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Literature summary for 3.5.99.6 extracted from
- Purification and characterization of encystment-induced glucosamine 6-phosphate isomerase in Giardia (1997), Mol. Biochem. Parasitol., 84, 149-153.
General Stability
| General Stability | Organism |
|---|---|
| labile to freezing and thawing | Giardia intestinalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-deoxy-2-amino-D-glucitol 6-phosphate | - |
Giardia intestinalis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.38 | - |
D-glucosamine 6-phosphate | pH 6.82, 39°C | Giardia intestinalis | |
| 2.5 | - |
D-fructose 6-phosphate | pH 8.9, 30°C | Giardia intestinalis | |
| 19 | - |
NH4+ | pH 6.82, 39°C | Giardia intestinalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
gel filtration | Giardia intestinalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Giardia intestinalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Giardia intestinalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 308 | - |
- |
Giardia intestinalis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, stable for up to 2 months | Giardia intestinalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucosamine 6-phosphate + H2O | - |
Giardia intestinalis | D-fructose 6-phosphate + NH3 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 29000, SDS-PAGE | Giardia intestinalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
both directions | Giardia intestinalis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
2-deoxy-2-amino-D-glucitol 6-phosphate | pH 7.8, 25°C, substrate : D-glucosamine-6-phosphate | Giardia intestinalis | |
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Release 2023.1 (January 2023)
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