BRENDA - Enzyme Database show
show all sequences of 3.5.5.7

A novel nitrilase from Rhodobacter sphaeroides LHS-305: cloning, heterologous expression and biochemical characterization

Wang, H.; Li, G.; Li, M.; Wei, D.; Wang, X.; World J. Microbiol. Biotechnol. 30, 245-252 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli Rosetta (DE3) cells
Rhodobacter sphaeroides
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
1% residual activity at 5 mM
Rhodobacter sphaeroides
Ca2+
60% residual activity at 5 mM
Rhodobacter sphaeroides
Co2+
75% residual activity at 5 mM
Rhodobacter sphaeroides
Cu2+
43% residual activity at 5 mM
Rhodobacter sphaeroides
EDTA
63% residual activity at 5 mM
Rhodobacter sphaeroides
Fe2+
44% residual activity at 5 mM
Rhodobacter sphaeroides
Mg2+
93% residual activity at 5 mM
Rhodobacter sphaeroides
Ni2+
87% residual activity at 5 mM
Rhodobacter sphaeroides
Zn2+
19% residual activity at 5 mM
Rhodobacter sphaeroides
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
73.1
-
3-Cyanopyridine
at pH 7.0 and 30°C
Rhodobacter sphaeroides
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
14 * 40000, SDS-PAGE
Rhodobacter sphaeroides
560000
-
gel filtration
Rhodobacter sphaeroides
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodobacter sphaeroides
G5DDB2
-
-
Rhodobacter sphaeroides LHS-305
G5DDB2
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography, and gel filtration
Rhodobacter sphaeroides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-dicyanobutane + H2O
114.3% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
?
-
-
-
?
1,8-dicyanooctane + H2O
114.3% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
?
-
-
-
?
2-methylglutaronitrile + H2O
23.3% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
2-methylglutarate + NH3
-
-
-
?
3-cyanopyridine + H2O
100% activity
735340
Rhodobacter sphaeroides
?
-
-
-
?
3-cyanopyridine + H2O
100% activity
735340
Rhodobacter sphaeroides LHS-305
?
-
-
-
?
3-phenylpropionitrile + H2O
147.3% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
3-phenylpropanoate + NH3
-
-
-
?
4-chlorobutyronitrile + H2O
135% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
4-chlorobutyrate + NH3
-
-
-
?
4-chlorobutyronitrile + H2O
135% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides LHS-305
4-chlorobutyrate + NH3
-
-
-
?
acrylonitrile + H2O
25.9% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
acrylate + NH3
-
-
-
?
benzonitrile + H2O
55.4% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
benzoate + NH3
-
-
-
?
benzonitrile + H2O
55.4% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides LHS-305
benzoate + NH3
-
-
-
?
dodecanenitrile + H2O
13.5% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
dodecanoate + NH3
-
-
-
?
fumaronitrile + H2O
209.8 activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
fumarate + NH3
-
-
-
?
additional information
no activity with iminodiacetonitrile, glycolonitrile, 3-hydroxyglutaronitrile, phenylacetonitrile, 1,2-phenylenediacetonitrile, mandelonitrile, and indole-3-acetonitrile
735340
Rhodobacter sphaeroides
?
-
-
-
-
additional information
no activity with iminodiacetonitrile, glycolonitrile, 3-hydroxyglutaronitrile, phenylacetonitrile, 1,2-phenylenediacetonitrile, mandelonitrile, and indole-3-acetonitrile
735340
Rhodobacter sphaeroides LHS-305
?
-
-
-
-
pentanenitrile + H2O
62% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
pentanoate + NH3
-
-
-
?
succinonitrile + H2O
66.3% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
succinate + NH3
-
-
-
?
succinonitrile + H2O
66.3% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides LHS-305
succinate + NH3
-
-
-
?
thiophene-3-carbonitrile + H2O
51.2% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetradecamer
14 * 40000, SDS-PAGE
Rhodobacter sphaeroides
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Rhodobacter sphaeroides
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
50
about 55% activity at 30°C, about 80% activity at 35°C, 100% activity at 40°C, about 70% activity at 45°C, and about 30% activity at 50°C
Rhodobacter sphaeroides
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Rhodobacter sphaeroides
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8
about 60% activity at pH 6.0 and 80% activity at pH 8.0 in 10 mM sodium phosphate
Rhodobacter sphaeroides
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Rosetta (DE3) cells
Rhodobacter sphaeroides
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
1% residual activity at 5 mM
Rhodobacter sphaeroides
Ca2+
60% residual activity at 5 mM
Rhodobacter sphaeroides
Co2+
75% residual activity at 5 mM
Rhodobacter sphaeroides
Cu2+
43% residual activity at 5 mM
Rhodobacter sphaeroides
EDTA
63% residual activity at 5 mM
Rhodobacter sphaeroides
Fe2+
44% residual activity at 5 mM
Rhodobacter sphaeroides
Mg2+
93% residual activity at 5 mM
Rhodobacter sphaeroides
Ni2+
87% residual activity at 5 mM
Rhodobacter sphaeroides
Zn2+
19% residual activity at 5 mM
Rhodobacter sphaeroides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
73.1
-
3-Cyanopyridine
at pH 7.0 and 30°C
Rhodobacter sphaeroides
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
14 * 40000, SDS-PAGE
Rhodobacter sphaeroides
560000
-
gel filtration
Rhodobacter sphaeroides
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography, and gel filtration
Rhodobacter sphaeroides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-dicyanobutane + H2O
114.3% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
?
-
-
-
?
1,8-dicyanooctane + H2O
114.3% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
?
-
-
-
?
2-methylglutaronitrile + H2O
23.3% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
2-methylglutarate + NH3
-
-
-
?
3-cyanopyridine + H2O
100% activity
735340
Rhodobacter sphaeroides
?
-
-
-
?
3-cyanopyridine + H2O
100% activity
735340
Rhodobacter sphaeroides LHS-305
?
-
-
-
?
3-phenylpropionitrile + H2O
147.3% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
3-phenylpropanoate + NH3
-
-
-
?
4-chlorobutyronitrile + H2O
135% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
4-chlorobutyrate + NH3
-
-
-
?
4-chlorobutyronitrile + H2O
135% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides LHS-305
4-chlorobutyrate + NH3
-
-
-
?
acrylonitrile + H2O
25.9% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
acrylate + NH3
-
-
-
?
benzonitrile + H2O
55.4% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
benzoate + NH3
-
-
-
?
benzonitrile + H2O
55.4% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides LHS-305
benzoate + NH3
-
-
-
?
dodecanenitrile + H2O
13.5% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
dodecanoate + NH3
-
-
-
?
fumaronitrile + H2O
209.8 activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
fumarate + NH3
-
-
-
?
additional information
no activity with iminodiacetonitrile, glycolonitrile, 3-hydroxyglutaronitrile, phenylacetonitrile, 1,2-phenylenediacetonitrile, mandelonitrile, and indole-3-acetonitrile
735340
Rhodobacter sphaeroides
?
-
-
-
-
additional information
no activity with iminodiacetonitrile, glycolonitrile, 3-hydroxyglutaronitrile, phenylacetonitrile, 1,2-phenylenediacetonitrile, mandelonitrile, and indole-3-acetonitrile
735340
Rhodobacter sphaeroides LHS-305
?
-
-
-
-
pentanenitrile + H2O
62% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
pentanoate + NH3
-
-
-
?
succinonitrile + H2O
66.3% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
succinate + NH3
-
-
-
?
succinonitrile + H2O
66.3% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides LHS-305
succinate + NH3
-
-
-
?
thiophene-3-carbonitrile + H2O
51.2% activity compared to 3-cyanopyridine
735340
Rhodobacter sphaeroides
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetradecamer
14 * 40000, SDS-PAGE
Rhodobacter sphaeroides
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Rhodobacter sphaeroides
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
50
about 55% activity at 30°C, about 80% activity at 35°C, 100% activity at 40°C, about 70% activity at 45°C, and about 30% activity at 50°C
Rhodobacter sphaeroides
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Rhodobacter sphaeroides
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8
about 60% activity at pH 6.0 and 80% activity at pH 8.0 in 10 mM sodium phosphate
Rhodobacter sphaeroides
Other publictions for EC 3.5.5.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733526
Fang
Enzymatic degradation of aliph ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous BX2
Biores. Technol.
185
28-34
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733919
Yusuf
Cloning and functional charact ...
Fusarium proliferatum, Fusarium proliferatum AUF-2
Funct. Integr. Genomics
15
413-424
2015
1
-
1
-
-
-
12
1
-
6
1
-
-
2
-
-
1
-
-
-
-
-
22
1
1
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
12
-
1
-
6
1
-
-
-
-
1
-
-
-
-
22
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
734577
Zhang
Structural insights into enzym ...
Synechocystis sp.
J. Struct. Biol.
188
93-101
2014
-
-
1
1
25
-
-
18
-
-
-
-
-
1
-
-
1
-
-
-
18
-
5
-
-
-
-
18
-
-
-
-
-
-
-
-
-
1
-
1
25
-
-
-
-
18
-
-
-
-
-
-
-
1
-
-
18
-
5
-
-
-
-
18
-
-
-
-
-
-
-
-
18
18
735340
Wang
A novel nitrilase from Rhodoba ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides LHS-305
World J. Microbiol. Biotechnol.
30
245-252
2014
-
-
1
-
-
-
9
1
-
-
2
-
-
2
-
-
1
-
-
-
-
-
19
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
9
-
1
-
-
2
-
-
-
-
1
-
-
-
-
19
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
710984
Bayer
A nitrilase from a metagenomic ...
uncultured bacterium
Appl. Microbiol. Biotechnol.
89
91-98
2011
-
-
1
-
-
-
4
1
-
-
2
-
-
2
-
-
1
-
-
-
-
-
13
1
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
1
-
-
2
-
-
-
-
1
-
-
-
-
13
1
1
-
1
-
1
1
-
-
-
-
-
-
1
1
718661
He
A high-throughput screening st ...
Alcaligenes sp., Alcaligenes sp. ECU0401, Rhodococcus erythropolis, Rhodococcus erythropolis CGMCC 1.2362
Appl. Microbiol. Biotechnol.
89
817-823
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
2
-
12
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
12
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
695831
Kim
Identification and characteriz ...
Pseudomonas fluorescens, Pseudomonas fluorescens Pf-5
Appl. Microbiol. Biotechnol.
83
273-283
2009
-
-
1
-
-
-
6
7
-
-
2
-
-
5
-
-
-
-
-
-
2
-
13
1
1
-
5
7
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
6
-
7
-
-
2
-
-
-
-
-
-
-
2
-
13
1
1
-
5
7
1
-
-
1
-
-
-
-
-
-
699065
Nigam
Nitrilase-catalysed conversion ...
Streptomyces sp.
J. Biosci.
34
21-26
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712260
Thuku
Microbial nitrilases: Versatil ...
Acidovorax facilis, Acidovorax facilis 72W, Acinetobacter sp., Acinetobacter sp. AK226, Comamonas testosteroni, Pseudomonas sp., Pseudomonas sp. S1, Pyrococcus abyssi, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus rhodochrous K22, Synechocystis sp.
J. Appl. Microbiol.
106
703-727
2009
1
2
-
-
3
-
-
-
-
-
14
-
-
22
-
-
-
-
-
-
-
-
29
8
7
-
-
-
7
-
-
-
-
-
-
1
2
-
-
-
3
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
29
8
7
-
-
-
7
-
-
-
-
-
-
-
-
-
712870
Sharma
-
In silico analysis of amino ac ...
Bradyrhizobium sp., Bradyrhizobium sp. ORS278, Methylibium petroleiphilum, Pseudomonas syringae pv. syringae, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus rhodochrous K22, Synechococcus elongatus
J. Proteomics Bioinform.
2
185-192
2009
-
-
-
-
-
-
-
-
-
-
6
-
-
27
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
685014
Yeom
A determinant residue of subst ...
Rhodococcus rhodochrous
Biochem. J.
415
401-407
2008
-
-
1
1
16
-
-
31
-
-
1
-
1
2
-
-
1
1
-
-
13
-
9
1
-
-
-
32
-
-
-
-
-
-
-
-
-
1
-
1
25
-
-
-
-
32
-
-
1
-
1
-
-
1
-
-
13
-
9
1
-
-
-
32
-
-
-
-
-
-
-
-
-
-
695708
Luo
Gene cloning, overexpression, ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous tg1-A6
Appl. Biochem. Biotechnol.
160
393-400
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
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-
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10
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1
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1
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1
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10
-
1
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-
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1
-
-
-
-
-
-
-
-
-
687941
Khandelwal
-
Optimization of nitrilase prod ...
Streptomyces sp., Streptomyces sp. MTCC 7546
J. Chem. Technol. Biotechnol.
82
646-651
2007
2
-
-
-
-
-
-
-
-
-
-
-
-
2
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20
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1
1
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1
1
1
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2
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-
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-
-
-
-
-
20
-
1
1
-
-
1
1
1
-
-
-
-
-
-
-
670463
Bergeron
-
Nitrilase-catalyzed desymmetri ...
Pseudomonas fluorescens
Org. Proc. Res. Dev.
10
661-665
2006
-
1
-
-
-
-
-
-
-
-
-
-
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670792
Mueller
Cloning, overexpression, and c ...
no activity in Aeropyrum pernix, no activity in Pyrococcus furiosus, no activity in Pyrococcus horikoshii, Pyrococcus abyssi, Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543
Protein Expr. Purif.
47
672-681
2006
4
-
1
-
-
-
24
2
1
-
4
-
-
6
-
-
1
-
-
-
3
-
10
2
1
1
6
-
1
1
-
-
-
1
-
4
-
1
-
-
-
-
-
24
-
2
1
-
4
-
-
-
-
1
-
-
3
-
10
2
1
1
6
-
1
1
-
1
-
-
-
-
-
-
685425
Holtze
Transformation of the herbicid ...
Pseudomonas fluorescens 11387, Pseudomonas fluorescens, Pseudomonas putida 11388, Pseudomonas putida, Rhizobium sp. 11401, Rhizobium sp.
Biodegradation
17
503-510
2006
-
-
-
-
-
-
-
-
-
-
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6
-
-
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-
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6
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6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686545
Mukherjee
-
Exploring the synthetic applic ...
Synechocystis sp.
Eur. J. Org. Chem.
23
5238-5242
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
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8
-
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-
-
-
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-
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1
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1
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-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670922
Hann
-
Regioselective biocatalytic hy ...
Acidovorax facilis, Acidovorax facilis 72W
Tetrahedron
60
577-581
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
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2
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-
-
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-
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-
-
-
-
-
-
2
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
654298
Chauhan
Purification, cloning, sequenc ...
Acidovorax facilis, Acidovorax facilis 72W
Appl. Microbiol. Biotechnol.
61
118-122
2003
-
1
1
-
-
-
-
-
-
-
-
-
-
10
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655310
Brenner
Catalysis in the nitrilase sup ...
Arabidopsis thaliana, Rhodococcus rhodochrous
Curr. Opin. Struct. Biol.
12
775-782
2002
-
-
-
-
-
-
-
-
-
-
-
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2
-
-
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2
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3
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-
-
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3
-
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-
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-
-
-
-
-
-
655149
Dias
Enzymatic degradation of nitri ...
Meyerozyma guilliermondii, Meyerozyma guilliermondii UFMG-Y65
Can. J. Microbiol.
46
525-531
2000
-
2
1
-
-
-
1
-
1
-
-
-
-
2
-
-
-
-
-
1
20
-
26
-
1
-
1
-
1
-
-
-
-
-
1
-
2
1
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
1
20
-
26
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
209747
Dhillon
-
Transformation of aliphatic an ...
Pseudomonas sp., Pseudomonas sp. S1
Can. J. Microbiol.
45
811-815
1999
-
-
-
-
-
-
-
6
-
-
1
-
-
3
-
-
1
1
-
-
1
-
15
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
1
-
-
1
-
15
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
32507
Levy-Schil
Aliphatic nitrilase from a soi ...
Comamonas testosteroni
Gene
161
15-20
1995
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
32505
Kobayashi
Primary structure of an alipha ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous K22
Biochemistry
31
9000-9007
1992
-
-
1
-
2
-
-
-
-
-
1
-
-
10
-
-
1
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
32506
Kobayashi
Purification and characterizat ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous K22
J. Bacteriol.
172
4807-4815
1990
-
-
-
-
-
-
3
2
-
1
3
-
-
6
-
-
1
-
-
-
1
-
63
2
1
1
7
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
1
3
-
-
-
-
1
-
-
1
-
63
2
1
1
7
-
1
-
2
-
-
-
-
-
-
-