Activating Compound | Comment | Organism | Structure |
---|---|---|---|
N-Acetylgalactosamine 6-phosphate | activates | Sus scrofa | |
N-acetylglucosamine 6-phosphate | activates | Escherichia coli | |
N-acetylglucosamine 6-phosphate | activates | Sus scrofa | |
N-acetylglucosamine 6-phosphate | increases reaction velocity when D-glucosamine 6-phosphate is present at levels below that required for saturation | Escherichia coli |
General Stability | Organism |
---|---|
labile to freezing and thawing | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.66 | - |
D-glucosamine 6-phosphate | pH 7.9, 37°C, activated by N-acetylglucosamine 6-phosphate | Sus scrofa | |
1.2 | - |
D-glucosamine 6-phosphate | pH 7.8, 37°C, activated by N-acetylglucosamine 6-phosphate | Escherichia coli | |
7.1 | - |
D-glucosamine 6-phosphate | pH 7.8, 37°C | Escherichia coli | |
14 | - |
D-glucosamine 6-phosphate | pH 7.8, 37°C, without activator | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Sus scrofa | - |
hog | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
52 | - |
- |
Sus scrofa |
158 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-18°C, little loss of activity after 2-3 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucosamine 6-phosphate + H2O | - |
Sus scrofa | D-fructose 6-phosphate + NH3 | - |
r | |
D-glucosamine 6-phosphate + H2O | specific for | Escherichia coli | D-fructose 6-phosphate + NH3 | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.2 | - |
Sus scrofa |
7.5 | - |
enzyme activated by N-acetylgalactosamine 6-phosphate | Sus scrofa |
7.8 | - |
- |
Escherichia coli |
8.8 | - |
enzyme activated by N-acetylglucosamine 6-phosphate | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH 6.0: about 25% of maximal activity, pH 8.5: about 60% of maximal activity | Escherichia coli |