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Literature summary for 3.5.99.2 extracted from

  • Mueller, I.B.; Bergmann, B.; Groves, M.R.; Couto, I.; Amaral, L.; Begley, T.P.; Walter, R.D.; Wrenger, C.
    The vitamin B1 metabolism of Staphylococcus aureus is controlled at enzymatic and transcriptional levels (2009), PLoS ONE, 4, e7656.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BLR (DE3) cells Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.256
-
thiamine apparent value, in 100 mM Tris-HCl, pH 7.5, at 37°C Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82900
-
static light scattering Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0046
-
in 100 mM Tris-HCl, pH 7.5, at 37°C Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information TenA does not accept TMP and TPP as substrates Staphylococcus aureus ?
-
?
thiamine + H2O
-
Staphylococcus aureus 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
?

Subunits

Subunits Comment Organism
trimer in vitro Staphylococcus aureus

Synonyms

Synonyms Comment Organism
TenA
-
Staphylococcus aureus
thiaminase II
-
Staphylococcus aureus
thiaminase type II
-
Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0017
-
thiamine in 100 mM Tris-HCl, pH 7.5, at 37°C Staphylococcus aureus

General Information

General Information Comment Organism
metabolism thiaminase is involved in vitamin B1 metabolism Staphylococcus aureus