BRENDA - Enzyme Database show
show all sequences of 3.5.5.7

Optimization of nitrilase production from a new thermophilic isolate

Khandelwal, A.K.; Nigam, V.K.; Choudhury, B.; Mohan, M.K.; Ghosh, P.; J. Chem. Technol. Biotechnol. 82, 646-651 (2007)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Benzonitrile
inducer of nitrilase production
Streptomyces sp.
additional information
there is no appreciable variation in the activity of enzyme at any concentration of glycerol
Streptomyces sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. MTCC 7546
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methylglutaronitrile + H2O
-
687941
Streptomyces sp.
2-methylglutaric acid + NH3
-
-
-
?
acetonitrile + H2O
-
687941
Streptomyces sp.
acetic acid + NH3
-
-
-
?
acrylonitrile + H2O
-
687941
Streptomyces sp.
acrylic acid + NH3
-
-
-
?
acrylonitrile + H2O
-
687941
Streptomyces sp. MTCC 7546
acrylic acid + NH3
-
-
-
?
adiponitrile + H2O
-
687941
Streptomyces sp.
adipic acid + NH3
-
-
-
?
crotonitrile + H2O
-
687941
Streptomyces sp.
crotonic acid + NH3
-
-
-
?
crotonitrile + H2O
-
687941
Streptomyces sp. MTCC 7546
crotonic acid + NH3
-
-
-
?
fumaronitrile + H2O
-
687941
Streptomyces sp.
fumaric acid + NH3
-
-
-
?
fumaronitrile + H2O
-
687941
Streptomyces sp. MTCC 7546
fumaric acid + NH3
-
-
-
?
glutaronitrile + H2O
-
687941
Streptomyces sp.
glutaric acid + NH3
-
-
-
?
hydrocinnamonitrile + H2O
-
687941
Streptomyces sp.
hydrocinnamic acid + NH3
-
-
-
?
isobutyronitrile + H2O
-
687941
Streptomyces sp.
isobutyrate + NH3
-
-
-
?
isobutyronitrile + H2O
-
687941
Streptomyces sp. MTCC 7546
isobutyrate + NH3
-
-
-
?
isovaleronitrile + H2O
-
687941
Streptomyces sp.
isovaleric acid + NH3
-
-
-
?
mandelonitrile + 2 H2O
-
687941
Streptomyces sp.
mandelic acid + NH3
-
-
-
?
additional information
isovaleronitrile, benzonitrile, and pyridine-3-carbonitrile are weak substrates
687941
Streptomyces sp.
?
-
-
-
-
additional information
isovaleronitrile, benzonitrile, and pyridine-3-carbonitrile are weak substrates
687941
Streptomyces sp. MTCC 7546
?
-
-
-
-
propionitrile + H2O
-
687941
Streptomyces sp.
propionic acid + NH3
-
-
-
?
sebaconitrile + H2O
-
687941
Streptomyces sp.
sebaconic acid + NH3
-
-
-
?
succinonitrile + H2O
-
687941
Streptomyces sp.
succinic acid + NH3
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Streptomyces sp.
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
37
50
-
Streptomyces sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Streptomyces sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.6
7.4
-
Streptomyces sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.6
7
enzymatic activity increases in the pH range 6.6 to 7.0 and then starts decreasing
Streptomyces sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Benzonitrile
inducer of nitrilase production
Streptomyces sp.
additional information
there is no appreciable variation in the activity of enzyme at any concentration of glycerol
Streptomyces sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methylglutaronitrile + H2O
-
687941
Streptomyces sp.
2-methylglutaric acid + NH3
-
-
-
?
acetonitrile + H2O
-
687941
Streptomyces sp.
acetic acid + NH3
-
-
-
?
acrylonitrile + H2O
-
687941
Streptomyces sp.
acrylic acid + NH3
-
-
-
?
acrylonitrile + H2O
-
687941
Streptomyces sp. MTCC 7546
acrylic acid + NH3
-
-
-
?
adiponitrile + H2O
-
687941
Streptomyces sp.
adipic acid + NH3
-
-
-
?
crotonitrile + H2O
-
687941
Streptomyces sp.
crotonic acid + NH3
-
-
-
?
crotonitrile + H2O
-
687941
Streptomyces sp. MTCC 7546
crotonic acid + NH3
-
-
-
?
fumaronitrile + H2O
-
687941
Streptomyces sp.
fumaric acid + NH3
-
-
-
?
fumaronitrile + H2O
-
687941
Streptomyces sp. MTCC 7546
fumaric acid + NH3
-
-
-
?
glutaronitrile + H2O
-
687941
Streptomyces sp.
glutaric acid + NH3
-
-
-
?
hydrocinnamonitrile + H2O
-
687941
Streptomyces sp.
hydrocinnamic acid + NH3
-
-
-
?
isobutyronitrile + H2O
-
687941
Streptomyces sp.
isobutyrate + NH3
-
-
-
?
isobutyronitrile + H2O
-
687941
Streptomyces sp. MTCC 7546
isobutyrate + NH3
-
-
-
?
isovaleronitrile + H2O
-
687941
Streptomyces sp.
isovaleric acid + NH3
-
-
-
?
mandelonitrile + 2 H2O
-
687941
Streptomyces sp.
mandelic acid + NH3
-
-
-
?
additional information
isovaleronitrile, benzonitrile, and pyridine-3-carbonitrile are weak substrates
687941
Streptomyces sp.
?
-
-
-
-
additional information
isovaleronitrile, benzonitrile, and pyridine-3-carbonitrile are weak substrates
687941
Streptomyces sp. MTCC 7546
?
-
-
-
-
propionitrile + H2O
-
687941
Streptomyces sp.
propionic acid + NH3
-
-
-
?
sebaconitrile + H2O
-
687941
Streptomyces sp.
sebaconic acid + NH3
-
-
-
?
succinonitrile + H2O
-
687941
Streptomyces sp.
succinic acid + NH3
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Streptomyces sp.
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
37
50
-
Streptomyces sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Streptomyces sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.6
7.4
-
Streptomyces sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.6
7
enzymatic activity increases in the pH range 6.6 to 7.0 and then starts decreasing
Streptomyces sp.
Other publictions for EC 3.5.5.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733526
Fang
Enzymatic degradation of aliph ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous BX2
Biores. Technol.
185
28-34
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733919
Yusuf
Cloning and functional charact ...
Fusarium proliferatum, Fusarium proliferatum AUF-2
Funct. Integr. Genomics
15
413-424
2015
1
-
1
-
-
-
12
1
-
6
1
-
-
2
-
-
1
-
-
-
-
-
22
1
1
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
12
-
1
-
6
1
-
-
-
-
1
-
-
-
-
22
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
734577
Zhang
Structural insights into enzym ...
Synechocystis sp.
J. Struct. Biol.
188
93-101
2014
-
-
1
1
25
-
-
18
-
-
-
-
-
1
-
-
1
-
-
-
18
-
5
-
-
-
-
18
-
-
-
-
-
-
-
-
-
1
-
1
25
-
-
-
-
18
-
-
-
-
-
-
-
1
-
-
18
-
5
-
-
-
-
18
-
-
-
-
-
-
-
-
18
18
735340
Wang
A novel nitrilase from Rhodoba ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides LHS-305
World J. Microbiol. Biotechnol.
30
245-252
2014
-
-
1
-
-
-
9
1
-
-
2
-
-
2
-
-
1
-
-
-
-
-
19
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
9
-
1
-
-
2
-
-
-
-
1
-
-
-
-
19
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
710984
Bayer
A nitrilase from a metagenomic ...
uncultured bacterium
Appl. Microbiol. Biotechnol.
89
91-98
2011
-
-
1
-
-
-
4
1
-
-
2
-
-
2
-
-
1
-
-
-
-
-
13
1
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
1
-
-
2
-
-
-
-
1
-
-
-
-
13
1
1
-
1
-
1
1
-
-
-
-
-
-
1
1
718661
He
A high-throughput screening st ...
Alcaligenes sp., Alcaligenes sp. ECU0401, Rhodococcus erythropolis, Rhodococcus erythropolis CGMCC 1.2362
Appl. Microbiol. Biotechnol.
89
817-823
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
2
-
12
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
12
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
695831
Kim
Identification and characteriz ...
Pseudomonas fluorescens, Pseudomonas fluorescens Pf-5
Appl. Microbiol. Biotechnol.
83
273-283
2009
-
-
1
-
-
-
6
7
-
-
2
-
-
5
-
-
-
-
-
-
2
-
13
1
1
-
5
7
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
6
-
7
-
-
2
-
-
-
-
-
-
-
2
-
13
1
1
-
5
7
1
-
-
1
-
-
-
-
-
-
699065
Nigam
Nitrilase-catalysed conversion ...
Streptomyces sp.
J. Biosci.
34
21-26
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712260
Thuku
Microbial nitrilases: Versatil ...
Acidovorax facilis, Acidovorax facilis 72W, Acinetobacter sp., Acinetobacter sp. AK226, Comamonas testosteroni, Pseudomonas sp., Pseudomonas sp. S1, Pyrococcus abyssi, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus rhodochrous K22, Synechocystis sp.
J. Appl. Microbiol.
106
703-727
2009
1
2
-
-
3
-
-
-
-
-
14
-
-
22
-
-
-
-
-
-
-
-
29
8
7
-
-
-
7
-
-
-
-
-
-
1
2
-
-
-
3
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
29
8
7
-
-
-
7
-
-
-
-
-
-
-
-
-
712870
Sharma
-
In silico analysis of amino ac ...
Bradyrhizobium sp., Bradyrhizobium sp. ORS278, Methylibium petroleiphilum, Pseudomonas syringae pv. syringae, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus rhodochrous K22, Synechococcus elongatus
J. Proteomics Bioinform.
2
185-192
2009
-
-
-
-
-
-
-
-
-
-
6
-
-
27
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
685014
Yeom
A determinant residue of subst ...
Rhodococcus rhodochrous
Biochem. J.
415
401-407
2008
-
-
1
1
16
-
-
31
-
-
1
-
1
2
-
-
1
1
-
-
13
-
9
1
-
-
-
32
-
-
-
-
-
-
-
-
-
1
-
1
25
-
-
-
-
32
-
-
1
-
1
-
-
1
-
-
13
-
9
1
-
-
-
32
-
-
-
-
-
-
-
-
-
-
695708
Luo
Gene cloning, overexpression, ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous tg1-A6
Appl. Biochem. Biotechnol.
160
393-400
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
687941
Khandelwal
-
Optimization of nitrilase prod ...
Streptomyces sp., Streptomyces sp. MTCC 7546
J. Chem. Technol. Biotechnol.
82
646-651
2007
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
20
-
1
1
-
-
1
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
20
-
1
1
-
-
1
1
1
-
-
-
-
-
-
-
670463
Bergeron
-
Nitrilase-catalyzed desymmetri ...
Pseudomonas fluorescens
Org. Proc. Res. Dev.
10
661-665
2006
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670792
Mueller
Cloning, overexpression, and c ...
no activity in Aeropyrum pernix, no activity in Pyrococcus furiosus, no activity in Pyrococcus horikoshii, Pyrococcus abyssi, Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543
Protein Expr. Purif.
47
672-681
2006
4
-
1
-
-
-
24
2
1
-
4
-
-
6
-
-
1
-
-
-
3
-
10
2
1
1
6
-
1
1
-
-
-
1
-
4
-
1
-
-
-
-
-
24
-
2
1
-
4
-
-
-
-
1
-
-
3
-
10
2
1
1
6
-
1
1
-
1
-
-
-
-
-
-
685425
Holtze
Transformation of the herbicid ...
Pseudomonas fluorescens 11387, Pseudomonas fluorescens, Pseudomonas putida 11388, Pseudomonas putida, Rhizobium sp. 11401, Rhizobium sp.
Biodegradation
17
503-510
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
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