BRENDA - Enzyme Database show
show all sequences of 3.5.5.7

Cloning and functional characterization of nitrilase from Fusarium proliferatum AUF-2 for detoxification of nitriles

Yusuf, F.; Rather, I.A.; Jamwal, U.; Gandhi, S.G.; Chaubey, A.; Funct. Integr. Genomics 15, 413-424 (2015)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
about 130% activity at 1 mM
Fusarium proliferatum
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21 (DE3) cells
Fusarium proliferatum
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
complete inhibition at 1 mM
Fusarium proliferatum
Ba2+
about 55% residual activity at 1 mM
Fusarium proliferatum
Co2+
about 65% residual activity at 1 mM
Fusarium proliferatum
Cu2+
about 75% residual activity at 1 mM
Fusarium proliferatum
Dichloromethane
about 30% residual activity at 5% (v/v)
Fusarium proliferatum
ethanol
about 55% residual activity at 5% (v/v)
Fusarium proliferatum
hexane
about 20% residual activity at 5% (v/v)
Fusarium proliferatum
Li+
about 80% residual activity at 1 mM
Fusarium proliferatum
methanol
about 75% residual activity at 5% (v/v)
Fusarium proliferatum
Ni2+
about 60% residual activity at 1 mM
Fusarium proliferatum
Propanol
about 22% residual activity at 5% (v/v)
Fusarium proliferatum
Toluene
about 5% residual activity at 5% (v/v)
Fusarium proliferatum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.55
-
Benzonitrile
at pH 8.0 and 37°C
Fusarium proliferatum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
about 115% activity at 1 mM
Fusarium proliferatum
Fe2+
about 110% activity at 1 mM
Fusarium proliferatum
Fe3+
about 125% activity at 1 mM
Fusarium proliferatum
Mg2+
about 125% activity at 1 mM
Fusarium proliferatum
Mn2+
about 120% activity at 1 mM
Fusarium proliferatum
Zn2+
about 120% activity at 1 mM
Fusarium proliferatum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
x * 37000, SDS-PAGE
Fusarium proliferatum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fusarium proliferatum
A0A0E3D8K7
-
-
Fusarium proliferatum AUF-2
A0A0E3D8K7
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography
Fusarium proliferatum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-cyanopyridine + H2O
110% activity compared to benzonitrile
733919
Fusarium proliferatum
?
-
-
-
?
3-aminobutyronitrile + H2O
201% activity compared to benzonitrile
733919
Fusarium proliferatum
3-aminobutyric acid + NH3
-
-
-
?
3-cyanopyridine + H2O
151% activity compared to benzonitrile
733919
Fusarium proliferatum
?
-
-
-
?
3-nitrobenzonitrile + H2O
110% activity compared to benzonitrile
733919
Fusarium proliferatum
3-nitrobenzoic acid + NH3
-
-
-
?
3-phenylpropionitrile + H2O
151% activity compared to benzonitrile
733919
Fusarium proliferatum
3-phenylpropionic acid + NH3
-
-
-
?
3-phenylpropionitrile + H2O
151% activity compared to benzonitrile
733919
Fusarium proliferatum AUF-2
3-phenylpropionic acid + NH3
-
-
-
?
4-aminobutyronitrile + H2O
251% activity compared to benzonitrile
733919
Fusarium proliferatum
4-aminobutyric acid + NH3
-
-
-
?
4-cyanopyridine + H2O
225% activity compared to benzonitrile
733919
Fusarium proliferatum
?
-
-
-
?
4-nitrobenzonitrile + H2O
151% activity compared to benzonitrile
733919
Fusarium proliferatum
4-nitrobenzoic acid + NH3
-
-
-
?
acetonitrile + H2O
135% activity compared to benzonitrile
733919
Fusarium proliferatum
acetate + NH3
-
-
-
?
acetonitrile + H2O
135% activity compared to benzonitrile
733919
Fusarium proliferatum AUF-2
acetate + NH3
-
-
-
?
acrylonitrile + H2O
400% activity compared to benzonitrile
733919
Fusarium proliferatum
acrylic acid + NH3
-
-
-
?
acrylonitrile + H2O
400% activity compared to benzonitrile
733919
Fusarium proliferatum AUF-2
acrylic acid + NH3
-
-
-
?
benzonitrile + H2O
100% activity
733919
Fusarium proliferatum
benzoate + NH3
-
-
-
?
butyronitrile + H2O
425% activity compared to benzonitrile
733919
Fusarium proliferatum
butyrate + NH3
-
-
-
?
butyronitrile + H2O
425% activity compared to benzonitrile
733919
Fusarium proliferatum AUF-2
butyrate + NH3
-
-
-
?
diphenylacetonitrile + H2O
72% activity compared to benzonitrile
733919
Fusarium proliferatum
diphenylacetic acid + NH3
-
-
-
?
glutaronitrile + H2O
319% activity compared to benzonitrile
733919
Fusarium proliferatum
glutaric acid + NH3
-
-
-
?
mandelonitrile + H2O
69% activity compared to benzonitrile
733919
Fusarium proliferatum
mandelic acid + NH3
-
-
-
?
additional information
no activity with fumaronitrile and succinonitrile
733919
Fusarium proliferatum
?
-
-
-
-
additional information
no activity with fumaronitrile and succinonitrile
733919
Fusarium proliferatum AUF-2
?
-
-
-
-
propionitrile + H2O
416% activity
733919
Fusarium proliferatum
propionic acid + NH3
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 37000, SDS-PAGE
Fusarium proliferatum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Fusarium proliferatum
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
45
more than 60% activity between 25 and 45°C
Fusarium proliferatum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Fusarium proliferatum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
about 130% activity at 1 mM
Fusarium proliferatum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 (DE3) cells
Fusarium proliferatum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
complete inhibition at 1 mM
Fusarium proliferatum
Ba2+
about 55% residual activity at 1 mM
Fusarium proliferatum
Co2+
about 65% residual activity at 1 mM
Fusarium proliferatum
Cu2+
about 75% residual activity at 1 mM
Fusarium proliferatum
Dichloromethane
about 30% residual activity at 5% (v/v)
Fusarium proliferatum
ethanol
about 55% residual activity at 5% (v/v)
Fusarium proliferatum
hexane
about 20% residual activity at 5% (v/v)
Fusarium proliferatum
Li+
about 80% residual activity at 1 mM
Fusarium proliferatum
methanol
about 75% residual activity at 5% (v/v)
Fusarium proliferatum
Ni2+
about 60% residual activity at 1 mM
Fusarium proliferatum
Propanol
about 22% residual activity at 5% (v/v)
Fusarium proliferatum
Toluene
about 5% residual activity at 5% (v/v)
Fusarium proliferatum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.55
-
Benzonitrile
at pH 8.0 and 37°C
Fusarium proliferatum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
about 115% activity at 1 mM
Fusarium proliferatum
Fe2+
about 110% activity at 1 mM
Fusarium proliferatum
Fe3+
about 125% activity at 1 mM
Fusarium proliferatum
Mg2+
about 125% activity at 1 mM
Fusarium proliferatum
Mn2+
about 120% activity at 1 mM
Fusarium proliferatum
Zn2+
about 120% activity at 1 mM
Fusarium proliferatum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
x * 37000, SDS-PAGE
Fusarium proliferatum
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Fusarium proliferatum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-cyanopyridine + H2O
110% activity compared to benzonitrile
733919
Fusarium proliferatum
?
-
-
-
?
3-aminobutyronitrile + H2O
201% activity compared to benzonitrile
733919
Fusarium proliferatum
3-aminobutyric acid + NH3
-
-
-
?
3-cyanopyridine + H2O
151% activity compared to benzonitrile
733919
Fusarium proliferatum
?
-
-
-
?
3-nitrobenzonitrile + H2O
110% activity compared to benzonitrile
733919
Fusarium proliferatum
3-nitrobenzoic acid + NH3
-
-
-
?
3-phenylpropionitrile + H2O
151% activity compared to benzonitrile
733919
Fusarium proliferatum
3-phenylpropionic acid + NH3
-
-
-
?
3-phenylpropionitrile + H2O
151% activity compared to benzonitrile
733919
Fusarium proliferatum AUF-2
3-phenylpropionic acid + NH3
-
-
-
?
4-aminobutyronitrile + H2O
251% activity compared to benzonitrile
733919
Fusarium proliferatum
4-aminobutyric acid + NH3
-
-
-
?
4-cyanopyridine + H2O
225% activity compared to benzonitrile
733919
Fusarium proliferatum
?
-
-
-
?
4-nitrobenzonitrile + H2O
151% activity compared to benzonitrile
733919
Fusarium proliferatum
4-nitrobenzoic acid + NH3
-
-
-
?
acetonitrile + H2O
135% activity compared to benzonitrile
733919
Fusarium proliferatum
acetate + NH3
-
-
-
?
acetonitrile + H2O
135% activity compared to benzonitrile
733919
Fusarium proliferatum AUF-2
acetate + NH3
-
-
-
?
acrylonitrile + H2O
400% activity compared to benzonitrile
733919
Fusarium proliferatum
acrylic acid + NH3
-
-
-
?
acrylonitrile + H2O
400% activity compared to benzonitrile
733919
Fusarium proliferatum AUF-2
acrylic acid + NH3
-
-
-
?
benzonitrile + H2O
100% activity
733919
Fusarium proliferatum
benzoate + NH3
-
-
-
?
butyronitrile + H2O
425% activity compared to benzonitrile
733919
Fusarium proliferatum
butyrate + NH3
-
-
-
?
butyronitrile + H2O
425% activity compared to benzonitrile
733919
Fusarium proliferatum AUF-2
butyrate + NH3
-
-
-
?
diphenylacetonitrile + H2O
72% activity compared to benzonitrile
733919
Fusarium proliferatum
diphenylacetic acid + NH3
-
-
-
?
glutaronitrile + H2O
319% activity compared to benzonitrile
733919
Fusarium proliferatum
glutaric acid + NH3
-
-
-
?
mandelonitrile + H2O
69% activity compared to benzonitrile
733919
Fusarium proliferatum
mandelic acid + NH3
-
-
-
?
additional information
no activity with fumaronitrile and succinonitrile
733919
Fusarium proliferatum
?
-
-
-
-
additional information
no activity with fumaronitrile and succinonitrile
733919
Fusarium proliferatum AUF-2
?
-
-
-
-
propionitrile + H2O
416% activity
733919
Fusarium proliferatum
propionic acid + NH3
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 37000, SDS-PAGE
Fusarium proliferatum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Fusarium proliferatum
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
45
more than 60% activity between 25 and 45°C
Fusarium proliferatum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Fusarium proliferatum
Other publictions for EC 3.5.5.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733526
Fang
Enzymatic degradation of aliph ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous BX2
Biores. Technol.
185
28-34
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733919
Yusuf
Cloning and functional charact ...
Fusarium proliferatum, Fusarium proliferatum AUF-2
Funct. Integr. Genomics
15
413-424
2015
1
-
1
-
-
-
12
1
-
6
1
-
-
2
-
-
1
-
-
-
-
-
22
1
1
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
12
-
1
-
6
1
-
-
-
-
1
-
-
-
-
22
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
734577
Zhang
Structural insights into enzym ...
Synechocystis sp.
J. Struct. Biol.
188
93-101
2014
-
-
1
1
25
-
-
18
-
-
-
-
-
1
-
-
1
-
-
-
18
-
5
-
-
-
-
18
-
-
-
-
-
-
-
-
-
1
-
1
25
-
-
-
-
18
-
-
-
-
-
-
-
1
-
-
18
-
5
-
-
-
-
18
-
-
-
-
-
-
-
-
18
18
735340
Wang
A novel nitrilase from Rhodoba ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides LHS-305
World J. Microbiol. Biotechnol.
30
245-252
2014
-
-
1
-
-
-
9
1
-
-
2
-
-
2
-
-
1
-
-
-
-
-
19
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
9
-
1
-
-
2
-
-
-
-
1
-
-
-
-
19
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
710984
Bayer
A nitrilase from a metagenomic ...
uncultured bacterium
Appl. Microbiol. Biotechnol.
89
91-98
2011
-
-
1
-
-
-
4
1
-
-
2
-
-
2
-
-
1
-
-
-
-
-
13
1
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
1
-
-
2
-
-
-
-
1
-
-
-
-
13
1
1
-
1
-
1
1
-
-
-
-
-
-
1
1
718661
He
A high-throughput screening st ...
Alcaligenes sp., Alcaligenes sp. ECU0401, Rhodococcus erythropolis, Rhodococcus erythropolis CGMCC 1.2362
Appl. Microbiol. Biotechnol.
89
817-823
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
2
-
12
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
12
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
695831
Kim
Identification and characteriz ...
Pseudomonas fluorescens, Pseudomonas fluorescens Pf-5
Appl. Microbiol. Biotechnol.
83
273-283
2009
-
-
1
-
-
-
6
7
-
-
2
-
-
5
-
-
-
-
-
-
2
-
13
1
1
-
5
7
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
6
-
7
-
-
2
-
-
-
-
-
-
-
2
-
13
1
1
-
5
7
1
-
-
1
-
-
-
-
-
-
699065
Nigam
Nitrilase-catalysed conversion ...
Streptomyces sp.
J. Biosci.
34
21-26
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712260
Thuku
Microbial nitrilases: Versatil ...
Acidovorax facilis, Acidovorax facilis 72W, Acinetobacter sp., Acinetobacter sp. AK226, Comamonas testosteroni, Pseudomonas sp., Pseudomonas sp. S1, Pyrococcus abyssi, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus rhodochrous K22, Synechocystis sp.
J. Appl. Microbiol.
106
703-727
2009
1
2
-
-
3
-
-
-
-
-
14
-
-
22
-
-
-
-
-
-
-
-
29
8
7
-
-
-
7
-
-
-
-
-
-
1
2
-
-
-
3
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
29
8
7
-
-
-
7
-
-
-
-
-
-
-
-
-
712870
Sharma
-
In silico analysis of amino ac ...
Bradyrhizobium sp., Bradyrhizobium sp. ORS278, Methylibium petroleiphilum, Pseudomonas syringae pv. syringae, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus rhodochrous K22, Synechococcus elongatus
J. Proteomics Bioinform.
2
185-192
2009
-
-
-
-
-
-
-
-
-
-
6
-
-
27
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
685014
Yeom
A determinant residue of subst ...
Rhodococcus rhodochrous
Biochem. J.
415
401-407
2008
-
-
1
1
16
-
-
31
-
-
1
-
1
2
-
-
1
1
-
-
13
-
9
1
-
-
-
32
-
-
-
-
-
-
-
-
-
1
-
1
25
-
-
-
-
32
-
-
1
-
1
-
-
1
-
-
13
-
9
1
-
-
-
32
-
-
-
-
-
-
-
-
-
-
695708
Luo
Gene cloning, overexpression, ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous tg1-A6
Appl. Biochem. Biotechnol.
160
393-400
2008
-
-
1
-
-
-
-
-
-
-
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2
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-
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-
-
-
-
-
10
-
1
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-
-
1
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-
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
687941
Khandelwal
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Optimization of nitrilase prod ...
Streptomyces sp., Streptomyces sp. MTCC 7546
J. Chem. Technol. Biotechnol.
82
646-651
2007
2
-
-
-
-
-
-
-
-
-
-
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-
2
-
-
-
-
-
-
-
-
20
-
1
1
-
-
1
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
20
-
1
1
-
-
1
1
1
-
-
-
-
-
-
-
670463
Bergeron
-
Nitrilase-catalyzed desymmetri ...
Pseudomonas fluorescens
Org. Proc. Res. Dev.
10
661-665
2006
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670792
Mueller
Cloning, overexpression, and c ...
no activity in Aeropyrum pernix, no activity in Pyrococcus furiosus, no activity in Pyrococcus horikoshii, Pyrococcus abyssi, Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543
Protein Expr. Purif.
47
672-681
2006
4
-
1
-
-
-
24
2
1
-
4
-
-
6
-
-
1
-
-
-
3
-
10
2
1
1
6
-
1
1
-
-
-
1
-
4
-
1
-
-
-
-
-
24
-
2
1
-
4
-
-
-
-
1
-
-
3
-
10
2
1
1
6
-
1
1
-
1
-
-
-
-
-
-
685425
Holtze
Transformation of the herbicid ...
Pseudomonas fluorescens 11387, Pseudomonas fluorescens, Pseudomonas putida 11388, Pseudomonas putida, Rhizobium sp. 11401, Rhizobium sp.
Biodegradation
17
503-510
2006
-
-
-
-
-
-
-
-
-
-
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6
-
-
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6
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6
-
-
-
-
-
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-
-
-
-
-
-
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-
686545
Mukherjee
-
Exploring the synthetic applic ...
Synechocystis sp.
Eur. J. Org. Chem.
23
5238-5242
2006
-
-
1
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1
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1
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-
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8
-
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-
-
-
-
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1
-
-
-
-
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-
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-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670922
Hann
-
Regioselective biocatalytic hy ...
Acidovorax facilis, Acidovorax facilis 72W
Tetrahedron
60
577-581
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654298
Chauhan
Purification, cloning, sequenc ...
Acidovorax facilis, Acidovorax facilis 72W
Appl. Microbiol. Biotechnol.
61
118-122
2003
-
1
1
-
-
-
-
-
-
-
-
-
-
10
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655310
Brenner
Catalysis in the nitrilase sup ...
Arabidopsis thaliana, Rhodococcus rhodochrous
Curr. Opin. Struct. Biol.
12
775-782
2002
-
-
-
-
-
-
-
-
-
-
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2
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-
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2
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3
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-
-
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3
-
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-
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-
-
-
-
-
-
-
655149
Dias
Enzymatic degradation of nitri ...
Meyerozyma guilliermondii, Meyerozyma guilliermondii UFMG-Y65
Can. J. Microbiol.
46
525-531
2000
-
2
1
-
-
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1
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1
-
-
-
-
2
-
-
-
-
-
1
20
-
26
-
1
-
1
-
1
-
-
-
-
-
1
-
2
1
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
1
20
-
26
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
209747
Dhillon
-
Transformation of aliphatic an ...
Pseudomonas sp., Pseudomonas sp. S1
Can. J. Microbiol.
45
811-815
1999
-
-
-
-
-
-
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6
-
-
1
-
-
3
-
-
1
1
-
-
1
-
15
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
1
-
-
1
-
15
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
32507
Levy-Schil
Aliphatic nitrilase from a soi ...
Comamonas testosteroni
Gene
161
15-20
1995
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
1
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6
-
-
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-
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-
-
-
-
-
-
-
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1
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-
-
-
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-
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-
-
-
-
-
-
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
32505
Kobayashi
Primary structure of an alipha ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous K22
Biochemistry
31
9000-9007
1992
-
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1
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2
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-
-
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1
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10
-
-
1
-
-
-
-
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6
1
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-
-
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-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
32506
Kobayashi
Purification and characterizat ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous K22
J. Bacteriol.
172
4807-4815
1990
-
-
-
-
-
-
3
2
-
1
3
-
-
6
-
-
1
-
-
-
1
-
63
2
1
1
7
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
1
3
-
-
-
-
1
-
-
1
-
63
2
1
1
7
-
1
-
2
-
-
-
-
-
-
-