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Literature summary for 3.5.99.10 extracted from

  • Shukla, S.; Mahadevan, S.
    The ridA gene of E. coli is indirectly regulated by BglG through the transcriptional regulator Lrp in stationary phase (2019), Microbiology, 165, 683-696 .
    View publication on PubMed
Search for more literature for 3.5.99.10

Cloned(Commentary)

Cloned (Comment) Organism
gene ridA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21, quantitative expression analysis in Bgl- and Bgl+ strain ZK819 derivatives, overview Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AF93 two Bgl- strains ZK819-Tn10 and ZK819-Tn5 are isogenic derivatives of a common Bgl- parent strain ZK819 differing only in their antibiotic resistance markers that have been verified to be neutral. The two Bgl+ strains ZK819-97T and ZK819-bglR::IS1 have also been derived from the same Bgl- parent strain ZK819. The two strains differ only in the nature of the mutation that activates the operon. While ZK819-97T carries a point mutation in the CAP-cAMP binding site of the operon, ZK819-bglR::IS1 carries an IS1 insertion in the bglR regulatory region that activates the operon
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis Escherichia coli

Synonyms

Synonyms Comment Organism
ridA
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 Escherichia coli
yjgF
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 Escherichia coli

Expression

Organism Comment Expression
Escherichia coli leucine antagonizes the activation by inducing transcriptional factor Lrp down
Escherichia coli the ridA gene of Escherichia coli is indirectly regulated by BglG through the transcriptional regulator Lrp in stationary phase. Gene ridA is positively regulated by leucine responsive regulatory protein (Lrp) and leucine antagonizes the activation by Lrp, putative Lrp binding site within the ridA promoter. Purified Lrp protein binds to ridA promoter in vitro up

General Information

General Information Comment Organism
malfunction loss of ridA function in a Bgl+ background results in a significant growth retardation in serine-containing media compared to that in a Bgl- background. Deletion of ridA is more disadvantageous in a Bgl+ background, complex metabolic phenotypes like sensitivity to serine in glucose-rich medium and inability to grow on pyruvate as the sole carbon source, overview Escherichia coli
metabolism the ridA gene of Escherichia coli is indirectly regulated by BglG through the transcriptional regulator Lrp in stationary phase Escherichia coli
physiological function reactive metabolites of enamine/imine nature generated during the breakdown of amino acids like serine and threonine are highly nucleophilic and pose a serious threat to cell viability. RidA deaminates these metabolites and facilitates their conversion into utilizable products, thus preventing cellular damage. Overexpression of ridA in Bgl+ background during stationary phase is physiologically relevant to eliminate toxic metabolites generated by the catabolism of serine-containing peptides as a result of elevated levels of their uptake Escherichia coli