
3
1323-1332
1995
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1
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2
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1
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1
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1
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-
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-
648231
Altamirano
Asymmetric allosteric activati ...
Escherichia coli
Biochemistry
34
6074-6082
1995
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-
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3
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3
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2
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1
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1
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3
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3
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1
-
-
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1
-
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-
-
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-
648232
Weidanz
Glucosamine 6-phosphate deamin ...
Homo sapiens
Br. J. Haematol.
91
72-79
1995
3
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9
1
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1
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4
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2
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2
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1
1
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1
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1
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3
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9
-
1
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1
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2
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2
-
1
1
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-
1
-
-
1
-
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-
648230
Altamirano
Glucosamine-6-phosphate deamin ...
Escherichia coli
Biochem. J.
295
645-648
1993
1
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1
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1
1
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-
648235
Horjales
Crystallization and preliminar ...
Escherichia coli
J. Mol. Biol.
226
1283-1286
1992
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1
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3
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1
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671576
Lara-Lemus
Purification and characterizat ...
Canis lupus familiaris
Arch. Biochem. Biophys.
297
213-220
1992
2
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2
2
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2
1
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2
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1
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2
1
1
1
1
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2
1
1
1
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1
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2
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2
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2
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2
1
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1
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2
1
1
1
1
-
-
-
2
1
1
1
1
-
-
-
-
-
-
648233
Altamirano
Zinc binding and its trapping ...
Escherichia coli
Biochim. Biophys. Acta
1038
291-294
1990
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-
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1
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3
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1
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1
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-
-
648227
Altamirano
Sulfhydryl groups of glucosami ...
Escherichia coli
Arch. Biochem. Biophys.
253
95-100
1987
1
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1
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2
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1
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648226
Calcagno
Purification, molecular and ki ...
Escherichia coli
Biochim. Biophys. Acta
787
165-173
1984
1
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4
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3
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3
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1
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1
1
1
1
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2
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1
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4
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3
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1
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1
1
1
1
-
-
-
2
-
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-
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-
-
-
-
648225
Rapsch
-
Glucosamine 6-P isomerase of E ...
Evernia prunastri
Cryptogam Bryol. Lichenol.
4
161-167
1983
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1
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1
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1
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1
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1
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-
648224
Das
-
Purification and some properti ...
Candida albicans
Biochem. Int.
5
735-741
1982
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2
2
1
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1
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1
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1
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1
1
1
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1
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2
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2
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1
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1
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1
1
1
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1
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-
648223
Kikuchi
Stabilization and purification ...
Rattus norvegicus
Sci. Rep. Res. Inst. Tohoku Univ. Med.
26
92-98
1979
-
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1
1
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2
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1
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1
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2
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1
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1
1
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2
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648222
Enghofer
Glucosamine metabolism in Dros ...
Drosophila virilis
Biochim. Biophys. Acta
544
245-261
1978
1
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2
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1
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1
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1
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1
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1
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1
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2
1
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-
648221
Midelfort
Studies on the mechanism of Es ...
Escherichia coli
Biochemistry
16
1590-1596
1977
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1
1
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2
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1
1
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1
1
1
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1
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1
1
1
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1
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1
1
1
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-
-
648219
Friedman
Glucosaminephosphate isomerase ...
Musca domestica, Sarcophaga bullata
Methods Enzymol.
41B
400-407
1975
5
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-
1
4
-
1
1
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2
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1
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1
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2
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2
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5
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1
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4
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1
1
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1
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1
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2
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-
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-
2
-
-
-
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-
-
-
-
-
648220
Withe
N-Acetylglucosamine-6-phosphat ...
Escherichia coli
Methods Enzymol.
41B
497-502
1975
1
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1
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2
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1
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1
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1
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1
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1
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1
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1
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1
-
1
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
648218
Tesoriere
Glucosamine 6-phosphate deamin ...
Oryctolagus cuniculus
Ital. J. Biochem.
19
139-154
1970
-
-
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-
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6
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2
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1
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2
1
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1
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1
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6
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1
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2
1
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1
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-
-
-
-
1
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-
-
-
-
-
-
-
-
648216
Nakada
-
Phosphoglucosamine isomerase f ...
Proteus vulgaris
Methods Enzymol.
9
575-579
1966
1
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-
-
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-
1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
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1
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-
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648217
Pattabiraman
Purification of glucosamine 6- ...
Homo sapiens
Biochim. Biophys. Acta
54
273-283
1961
1
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5
6
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6
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2
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1
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2
1
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1
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1
1
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1
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5
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6
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6
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1
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2
1
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1
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1
1
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648215
Comb
Glucosamine metabolism. IV. Gl ...
Escherichia coli, Sus scrofa
J. Biol. Chem.
232
807-827
1958
3
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4
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2
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2
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1
2
1
2
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4
1
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3
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1
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4
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2
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1
2
1
2
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4
1
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