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Literature summary for 3.5.5.7 extracted from

  • Yusuf, F.; Rather, I.A.; Jamwal, U.; Gandhi, S.G.; Chaubey, A.
    Cloning and functional characterization of nitrilase from Fusarium proliferatum AUF-2 for detoxification of nitriles (2015), Funct. Integr. Genomics, 15, 413-424.
    View publication on PubMed
Search for more literature for 3.5.5.7

Activating Compound

Activating Compound Comment Organism Structure
EDTA about 130% activity at 1 mM Fusarium proliferatum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Fusarium proliferatum

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 1 mM Fusarium proliferatum
Ba2+ about 55% residual activity at 1 mM Fusarium proliferatum
Co2+ about 65% residual activity at 1 mM Fusarium proliferatum
Cu2+ about 75% residual activity at 1 mM Fusarium proliferatum
Dichloromethane about 30% residual activity at 5% (v/v) Fusarium proliferatum
ethanol about 55% residual activity at 5% (v/v) Fusarium proliferatum
hexane about 20% residual activity at 5% (v/v) Fusarium proliferatum
Li+ about 80% residual activity at 1 mM Fusarium proliferatum
methanol about 75% residual activity at 5% (v/v) Fusarium proliferatum
Ni2+ about 60% residual activity at 1 mM Fusarium proliferatum
Propanol about 22% residual activity at 5% (v/v) Fusarium proliferatum
Toluene about 5% residual activity at 5% (v/v) Fusarium proliferatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.55
-
 Benzonitrile at pH 8.0 and 37°C Fusarium proliferatum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ about 115% activity at 1 mM Fusarium proliferatum
Fe2+ about 110% activity at 1 mM Fusarium proliferatum
Fe3+ about 125% activity at 1 mM Fusarium proliferatum
Mg2+ about 125% activity at 1 mM Fusarium proliferatum
Mn2+ about 120% activity at 1 mM Fusarium proliferatum
Zn2+ about 120% activity at 1 mM Fusarium proliferatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, SDS-PAGE Fusarium proliferatum

Organism

Organism UniProt Comment Textmining
Fusarium proliferatum A0A0E3D8K7
-
-
Fusarium proliferatum AUF-2 A0A0E3D8K7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Fusarium proliferatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-cyanopyridine + 2 H2O 110% activity compared to benzonitrile Fusarium proliferatum pyridine 2-carboxylic acid + NH3
-
 ?
3-aminobutyronitrile + H2O 201% activity compared to benzonitrile Fusarium proliferatum 3-aminobutyric acid + NH3
-
 ?
3-cyanopyridine + 2 H2O 151% activity compared to benzonitrile Fusarium proliferatum pyridine 3-carboxylic acid + NH3
-
 ?
3-nitrobenzonitrile + H2O 110% activity compared to benzonitrile Fusarium proliferatum 3-nitrobenzoic acid + NH3
-
 ?
3-phenylpropionitrile + H2O 151% activity compared to benzonitrile Fusarium proliferatum 3-phenylpropionic acid + NH3
-
 ?
3-phenylpropionitrile + H2O 151% activity compared to benzonitrile Fusarium proliferatum AUF-2 3-phenylpropionic acid + NH3
-
 ?
4-aminobutyronitrile + H2O 251% activity compared to benzonitrile Fusarium proliferatum 4-aminobutyric acid + NH3
-
 ?
4-cyanopyridine + 2 H2O 225% activity compared to benzonitrile Fusarium proliferatum pyridine 4-carboxylic acid + NH3
-
 ?
4-nitrobenzonitrile + H2O 151% activity compared to benzonitrile Fusarium proliferatum 4-nitrobenzoic acid + NH3
-
 ?
acetonitrile + H2O 135% activity compared to benzonitrile Fusarium proliferatum acetate + NH3
-
 ?
acetonitrile + H2O 135% activity compared to benzonitrile Fusarium proliferatum AUF-2 acetate + NH3
-
 ?
acrylonitrile + H2O 400% activity compared to benzonitrile Fusarium proliferatum acrylic acid + NH3
-
 ?
acrylonitrile + H2O 400% activity compared to benzonitrile Fusarium proliferatum AUF-2 acrylic acid + NH3
-
 ?
benzonitrile + 2 H2O 100% activity Fusarium proliferatum benzoate + NH3
-
 ?
butyronitrile + H2O 425% activity compared to benzonitrile Fusarium proliferatum butyrate + NH3
-
 ?
diphenylacetonitrile + H2O 72% activity compared to benzonitrile Fusarium proliferatum diphenylacetic acid + NH3
-
 ?
glutaronitrile + H2O 319% activity compared to benzonitrile Fusarium proliferatum glutaric acid + NH3
-
 ?
mandelonitrile + 2 H2O 69% activity compared to benzonitrile Fusarium proliferatum mandelic acid + NH3
-
 ?
mandelonitrile + 2 H2O 69% activity compared to benzonitrile Fusarium proliferatum AUF-2 mandelic acid + NH3
-
 ?
additional information no activity with fumaronitrile and succinonitrile Fusarium proliferatum ?
-
 ?
additional information no activity with fumaronitrile and succinonitrile Fusarium proliferatum AUF-2 ?
-
 ?
propionitrile + H2O 416% activity Fusarium proliferatum propionic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Fusarium proliferatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Fusarium proliferatum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 more than 60% activity between 25 and 45°C Fusarium proliferatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Fusarium proliferatum