Any feedback?
Literature summary for 3.5.99.6 extracted from
- Purification, molecular and kinetic properties of glucosamine-6-phosphate isomerase (deaminase) from Escherichia coli (1984), Biochim. Biophys. Acta, 787, 165-173.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| N-acetylglucosamine 6-phosphate | - |
Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
D-fructose 6-phosphate | pH 7.7, 30°C, in presence of 0.5 mM N-acetylglucosamine 6-phosphate | Escherichia coli | |
| 2.2 | - |
D-glucosamine 6-phosphate | in presence of 0.2 mM N-acetylglucosamine 6-phosphate | Escherichia coli | |
| 31.4 | - |
NH4+ | pH 7.7, 30°C, in presence of 0.25 mM N-acetylglucosamine 6-phosphate | Escherichia coli | |
| 35.4 | - |
NH4+ | pH 7.7, 30°C, without activator | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29700 | - |
6 * 29700, SDS-PAGE | Escherichia coli |
| 185000 | - |
gel filtration | Escherichia coli |
| 190000 | - |
pore-gradient gel electrophoresis, density gradient centrifugation | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 606.8 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 150 mM Tris-HCl buffer, pH 7.25, 50% v/v glycerol, stable up to 6 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucosamine 6-phosphate + H2O | - |
Escherichia coli | D-fructose 6-phosphate + NH3 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 29700, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 455 | - |
D-fructose 6-phosphate | pH 7.7, 30°C | Escherichia coli | |
| 1800 | - |
D-glucosamine 6-phosphate | pH 7.7, 30°C | Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
