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Literature summary for 3.5.5.7 extracted from

  • He, Y.C.; Ma, C.L.; Xu, J.H.; Zhou, L.
    A high-throughput screening strategy for nitrile-hydrolyzing enzymes based on ferric hydroxamate spectrophotometry (2011), Appl. Microbiol. Biotechnol., 89, 817-823.
    View publication on PubMed
Search for more literature for 3.5.5.7

Organism

Organism UniProt Comment Textmining
Alcaligenes sp.
-
-
-
Alcaligenes sp. ECU0401
-
-
-
Rhodococcus erythropolis
-
-
-
Rhodococcus erythropolis CGMCC 1.2362
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 production rates with different nitrile substrates by the cell culture Alcaligenes sp.
additional information
-
 production rates with different nitrile substrates by the cell culture Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycolonitrile + 2 H2O preferred substrate Alcaligenes sp. glycolic acid + NH3
-
 ?
glycolonitrile + 2 H2O preferred substrate Rhodococcus erythropolis glycolic acid + NH3
-
 ?
glycolonitrile + 2 H2O preferred substrate Rhodococcus erythropolis CGMCC 1.2362 glycolic acid + NH3
-
 ?
glycolonitrile + 2 H2O preferred substrate Alcaligenes sp. ECU0401 glycolic acid + NH3
-
 ?
additional information the cells are also active on benzoic acid to a lesser extent compared to aliphatic nitriles Alcaligenes sp. ?
-
 ?
additional information the cells are also active on benzoic acid to a lesser extent compared to aliphatic nitriles Rhodococcus erythropolis ?
-
 ?
additional information the cells are also active on benzoic acid to a lesser extent compared to aliphatic nitriles Rhodococcus erythropolis CGMCC 1.2362 ?
-
 ?
additional information the cells are also active on benzoic acid to a lesser extent compared to aliphatic nitriles Alcaligenes sp. ECU0401 ?
-
 ?
N-butyronitrile + 2 H2O
-
 Alcaligenes sp. N-butyric acid + NH3
-
 ?
N-butyronitrile + 2 H2O
-
 Rhodococcus erythropolis N-butyric acid + NH3
-
 ?
N-butyronitrile + 2 H2O
-
 Rhodococcus erythropolis CGMCC 1.2362 N-butyric acid + NH3
-
 ?
N-butyronitrile + 2 H2O
-
 Alcaligenes sp. ECU0401 N-butyric acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
nitrile hydratase/amidase
-
 Alcaligenes sp.
nitrile hydratase/amidase
-
 Rhodococcus erythropolis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 in vivo assay Alcaligenes sp.
30
-
 in vivo assay Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 in vivo assay Alcaligenes sp.
7
-
 in vivo assay Rhodococcus erythropolis