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Literature summary for 3.5.5.7 extracted from
- Catalysis in the nitrilase superfamily (2002), Curr. Opin. Struct. Biol., 12, 775-782.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | P46011 | gene nit4, enzyme Nit4 | - |
| Rhodococcus rhodochrous | Q03217 | gene nitA | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| R-CN + 2 H2O = R-COOH + NH3 | enzyme structure and reaction mechanism | Arabidopsis thaliana | |
| R-CN + 2 H2O = R-COOH + NH3 | enzyme structure, covalent mechanism, formation of an enzyme-thioimidate intermediate rather than addition of water to the nitrile | Rhodococcus rhodochrous |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acrylonitrile + H2O | - |
Rhodococcus rhodochrous | acrylate + NH3 | - |
? |  |
| beta-cyano-L-alanine + H2O | - |
Arabidopsis thaliana | asparagine + aspartic acid | 60:40 mixture | ? | |
| additional information | lack of acide amide production | Rhodococcus rhodochrous | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cyanoalanine hydratase/nitrilase | - |
Arabidopsis thaliana |
| More | the enzyme belongs to branch 1.4 of the superfamily of nitrilases, overview | Arabidopsis thaliana |
| More | the enzyme belongs to the superfamily of nitrilases, branch 1.2 aliphatic nitrilases, overview | Rhodococcus rhodochrous |
| Nit4 | - |
Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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