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Literature summary for 3.5.99.2 extracted from

  • Begum, A.; Drebes, J.; Perbandt, M.; Wrenger, C.; Betzel, C.
    Purification, crystallization and preliminary X-ray diffraction analysis of the thiaminase type II from Staphylococcus aureus (2011), Acta Crystallogr. Sect. F, 67, 51-53.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.99.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of C-terminally Strep-tagged TenA in Escherichia coli strain BL21(DE3) Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant C-terminally Strep-tagged TenA, hanging drop vapour diffusion method, 0.002 ml of 10 mg/ml protein in 100 mM Tris-HCl buffer pH 8.0, is mixed with 0.002 l of reservoir solution containing 0.2 M sodium acetate, 0.1 M Tris-HCl pH 8.5 and 28% w/v PEG 3350, 20°C, 7 days, X-ray diffraction structure determination and analysis at 2.60 A resolution, molecular replacement, modelling Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26840
-
 x * 26840, sequence calculation Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally Strep-tagged TenA from Escherichia coli strain BL21(DE3) by affinity chromatography Staphylococcus aureus

Subunits

Subunits Comment Organism
? x * 26840, sequence calculation Staphylococcus aureus

Synonyms

Synonyms Comment Organism
TenA
-
 Staphylococcus aureus
thiaminase type II
-
 Staphylococcus aureus

General Information

General Information Comment Organism
metabolism thiaminase type II, TenA, catalyzes the deamination of aminopyrimidines, including the cleavage of thiamine to 4-amino-5-hydroxymethyl-2-methylpyrimidine and 5-(2-hydroxyethyl)-4-methylthiazole in the metabolism of thiamine (vitamin B1), in Staphylococcus aureus Staphylococcus aureus
additional information putative active-site residues are Asp44, Cys137, Tyr167 and Glu208 Staphylococcus aureus