BRENDA - Enzyme Database show
show all sequences of 3.5.5.7

A determinant residue of substrate specificity in nitrilase from Rhodococcus rhodochrous ATCC 33278 for aliphatic and aromatic nitriles

Yeom, S.J.; Kim, H.J.; Lee, J.K.; Kim, D.E.; Oh, D.K.; Biochem. J. 415, 401-407 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli strain ER2566
Rhodococcus rhodochrous
Crystallization (Commentary)
Crystallization
Organism
molecular modeling of enzyme and docking studies using aromatic and aliphatic nitrile substrates
Rhodococcus rhodochrous
Engineering
Amino acid exchange
Commentary
Organism
K9A
activity similar to wild-type; increased specific activities
Rhodococcus rhodochrous
L79A
increased specific activities; slight decrease in activity towards aromatic nitriles
Rhodococcus rhodochrous
additional information
mutants containing charged amino acids such as aspartate, glutamate, arginine and asparagine at position 142 display no activity towards any nitrile, possibly owing to the disruption of hydrophobic interactions with substrates
Rhodococcus rhodochrous
R129A
inactive; loss of enzymatic activiy towards all nitriles
Rhodococcus rhodochrous
Y142A
loss of activity towards aliphatic nitriles; mutant exhibits slightly higher kcat/Km values for aromatic nitriles and shows no activity toward aliphatic nitriles; mutant specific for aromatic nitrile substrates
Rhodococcus rhodochrous
Y142D
complete loss of activity
Rhodococcus rhodochrous
Y142E
complete loss of activity
Rhodococcus rhodochrous
Y142F
mutant shows slightly lower kcat/Km values compared to the wild type enzyme; substrate specificity similar to wild-type
Rhodococcus rhodochrous
Y142G
complete loss of activity
Rhodococcus rhodochrous
Y142I
low specific activities; very low activity
Rhodococcus rhodochrous
Y142L
low specific activities; mutant specific for aromatic nitrile substrates
Rhodococcus rhodochrous
Y142N
complete loss of activity
Rhodococcus rhodochrous
Y142R
complete loss of activity
Rhodococcus rhodochrous
Y142S
mutant exhibits slightly higher kcat/Km values for aromatic nitriles and shows no activity toward aliphatic nitriles; mutant specific for aromatic nitrile substrates
Rhodococcus rhodochrous
Y142V
mutant specific for aromatic nitrile substrates
Rhodococcus rhodochrous
Y175A
activity below 6 micromol/min mg; increased specific activities
Rhodococcus rhodochrous
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.47
-
m-tolunitrile
mutant Y142V, pH 7.5, 25°C
Rhodococcus rhodochrous
0.49
-
Benzonitrile
mutant Y142V, pH 7.5, 25°C
Rhodococcus rhodochrous
0.61
-
m-tolunitrile
mutant Y142A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.688
-
adiponitrile
mutant enzyme Y142F
Rhodococcus rhodochrous
0.69
-
adiponitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
0.82
-
2-Cyanopyridine
mutant Y142A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.85
-
adiponitrile
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.91
-
Benzonitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.92
-
adiponitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.93
-
adiponitrile
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
0.933
-
adiponitrile
wild type enzyme
Rhodococcus rhodochrous
1.02
-
Benzonitrile
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.07
-
Benzonitrile
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
1.15
-
glutaronitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.19
-
glutaronitrile
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.19
-
m-tolunitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
1.24
-
glutaronitrile
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
1.241
-
glutaronitrile
wild type enzyme
Rhodococcus rhodochrous
1.25
-
2-Cyanopyridine
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.49
-
m-tolunitrile
mutant Y142S, pH 7.5, 25°C
Rhodococcus rhodochrous
1.5
-
2-Cyanopyridine
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.53
-
2-Cyanopyridine
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
1.56
-
2-Cyanopyridine
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
1.56
-
glutaronitrile
mutant enzyme Y142F; mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
1.58
-
Benzonitrile
mutant Y142A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.62
-
m-tolunitrile
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.79
-
m-tolunitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.82
-
Benzonitrile
mutant Y142S, pH 7.5, 25°C
Rhodococcus rhodochrous
1.85
-
m-tolunitrile
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
3.45
-
Benzonitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
3.7
5
2-Cyanopyridine
mutant Y142S, pH 7.5, 25°C
Rhodococcus rhodochrous
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
SDS-PAGE
Rhodococcus rhodochrous
Organic Solvent Stability
Organic Solvent
Commentary
Organism
Methanol
activity assay in presence of 10% v/v
Rhodococcus rhodochrous
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus rhodochrous
Q03217
strain ATCC 33278
-
Rhodococcus rhodochrous
-
strain ATCC 33278
-
Purification (Commentary)
Commentary
Organism
HisTrap HP 9 column chromatography
Rhodococcus rhodochrous
Reaction
Reaction
Commentary
Organism
R-CN + 2 H2O = R-COOH + NH3
hydrolysis of substrates probably involves the conjugated pi-electron system of the aromatic ring of substrate or Tyr-142 as an electron acceptor
Rhodococcus rhodochrous
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4
-
mutant enzyme Y175A, using succinonitrile as a substrate
Rhodococcus rhodochrous
1.7
-
wild type enzyme, using sebacconitrile as a substrate
Rhodococcus rhodochrous
1.8
-
mutant enzyme K9A, using sebacconitrile as a substrate
Rhodococcus rhodochrous
3.9
-
mutant enzyme Y175A, using glutaronitrile as a substrate
Rhodococcus rhodochrous
4.3
-
wild type enzyme, using succinonitrile as a substrate
Rhodococcus rhodochrous
4.8
-
mutant enzyme L79A, using sebacconitrile as a substrate
Rhodococcus rhodochrous
5.7
-
mutant enzyme Y175A, using adiponitrile as a substrate
Rhodococcus rhodochrous
16.1
-
wild type enzyme, using glutaronitrile as a substrate
Rhodococcus rhodochrous
17.7
-
mutant enzyme K9A, using glutaronitrile as a substrate
Rhodococcus rhodochrous
22.7
-
mutant enzyme L79A, using glutaronitrile as a substrate
Rhodococcus rhodochrous
29.2
-
mutant enzyme L79A, using adiponitrile as a substrate
Rhodococcus rhodochrous
31.4
-
wild type enzyme, using adiponitrile as a substrate
Rhodococcus rhodochrous
40.8
-
mutant enzyme K9A, using adiponitrile as a substrate
Rhodococcus rhodochrous
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-cyanopyridine + H2O
-
685014
Rhodococcus rhodochrous
2-pyridine carboxylic acid + NH3
-
-
-
?
adiponitrile + H2O
-
685014
Rhodococcus rhodochrous
adipic acid + NH3
-
-
-
?
adiponitrile + H2O
-
685014
Rhodococcus rhodochrous
adipate + NH3
-
-
-
?
benzonitrile + H2O
-
685014
Rhodococcus rhodochrous
benzoic acid + NH3
-
-
-
?
glutaronitrile + H2O
-
685014
Rhodococcus rhodochrous
glutarate + NH3
-
-
-
?
glutaronitrile + H2O
-
685014
Rhodococcus rhodochrous
glutaric acid + NH3
-
-
-
?
m-tolunitrile + H2O
-
685014
Rhodococcus rhodochrous
m-methylbenzoate + NH3
-
-
-
?
sebaconitrile + H2O
-
685014
Rhodococcus rhodochrous
? + NH3
-
-
-
?
succinonitrile + H2O
-
685014
Rhodococcus rhodochrous
succinate + NH3
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 40000, SDS-PAGE
Rhodococcus rhodochrous
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0001
-
m-tolunitrile
mutant Y142V, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00012
-
2-Cyanopyridine
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00014
-
Benzonitrile
mutant Y142V, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00014
-
m-tolunitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00015
-
Benzonitrile
mutant K9A, pH 7.5, 25°C; wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00017
-
m-tolunitrile
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.000175
-
2-Cyanopyridine
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00018
-
adiponitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00018
-
glutaronitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00019
-
2-Cyanopyridine
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00019
-
m-tolunitrile
mutant Y142A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.0002
-
Benzonitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00024
-
m-tolunitrile
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00025
-
2-Cyanopyridine
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00025
-
Benzonitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00028
-
m-tolunitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00029
-
Benzonitrile
mutant Y142A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00029
-
glutaronitrile
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
0.0003
-
2-Cyanopyridine
mutant Y142A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00031
-
2-Cyanopyridine
mutant Y142A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.000315
-
glutaronitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00033
-
glutaronitrile
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00038
-
m-tolunitrile
mutant Y142S, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00043
-
Benzonitrile
mutant Y142S, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00046
-
adiponitrile
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
0.0005
-
adiponitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00057
-
adiponitrile
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00073
-
2-Cyanopyridine
mutant Y142S, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00147
-
adiponitrile
mutant enzyme Y142F
Rhodococcus rhodochrous
0.00147
-
glutaronitrile
mutant enzyme Y142F
Rhodococcus rhodochrous
0.0038
-
adiponitrile
wild type enzyme
Rhodococcus rhodochrous
0.024
-
glutaronitrile
wild type enzyme
Rhodococcus rhodochrous
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli strain ER2566
Rhodococcus rhodochrous
Crystallization (Commentary) (protein specific)
Crystallization
Organism
molecular modeling of enzyme and docking studies using aromatic and aliphatic nitrile substrates
Rhodococcus rhodochrous
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
K9A
activity similar to wild-type
Rhodococcus rhodochrous
K9A
increased specific activities
Rhodococcus rhodochrous
L79A
slight decrease in activity towards aromatic nitriles
Rhodococcus rhodochrous
L79A
increased specific activities
Rhodococcus rhodochrous
additional information
mutants containing charged amino acids such as aspartate, glutamate, arginine and asparagine at position 142 display no activity towards any nitrile, possibly owing to the disruption of hydrophobic interactions with substrates
Rhodococcus rhodochrous
R129A
loss of enzymatic activiy towards all nitriles
Rhodococcus rhodochrous
R129A
inactive
Rhodococcus rhodochrous
Y142A
loss of activity towards aliphatic nitriles; mutant specific for aromatic nitrile substrates
Rhodococcus rhodochrous
Y142A
mutant exhibits slightly higher kcat/Km values for aromatic nitriles and shows no activity toward aliphatic nitriles
Rhodococcus rhodochrous
Y142D
complete loss of activity
Rhodococcus rhodochrous
Y142E
complete loss of activity
Rhodococcus rhodochrous
Y142F
substrate specificity similar to wild-type
Rhodococcus rhodochrous
Y142F
mutant shows slightly lower kcat/Km values compared to the wild type enzyme
Rhodococcus rhodochrous
Y142G
complete loss of activity
Rhodococcus rhodochrous
Y142I
very low activity
Rhodococcus rhodochrous
Y142I
low specific activities
Rhodococcus rhodochrous
Y142L
mutant specific for aromatic nitrile substrates
Rhodococcus rhodochrous
Y142L
low specific activities
Rhodococcus rhodochrous
Y142N
complete loss of activity
Rhodococcus rhodochrous
Y142R
complete loss of activity
Rhodococcus rhodochrous
Y142S
mutant specific for aromatic nitrile substrates
Rhodococcus rhodochrous
Y142S
mutant exhibits slightly higher kcat/Km values for aromatic nitriles and shows no activity toward aliphatic nitriles
Rhodococcus rhodochrous
Y142V
mutant specific for aromatic nitrile substrates
Rhodococcus rhodochrous
Y175A
activity below 6 micromol/min mg
Rhodococcus rhodochrous
Y175A
increased specific activities
Rhodococcus rhodochrous
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.47
-
m-tolunitrile
mutant Y142V, pH 7.5, 25°C
Rhodococcus rhodochrous
0.49
-
Benzonitrile
mutant Y142V, pH 7.5, 25°C
Rhodococcus rhodochrous
0.61
-
m-tolunitrile
mutant Y142A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.688
-
adiponitrile
mutant enzyme Y142F
Rhodococcus rhodochrous
0.69
-
adiponitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
0.82
-
2-Cyanopyridine
mutant Y142A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.85
-
adiponitrile
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.91
-
Benzonitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.92
-
adiponitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.93
-
adiponitrile
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
0.933
-
adiponitrile
wild type enzyme
Rhodococcus rhodochrous
1.02
-
Benzonitrile
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.07
-
Benzonitrile
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
1.15
-
glutaronitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.19
-
glutaronitrile
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.19
-
m-tolunitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
1.24
-
glutaronitrile
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
1.241
-
glutaronitrile
wild type enzyme
Rhodococcus rhodochrous
1.25
-
2-Cyanopyridine
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.49
-
m-tolunitrile
mutant Y142S, pH 7.5, 25°C
Rhodococcus rhodochrous
1.5
-
2-Cyanopyridine
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.53
-
2-Cyanopyridine
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
1.56
-
2-Cyanopyridine
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
1.56
-
glutaronitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
1.56
-
glutaronitrile
mutant enzyme Y142F
Rhodococcus rhodochrous
1.58
-
Benzonitrile
mutant Y142A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.62
-
m-tolunitrile
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.79
-
m-tolunitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
1.82
-
Benzonitrile
mutant Y142S, pH 7.5, 25°C
Rhodococcus rhodochrous
1.85
-
m-tolunitrile
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
3.45
-
Benzonitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
3.7
5
2-Cyanopyridine
mutant Y142S, pH 7.5, 25°C
Rhodococcus rhodochrous
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
SDS-PAGE
Rhodococcus rhodochrous
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
Methanol
activity assay in presence of 10% v/v
Rhodococcus rhodochrous
Purification (Commentary) (protein specific)
Commentary
Organism
HisTrap HP 9 column chromatography
Rhodococcus rhodochrous
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4
-
mutant enzyme Y175A, using succinonitrile as a substrate
Rhodococcus rhodochrous
1.7
-
wild type enzyme, using sebacconitrile as a substrate
Rhodococcus rhodochrous
1.8
-
mutant enzyme K9A, using sebacconitrile as a substrate
Rhodococcus rhodochrous
3.9
-
mutant enzyme Y175A, using glutaronitrile as a substrate
Rhodococcus rhodochrous
4.3
-
wild type enzyme, using succinonitrile as a substrate
Rhodococcus rhodochrous
4.8
-
mutant enzyme L79A, using sebacconitrile as a substrate
Rhodococcus rhodochrous
5.7
-
mutant enzyme Y175A, using adiponitrile as a substrate
Rhodococcus rhodochrous
16.1
-
wild type enzyme, using glutaronitrile as a substrate
Rhodococcus rhodochrous
17.7
-
mutant enzyme K9A, using glutaronitrile as a substrate
Rhodococcus rhodochrous
22.7
-
mutant enzyme L79A, using glutaronitrile as a substrate
Rhodococcus rhodochrous
29.2
-
mutant enzyme L79A, using adiponitrile as a substrate
Rhodococcus rhodochrous
31.4
-
wild type enzyme, using adiponitrile as a substrate
Rhodococcus rhodochrous
40.8
-
mutant enzyme K9A, using adiponitrile as a substrate
Rhodococcus rhodochrous
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-cyanopyridine + H2O
-
685014
Rhodococcus rhodochrous
2-pyridine carboxylic acid + NH3
-
-
-
?
adiponitrile + H2O
-
685014
Rhodococcus rhodochrous
adipic acid + NH3
-
-
-
?
adiponitrile + H2O
-
685014
Rhodococcus rhodochrous
adipate + NH3
-
-
-
?
benzonitrile + H2O
-
685014
Rhodococcus rhodochrous
benzoic acid + NH3
-
-
-
?
glutaronitrile + H2O
-
685014
Rhodococcus rhodochrous
glutarate + NH3
-
-
-
?
glutaronitrile + H2O
-
685014
Rhodococcus rhodochrous
glutaric acid + NH3
-
-
-
?
m-tolunitrile + H2O
-
685014
Rhodococcus rhodochrous
m-methylbenzoate + NH3
-
-
-
?
sebaconitrile + H2O
-
685014
Rhodococcus rhodochrous
? + NH3
-
-
-
?
succinonitrile + H2O
-
685014
Rhodococcus rhodochrous
succinate + NH3
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 40000, SDS-PAGE
Rhodococcus rhodochrous
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0001
-
m-tolunitrile
mutant Y142V, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00012
-
2-Cyanopyridine
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00014
-
Benzonitrile
mutant Y142V, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00014
-
m-tolunitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00015
-
Benzonitrile
mutant K9A, pH 7.5, 25°C; wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00017
-
m-tolunitrile
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.000175
-
2-Cyanopyridine
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00018
-
adiponitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00018
-
glutaronitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00019
-
2-Cyanopyridine
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00019
-
m-tolunitrile
mutant Y142A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.0002
-
Benzonitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00024
-
m-tolunitrile
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00025
-
2-Cyanopyridine
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00025
-
Benzonitrile
mutant Y142F, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00028
-
m-tolunitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00029
-
Benzonitrile
mutant Y142A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00029
-
glutaronitrile
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
0.0003
-
2-Cyanopyridine
mutant Y142A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00031
-
2-Cyanopyridine
mutant Y142A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.000315
-
glutaronitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00033
-
glutaronitrile
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00038
-
m-tolunitrile
mutant Y142S, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00043
-
Benzonitrile
mutant Y142S, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00046
-
adiponitrile
wild-type, pH 7.5, 25°C
Rhodococcus rhodochrous
0.0005
-
adiponitrile
mutant L79A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00057
-
adiponitrile
mutant K9A, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00073
-
2-Cyanopyridine
mutant Y142S, pH 7.5, 25°C
Rhodococcus rhodochrous
0.00147
-
adiponitrile
mutant enzyme Y142F
Rhodococcus rhodochrous
0.00147
-
glutaronitrile
mutant enzyme Y142F
Rhodococcus rhodochrous
0.0038
-
adiponitrile
wild type enzyme
Rhodococcus rhodochrous
0.024
-
glutaronitrile
wild type enzyme
Rhodococcus rhodochrous
Other publictions for EC 3.5.5.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733526
Fang
Enzymatic degradation of aliph ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous BX2
Biores. Technol.
185
28-34
2015
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-
-
-
-
-
-
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2
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8
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8
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
733919
Yusuf
Cloning and functional charact ...
Fusarium proliferatum, Fusarium proliferatum AUF-2
Funct. Integr. Genomics
15
413-424
2015
1
-
1
-
-
-
12
1
-
6
1
-
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2
-
-
1
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-
-
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22
1
1
1
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-
1
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1
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1
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-
-
-
-
12
-
1
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6
1
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1
-
-
-
-
22
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
734577
Zhang
Structural insights into enzym ...
Synechocystis sp.
J. Struct. Biol.
188
93-101
2014
-
-
1
1
25
-
-
18
-
-
-
-
-
1
-
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1
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-
-
18
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5
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18
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1
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1
25
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18
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1
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-
18
-
5
-
-
-
-
18
-
-
-
-
-
-
-
-
18
18
735340
Wang
A novel nitrilase from Rhodoba ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides LHS-305
World J. Microbiol. Biotechnol.
30
245-252
2014
-
-
1
-
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-
9
1
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2
-
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2
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1
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19
1
1
1
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1
1
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1
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-
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9
-
1
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2
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1
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-
-
-
19
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
710984
Bayer
A nitrilase from a metagenomic ...
uncultured bacterium
Appl. Microbiol. Biotechnol.
89
91-98
2011
-
-
1
-
-
-
4
1
-
-
2
-
-
2
-
-
1
-
-
-
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13
1
1
-
1
-
1
1
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-
-
-
-
-
-
1
-
-
-
-
-
4
-
1
-
-
2
-
-
-
-
1
-
-
-
-
13
1
1
-
1
-
1
1
-
-
-
-
-
-
1
1
718661
He
A high-throughput screening st ...
Alcaligenes sp., Alcaligenes sp. ECU0401, Rhodococcus erythropolis, Rhodococcus erythropolis CGMCC 1.2362
Appl. Microbiol. Biotechnol.
89
817-823
2011
-
-
-
-
-
-
-
-
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-
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5
-
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2
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12
-
2
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2
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-
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-
-
-
-
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-
-
-
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-
-
-
-
2
-
12
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
695831
Kim
Identification and characteriz ...
Pseudomonas fluorescens, Pseudomonas fluorescens Pf-5
Appl. Microbiol. Biotechnol.
83
273-283
2009
-
-
1
-
-
-
6
7
-
-
2
-
-
5
-
-
-
-
-
-
2
-
13
1
1
-
5
7
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
6
-
7
-
-
2
-
-
-
-
-
-
-
2
-
13
1
1
-
5
7
1
-
-
1
-
-
-
-
-
-
699065
Nigam
Nitrilase-catalysed conversion ...
Streptomyces sp.
J. Biosci.
34
21-26
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712260
Thuku
Microbial nitrilases: Versatil ...
Acidovorax facilis, Acidovorax facilis 72W, Acinetobacter sp., Acinetobacter sp. AK226, Comamonas testosteroni, Pseudomonas sp., Pseudomonas sp. S1, Pyrococcus abyssi, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus rhodochrous K22, Synechocystis sp.
J. Appl. Microbiol.
106
703-727
2009
1
2
-
-
3
-
-
-
-
-
14
-
-
22
-
-
-
-
-
-
-
-
29
8
7
-
-
-
7
-
-
-
-
-
-
1
2
-
-
-
3
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
29
8
7
-
-
-
7
-
-
-
-
-
-
-
-
-
712870
Sharma
-
In silico analysis of amino ac ...
Bradyrhizobium sp., Bradyrhizobium sp. ORS278, Methylibium petroleiphilum, Pseudomonas syringae pv. syringae, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus rhodochrous K22, Synechococcus elongatus
J. Proteomics Bioinform.
2
185-192
2009
-
-
-
-
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6
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-
27
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-
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-
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6
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6
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-
-
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-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
685014
Yeom
A determinant residue of subst ...
Rhodococcus rhodochrous
Biochem. J.
415
401-407
2008
-
-
1
1
16
-
-
31
-
-
1
-
1
2
-
-
1
1
-
-
13
-
9
1
-
-
-
32
-
-
-
-
-
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1
-
1
25
-
-
-
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32
-
-
1
-
1
-
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1
-
-
13
-
9
1
-
-
-
32
-
-
-
-
-
-
-
-
-
-
695708
Luo
Gene cloning, overexpression, ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous tg1-A6
Appl. Biochem. Biotechnol.
160
393-400
2008
-
-
1
-
-
-
-
-
-
-
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-
2
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-
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10
-
1
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1
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-
-
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1
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-
-
-
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-
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-
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-
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-
-
-
-
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10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
687941
Khandelwal
-
Optimization of nitrilase prod ...
Streptomyces sp., Streptomyces sp. MTCC 7546
J. Chem. Technol. Biotechnol.
82
646-651
2007
2
-
-
-
-
-
-
-
-
-
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2
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20
-
1
1
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1
1
1
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2
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-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
20
-
1
1
-
-
1
1
1
-
-
-
-
-
-
-
670463
Bergeron
-
Nitrilase-catalyzed desymmetri ...
Pseudomonas fluorescens
Org. Proc. Res. Dev.
10
661-665
2006
-
1
-
-
-
-
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-
-
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1
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1
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-
-
-
-
-
-
-
-
-
670792
Mueller
Cloning, overexpression, and c ...
no activity in Aeropyrum pernix, no activity in Pyrococcus furiosus, no activity in Pyrococcus horikoshii, Pyrococcus abyssi, Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543
Protein Expr. Purif.
47
672-681
2006
4
-
1
-
-
-
24
2
1
-
4
-
-
6
-
-
1
-
-
-
3
-
10
2
1
1
6
-
1
1
-
-
-
1
-
4
-
1
-
-
-
-
-
24
-
2
1
-
4
-
-
-
-
1
-
-
3
-
10
2
1
1
6
-
1
1
-
1
-
-
-
-
-
-
685425
Holtze
Transformation of the herbicid ...
Pseudomonas fluorescens 11387, Pseudomonas fluorescens, Pseudomonas putida 11388, Pseudomonas putida, Rhizobium sp. 11401, Rhizobium sp.
Biodegradation
17
503-510
2006
-
-
-
-
-
-
-
-
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-
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6
-
-
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-
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6
-
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-
-
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-
-
-
-
-
-
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-
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-
-
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6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686545
Mukherjee
-
Exploring the synthetic applic ...
Synechocystis sp.
Eur. J. Org. Chem.
23
5238-5242
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
1
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-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670922
Hann
-
Regioselective biocatalytic hy ...
Acidovorax facilis, Acidovorax facilis 72W
Tetrahedron
60
577-581
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654298
Chauhan
Purification, cloning, sequenc ...
Acidovorax facilis, Acidovorax facilis 72W
Appl. Microbiol. Biotechnol.
61
118-122
2003
-
1
1
-
-
-
-
-
-
-
-
-
-
10
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655310
Brenner
Catalysis in the nitrilase sup ...
Arabidopsis thaliana, Rhodococcus rhodochrous
Curr. Opin. Struct. Biol.
12
775-782
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
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-
-
-
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-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655149
Dias
Enzymatic degradation of nitri ...
Meyerozyma guilliermondii, Meyerozyma guilliermondii UFMG-Y65
Can. J. Microbiol.
46
525-531
2000
-
2
1
-
-
-
1
-
1
-
-
-
-
2
-
-
-
-
-
1
20
-
26
-
1
-
1
-
1
-
-
-
-
-
1
-
2
1
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
1
20
-
26
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
209747
Dhillon
-
Transformation of aliphatic an ...
Pseudomonas sp., Pseudomonas sp. S1
Can. J. Microbiol.
45
811-815
1999
-
-
-
-
-
-
-
6
-
-
1
-
-
3
-
-
1
1
-
-
1
-
15
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
1
-
-
1
-
15
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
32507
Levy-Schil
Aliphatic nitrilase from a soi ...
Comamonas testosteroni
Gene
161
15-20
1995
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
32505
Kobayashi
Primary structure of an alipha ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous K22
Biochemistry
31
9000-9007
1992
-
-
1
-
2
-
-
-
-
-
1
-
-
10
-
-
1
-
-
-
-
-
6
1
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32506
Kobayashi
Purification and characterizat ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous K22
J. Bacteriol.
172
4807-4815
1990
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3
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