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Literature summary for 3.5.5.7 extracted from

  • Dias, J.C.T.; Rezende, R.P.; Rosa, C.A.; Lachance, M.A.; Linardi, V.R.
    Enzymatic degradation of nitriles by a Candida guilliermondii UFMG-Y65 (2000), Can. J. Microbiol., 46, 525-531.
    View publication on PubMed
Search for more literature for 3.5.5.7

Application

Application Comment Organism
environmental protection Candida guilliermondii UFMG-Y65 might be useful for the bioremediation of environments contaminated with nitriles Meyerozyma guilliermondii
synthesis Candida guilliermondii UFMG-Y65 and the isolated enzyme, respectively, might be useful for the bioproduction of amides and acids Meyerozyma guilliermondii

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis Meyerozyma guilliermondii

Inhibitors

Inhibitors Comment Organism Structure
additional information minimal inhibition concentration MIC values of nitrile and amide substrates, the values lay between 150 mM and 2200 mM, overview Meyerozyma guilliermondii

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Meyerozyma guilliermondii 5622
-

Organism

Organism UniProt Comment Textmining
Meyerozyma guilliermondii
-
 of teleomorph Pichia guilliermondii NRRL Y-2075, identical sequence of domains D1 and D2; isolated from gold mine extraction circuit liquid, inducible enzyme
-
Meyerozyma guilliermondii UFMG-Y65
-
 of teleomorph Pichia guilliermondii NRRL Y-2075, identical sequence of domains D1 and D2; isolated from gold mine extraction circuit liquid, inducible enzyme
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information cells are able to grow on nitriles and the corresponding amides as sole nitrogen source at concentrations up to 2 M, cultivation on YCB-acetonitrile medium Meyerozyma guilliermondii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5
-
 crude enzyme extract, substrate 2-cyanopyridine Meyerozyma guilliermondii
2.7
-
 crude enzyme extract, substrate 4-cynanopyridine Meyerozyma guilliermondii
3.5
-
 crude enzyme extract, substrate adipamide Meyerozyma guilliermondii
3.6
-
 crude enzyme extract, substrate glutaronitrile Meyerozyma guilliermondii
3.9
-
 crude enzyme extract, substrate glutaramide Meyerozyma guilliermondii
4.6
-
 crude enzyme extract, substrate adiponitrile Meyerozyma guilliermondii
5
-
 crude enzyme extract, substrate benzamide Meyerozyma guilliermondii
5.6
-
 crude enzyme extract, substrate propionitrile Meyerozyma guilliermondii
6.8
-
 crude enzyme extract, substrate cyclopentanocaronitrile Meyerozyma guilliermondii
6.9
-
 crude enzyme extract, substrate benzonitrile Meyerozyma guilliermondii
7.2
-
 crude enzyme extract, substrate 3-cyanopyridine Meyerozyma guilliermondii
7.3
-
 crude enzyme extract, substrate butyronitrile Meyerozyma guilliermondii
8.1
-
 crude enzyme extract, substrate acetamide Meyerozyma guilliermondii
8.3
-
 crude enzyme extract, substrate succinamide Meyerozyma guilliermondii
8.9
-
 crude enzyme extract, substrate acrylamide Meyerozyma guilliermondii
9.2
-
 crude enzyme extract, substrate isobutyramide Meyerozyma guilliermondii
9.2
-
 crude enzyme extract, substrate isobutyronitrile Meyerozyma guilliermondii
9.7
-
 crude enzyme extract, substrate metacrylonitrile Meyerozyma guilliermondii
10.4
-
 crude enzyme extract, substrate acrylonitrile Meyerozyma guilliermondii
13.5
-
 crude enzyme extract, substrate acetonitrile Meyerozyma guilliermondii
15.8
-
 crude enzyme extract, substrate succinonitrile Meyerozyma guilliermondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-cyanopyridine + 2 H2O low activity Meyerozyma guilliermondii pyridine 2-carboxylic acid + NH3
-
 ?
3-cyanopyridine + 2 H2O
-
 Meyerozyma guilliermondii pyridine 3-carboxylic acid + NH3
-
 ?
4-cyanopyridine + 2 H2O low activity Meyerozyma guilliermondii pyridine 4-carboxylic acid + NH3
-
 ?
acetamide + H2O
-
 Meyerozyma guilliermondii ?
-
 ?
acetamide + H2O
-
 Meyerozyma guilliermondii UFMG-Y65 ?
-
 ?
acetonitrile + H2O
-
 Meyerozyma guilliermondii acetate + NH3
-
 ?
acetonitrile + H2O
-
 Meyerozyma guilliermondii UFMG-Y65 acetate + NH3
-
 ?
acrylamide + H2O
-
 Meyerozyma guilliermondii ?
-
 ?
acrylamide + H2O
-
 Meyerozyma guilliermondii UFMG-Y65 ?
-
 ?
acrylonitrile + H2O high activity Meyerozyma guilliermondii acrylic acid + NH3
-
 ?
acrylonitrile + H2O high activity Meyerozyma guilliermondii UFMG-Y65 acrylic acid + NH3
-
 ?
adipamide + H2O
-
 Meyerozyma guilliermondii ?
-
 ?
adiponitrile + H2O
-
 Meyerozyma guilliermondii adipate + NH3
-
 ?
benzamide + H2O
-
 Meyerozyma guilliermondii ?
-
 ?
benzonitrile + 2 H2O
-
 Meyerozyma guilliermondii benzoic acid + NH3
-
 ?
butyronitrile + H2O
-
 Meyerozyma guilliermondii butyric acid + NH3
-
 ?
cyclopentanocarbonitrile + H2O
-
 Meyerozyma guilliermondii cyclopentanocarbonate + NH3
-
 ?
glutaramide + H2O
-
 Meyerozyma guilliermondii ?
-
 ?
glutaronitrile + H2O
-
 Meyerozyma guilliermondii glutarate + NH3
-
 ?
glutaronitrile + H2O
-
 Meyerozyma guilliermondii UFMG-Y65 glutarate + NH3
-
 ?
isobutyramide + H2O
-
 Meyerozyma guilliermondii ?
-
 ?
isobutyronitrile + H2O
-
 Meyerozyma guilliermondii isobutyric acid + NH3
-
 ?
metacrylonitrile + H2O
-
 Meyerozyma guilliermondii metacrylic acid + NH3
-
 ?
propionitrile + H2O
-
 Meyerozyma guilliermondii propionic acid + NH3
-
 ?
succinamide + H2O
-
 Meyerozyma guilliermondii ?
-
 ?
succinonitrile + H2O best substrate Meyerozyma guilliermondii succinic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
More Candida guilliermondii is the teleomorph of Pichia guilliermondii NRRL Y-2075 Meyerozyma guilliermondii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30
-
 Meyerozyma guilliermondii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 inactivation above Meyerozyma guilliermondii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Meyerozyma guilliermondii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
150 2200 minimal inhibition concentration MIC values of nitrile and amide substrates, the values lay between 150 mM and 2200 mM, overview Meyerozyma guilliermondii additional information