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Literature summary for 3.5.99.5 extracted from
- A novel 2-aminomuconate deaminase in the nitrobenzene degradation pathway of Pseudomonas pseudoalcaligenes JS45 (1998), J. Bacteriol., 180, 2502-2506.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | 2 mM, 20% inhibition | Pseudomonas oleovorans |  |
| diethyldicarbonate | 2 mM, complete inhibition | Pseudomonas oleovorans | |
| MnCl2 | 2 mM, 20% inhibition | Pseudomonas oleovorans | |
| additional information | no inhibition by 2-aminomuconic semialdehyde, glycine, alanine, aspartic acid, glutaric acid, 2-aminoadipic acid | Pseudomonas oleovorans | |
| phenylhydrazine | 10 mM, 20% inhibition | Pseudomonas oleovorans | |
| ZnCl2 | 2 mM, 70% inhibition | Pseudomonas oleovorans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.067 | - |
2-aminomuconate | - |
Pseudomonas oleovorans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Pseudomonas oleovorans | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for divalent cations | Pseudomonas oleovorans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16600 | - |
6 * 16600, SDS-PAGE | Pseudomonas oleovorans |
| 100000 | - |
gel filtration | Pseudomonas oleovorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aminomuconate + H2O | Pseudomonas oleovorans | intermediate in biodegradation of nitrobenzene | ? | - |
? | |
| 2-aminomuconate + H2O | Pseudomonas oleovorans JS45 | intermediate in biodegradation of nitrobenzene | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas oleovorans | - |
JS45 | - |
| Pseudomonas oleovorans JS45 | - |
JS45 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas oleovorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37.8 | - |
- |
Pseudomonas oleovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aminomuconate + H2O | - |
Pseudomonas oleovorans | 4-oxalocrotonate + NH3 | - |
ir | |
| 2-aminomuconate + H2O | - |
Pseudomonas oleovorans JS45 | 4-oxalocrotonate + NH3 | - |
ir | |
| 2-aminomuconate + H2O | intermediate in biodegradation of nitrobenzene | Pseudomonas oleovorans | ? | - |
? | |
| 2-aminomuconate + H2O | intermediate in biodegradation of nitrobenzene | Pseudomonas oleovorans JS45 | ? | - |
? | |
| additional information | no activity with 2-aminomuconic semialdehyde, glycine, alanine, aspartic acid, glutaric acid, 2-aminoadipic acid | Pseudomonas oleovorans | ? | - |
? | |
| additional information | no activity with 2-aminomuconic semialdehyde, glycine, alanine, aspartic acid, glutaric acid, 2-aminoadipic acid | Pseudomonas oleovorans JS45 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 16600, SDS-PAGE | Pseudomonas oleovorans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
stable for at least 3 days | Pseudomonas oleovorans |
| 60 | - |
77% loss of activity in 5 min | Pseudomonas oleovorans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 208 | - |
2-aminomuconate | - |
Pseudomonas oleovorans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
- |
Pseudomonas oleovorans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 8.8 | at room temperature for at least 3 h | Pseudomonas oleovorans |
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