Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.99.2 extracted from

  • Barison, N.; Cendron, L.; Trento, A.; Angelini, A.; Zanotti, G.
    Structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway - evidence of a different substrate specificity (2009), FEBS J., 276, 6227-6235.
    View publication on PubMed
Search for more literature for 3.5.99.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method, using 0.1 m Tris (pH 8.5), 1.1 M lithium sulfate Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
F47Y poorly active mutant. At pH 6.0, its activity is absent. Moreover, the enzyme does not present any activity on thiamin degradation Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.058
-
 4-amino-5-aminomethyl-2-methylpyrimidine wild type enzyme, in 30 mM Tris, 50 mM NaCl (pH 8.0), at 20°C Helicobacter pylori
0.068
-
 4-amino-5-aminomethyl-2-methylpyrimidine mutant enzyme F47Y, in 30 mM Tris, 50 mM NaCl (pH 8.0), at 20°C Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 4 * 25000, SDS-PAGE Helicobacter pylori
25643
-
 4 * 25643, calculated from amino acid sequence Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori O25874
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography and Superdex 200 gel filtration Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-amino-5-aminomethyl-2-methylpyrimidine + H2O the wild type enzyme is poorly active on 4-amino-5-aminoethyl-2-methylpyrimidine Helicobacter pylori 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 25000, SDS-PAGE Helicobacter pylori
homotetramer 4 * 25643, calculated from amino acid sequence Helicobacter pylori

Synonyms

Synonyms Comment Organism
HP1287
-
 Helicobacter pylori
TenA
-
 Helicobacter pylori
thiaminase II
-
 Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 4-amino-5-aminomethyl-2-methylpyrimidine mutant enzyme F47Y, in 30 mM Tris, 50 mM NaCl (pH 8.0), at 20°C Helicobacter pylori
0.003
-
 4-amino-5-aminomethyl-2-methylpyrimidine wild type enzyme, in 30 mM Tris, 50 mM NaCl (pH 8.0), at 20°C Helicobacter pylori

General Information

General Information Comment Organism
metabolism TenA plays a pivotal role in the thiamin biosynthetic route Helicobacter pylori