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Literature summary for 3.5.99.11 extracted from

  • Liu, L.; Wu, J.F.; Ma, Y.F.; Wang, S.Y.; Zhao, G.P.; Liu, S.J.
    A novel deaminase involved in chloronitrobenzene and nitrobenzene degradation with Comamonas sp. strain CNB-1 (2007), J. Bacteriol., 189, 2677-2682.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.99.11

Cloned(Commentary)

Cloned (Comment) Organism
-
 Comamonas testosteroni

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 2 mM Comamonas testosteroni
Cu2+ 2 mM, complete inhibition Comamonas testosteroni
diethyl dicarbonate 10 mM Comamonas testosteroni
Mg2+ 2 mM Comamonas testosteroni
Ni2+ 2 mM, complete inhibition Comamonas testosteroni
p-chloromercuribenzoate 0.2 mM, complete inhibition Comamonas testosteroni
Zn2+ 2 mM, complete inhibition Comamonas testosteroni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
 2-aminomuconate pH and temperature not specified in the publication Comamonas testosteroni
0.0088
-
 2-amino-5-chloromuconate pH and temperature not specified in the publication Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 1 * 28000, SDS-PAGE Comamonas testosteroni
30000
-
 gel filtration Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-aminomuconate + H2O Comamonas testosteroni the enzyme is involved in chloronitrobenzene and nitrobenzene degradation (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3
-
 ?
2-aminomuconate + H2O Comamonas testosteroni CNB-1 the enzyme is involved in chloronitrobenzene and nitrobenzene degradation (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-
Comamonas testosteroni CNB-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Comamonas testosteroni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.147
-
 substrate: 2-aminomuconate, pH and temperature not specified in the publication Comamonas testosteroni
0.196
-
 substrate: 2-amino-5-chloromuconate, pH and temperature not specified in the publication Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-5-chloromuconate + H2O
-
 Comamonas testosteroni 2-hydroxy-5-chloromuconate + NH3
-
 ?
2-amino-5-chloromuconate + H2O
-
 Comamonas testosteroni CNB-1 2-hydroxy-5-chloromuconate + NH3
-
 ?
2-aminomuconate + H2O the enzyme is involved in chloronitrobenzene and nitrobenzene degradation Comamonas testosteroni (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3
-
 ?
2-aminomuconate + H2O 2-aminomuconate i.e. (2Z,4E)-2-aminohexa-2,4-dienedioate Comamonas testosteroni (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate i.e. (2Z,4E)-2-hydroxymuconate ?
2-aminomuconate + H2O the enzyme is involved in chloronitrobenzene and nitrobenzene degradation Comamonas testosteroni CNB-1 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3
-
 ?
2-aminomuconate + H2O 2-aminomuconate i.e. (2Z,4E)-2-aminohexa-2,4-dienedioate Comamonas testosteroni CNB-1 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate i.e. (2Z,4E)-2-hydroxymuconate ?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Comamonas testosteroni

Synonyms

Synonyms Comment Organism
cnbZ
-
 Comamonas testosteroni

General Information

General Information Comment Organism
metabolism the enzyme is involved in chloronitrobenzene and nitrobenzene degradation Comamonas testosteroni