BRENDA - Reference to 3.5.5.7; Id = 655149

environmental protection

Candida guilliermondii UFMG-Y65 might be useful for the bioremediation of environments contaminated with nitriles

Meyerozyma guilliermondii

synthesis

Candida guilliermondii UFMG-Y65 and the isolated enzyme, respectively, might be useful for the bioproduction of amides and acids

Meyerozyma guilliermondii

DNA sequence determination and analysis

Meyerozyma guilliermondii

additional information

minimal inhibition concentration MIC values of nitrile and amide substrates, the values lay between 150 mM and 2200 mM, overview

Meyerozyma guilliermondii

intracellular

-

Meyerozyma guilliermondii

5622

-

Meyerozyma guilliermondii

-

of teleomorph Pichia guilliermondii NRRL Y-2075, identical sequence of domains D1 and D2; isolated from gold mine extraction circuit liquid, inducible enzyme

-

Meyerozyma guilliermondii UFMG-Y65

-

of teleomorph Pichia guilliermondii NRRL Y-2075, identical sequence of domains D1 and D2; isolated from gold mine extraction circuit liquid, inducible enzyme

-

additional information

cells are able to grow on nitriles and the corresponding amides as sole nitrogen source at concentrations up to 2 M, cultivation on YCB-acetonitrile medium

Meyerozyma guilliermondii

-

2.5

-

crude enzyme extract, substrate 2-cyanopyridine

Meyerozyma guilliermondii

2.7

-

crude enzyme extract, substrate 4-cynanopyridine

Meyerozyma guilliermondii

3.5

-

crude enzyme extract, substrate adipamide

Meyerozyma guilliermondii

3.6

-

crude enzyme extract, substrate glutaronitrile

Meyerozyma guilliermondii

3.9

-

crude enzyme extract, substrate glutaramide

Meyerozyma guilliermondii

4.6

-

crude enzyme extract, substrate adiponitrile

Meyerozyma guilliermondii

5

-

crude enzyme extract, substrate benzamide

Meyerozyma guilliermondii

5.6

-

crude enzyme extract, substrate propionitrile

Meyerozyma guilliermondii

6.8

-

crude enzyme extract, substrate cyclopentanocaronitrile

Meyerozyma guilliermondii

6.9

-

crude enzyme extract, substrate benzonitrile

Meyerozyma guilliermondii

7.2

-

crude enzyme extract, substrate 3-cyanopyridine

Meyerozyma guilliermondii

7.3

-

crude enzyme extract, substrate butyronitrile

Meyerozyma guilliermondii

8.1

-

crude enzyme extract, substrate acetamide

Meyerozyma guilliermondii

8.3

-

crude enzyme extract, substrate succinamide

Meyerozyma guilliermondii

8.9

-

crude enzyme extract, substrate acrylamide

Meyerozyma guilliermondii

9.2

-

crude enzyme extract, substrate isobutyramide; crude enzyme extract, substrate isobutyronitrile

Meyerozyma guilliermondii

9.7

-

crude enzyme extract, substrate metacrylonitrile

Meyerozyma guilliermondii

10.4

-

crude enzyme extract, substrate acrylonitrile

Meyerozyma guilliermondii

13.5

-

crude enzyme extract, substrate acetonitrile

Meyerozyma guilliermondii

15.8

-

crude enzyme extract, substrate succinonitrile

Meyerozyma guilliermondii

2-cyanopyridine + H2O

low activity

655149

Meyerozyma guilliermondii

?

-

?

3-cyanopyridine + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

4-cyanopyridine + H2O

low activity

655149

Meyerozyma guilliermondii

?

-

?

acetamide + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

acetamide + H2O

-

655149

Meyerozyma guilliermondii UFMG-Y65

?

-

?

acetonitrile + H2O

-

655149

Meyerozyma guilliermondii

acetate + NH3

-

?

acetonitrile + H2O

-

655149

Meyerozyma guilliermondii UFMG-Y65

acetate + NH3

-

?

acrylamide + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

acrylamide + H2O

-

655149

Meyerozyma guilliermondii UFMG-Y65

?

-

?

acrylonitrile + H2O

high activity

655149

Meyerozyma guilliermondii

acrylic acid + NH3

-

?

acrylonitrile + H2O

high activity

655149

Meyerozyma guilliermondii UFMG-Y65

acrylic acid + NH3

-

?

adipamide + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

adiponitrile + H2O

-

655149

Meyerozyma guilliermondii

adipate + NH3

-

?

benzamide + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

benzonitrile + H2O

-

655149

Meyerozyma guilliermondii

benzoic acid + NH3

-

?

butyronitrile + H2O

-

655149

Meyerozyma guilliermondii

butyric acid + NH3

-

?

cyclopentanocarbonitrile + H2O

-

655149

Meyerozyma guilliermondii

cyclopentanocarbonate + NH3

-

?

glutaramide + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

glutaronitrile + H2O

-

655149

Meyerozyma guilliermondii

glutarate + NH3

-

?

glutaronitrile + H2O

-

655149

Meyerozyma guilliermondii UFMG-Y65

glutarate + NH3

-

?

isobutyramide + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

isobutyronitrile + H2O

-

655149

Meyerozyma guilliermondii

isobutyric acid + NH3

-

?

metacrylonitrile + H2O

-

655149

Meyerozyma guilliermondii

metacrylic acid + NH3

-

?

propionitrile + H2O

-

655149

Meyerozyma guilliermondii

propionic acid + NH3

-

?

succinamide + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

succinonitrile + H2O

best substrate

655149

Meyerozyma guilliermondii

succinic acid + NH3

-

?

25

30

-

Meyerozyma guilliermondii

55

-

inactivation above

Meyerozyma guilliermondii

7

8

-

Meyerozyma guilliermondii

150

2200

minimal inhibition concentration MIC values of nitrile and amide substrates, the values lay between 150 mM and 2200 mM, overview

Meyerozyma guilliermondii

additional information

environmental protection

Candida guilliermondii UFMG-Y65 might be useful for the bioremediation of environments contaminated with nitriles

Meyerozyma guilliermondii

synthesis

Candida guilliermondii UFMG-Y65 and the isolated enzyme, respectively, might be useful for the bioproduction of amides and acids

Meyerozyma guilliermondii

DNA sequence determination and analysis

Meyerozyma guilliermondii

150

2200

minimal inhibition concentration MIC values of nitrile and amide substrates, the values lay between 150 mM and 2200 mM, overview

Meyerozyma guilliermondii

additional information

minimal inhibition concentration MIC values of nitrile and amide substrates, the values lay between 150 mM and 2200 mM, overview

Meyerozyma guilliermondii

intracellular

-

Meyerozyma guilliermondii

5622

-

additional information

cells are able to grow on nitriles and the corresponding amides as sole nitrogen source at concentrations up to 2 M, cultivation on YCB-acetonitrile medium

Meyerozyma guilliermondii

-

2.5

-

crude enzyme extract, substrate 2-cyanopyridine

Meyerozyma guilliermondii

2.7

-

crude enzyme extract, substrate 4-cynanopyridine

Meyerozyma guilliermondii

3.5

-

crude enzyme extract, substrate adipamide

Meyerozyma guilliermondii

3.6

-

crude enzyme extract, substrate glutaronitrile

Meyerozyma guilliermondii

3.9

-

crude enzyme extract, substrate glutaramide

Meyerozyma guilliermondii

4.6

-

crude enzyme extract, substrate adiponitrile

Meyerozyma guilliermondii

5

-

crude enzyme extract, substrate benzamide

Meyerozyma guilliermondii

5.6

-

crude enzyme extract, substrate propionitrile

Meyerozyma guilliermondii

6.8

-

crude enzyme extract, substrate cyclopentanocaronitrile

Meyerozyma guilliermondii

6.9

-

crude enzyme extract, substrate benzonitrile

Meyerozyma guilliermondii

7.2

-

crude enzyme extract, substrate 3-cyanopyridine

Meyerozyma guilliermondii

7.3

-

crude enzyme extract, substrate butyronitrile

Meyerozyma guilliermondii

8.1

-

crude enzyme extract, substrate acetamide

Meyerozyma guilliermondii

8.3

-

crude enzyme extract, substrate succinamide

Meyerozyma guilliermondii

8.9

-

crude enzyme extract, substrate acrylamide

Meyerozyma guilliermondii

9.2

-

crude enzyme extract, substrate isobutyramide; crude enzyme extract, substrate isobutyronitrile

Meyerozyma guilliermondii

9.7

-

crude enzyme extract, substrate metacrylonitrile

Meyerozyma guilliermondii

10.4

-

crude enzyme extract, substrate acrylonitrile

Meyerozyma guilliermondii

13.5

-

crude enzyme extract, substrate acetonitrile

Meyerozyma guilliermondii

15.8

-

crude enzyme extract, substrate succinonitrile

Meyerozyma guilliermondii

2-cyanopyridine + H2O

low activity

655149

Meyerozyma guilliermondii

?

-

?

3-cyanopyridine + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

4-cyanopyridine + H2O

low activity

655149

Meyerozyma guilliermondii

?

-

?

acetamide + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

acetamide + H2O

-

655149

Meyerozyma guilliermondii UFMG-Y65

?

-

?

acetonitrile + H2O

-

655149

Meyerozyma guilliermondii

acetate + NH3

-

?

acetonitrile + H2O

-

655149

Meyerozyma guilliermondii UFMG-Y65

acetate + NH3

-

?

acrylamide + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

acrylamide + H2O

-

655149

Meyerozyma guilliermondii UFMG-Y65

?

-

?

acrylonitrile + H2O

high activity

655149

Meyerozyma guilliermondii

acrylic acid + NH3

-

?

acrylonitrile + H2O

high activity

655149

Meyerozyma guilliermondii UFMG-Y65

acrylic acid + NH3

-

?

adipamide + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

adiponitrile + H2O

-

655149

Meyerozyma guilliermondii

adipate + NH3

-

?

benzamide + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

benzonitrile + H2O

-

655149

Meyerozyma guilliermondii

benzoic acid + NH3

-

?

butyronitrile + H2O

-

655149

Meyerozyma guilliermondii

butyric acid + NH3

-

?

cyclopentanocarbonitrile + H2O

-

655149

Meyerozyma guilliermondii

cyclopentanocarbonate + NH3

-

?

glutaramide + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

glutaronitrile + H2O

-

655149

Meyerozyma guilliermondii

glutarate + NH3

-

?

glutaronitrile + H2O

-

655149

Meyerozyma guilliermondii UFMG-Y65

glutarate + NH3

-

?

isobutyramide + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

isobutyronitrile + H2O

-

655149

Meyerozyma guilliermondii

isobutyric acid + NH3

-

?

metacrylonitrile + H2O

-

655149

Meyerozyma guilliermondii

metacrylic acid + NH3

-

?

propionitrile + H2O

-

655149

Meyerozyma guilliermondii

propionic acid + NH3

-

?

succinamide + H2O

-

655149

Meyerozyma guilliermondii

?

-

?

succinonitrile + H2O

best substrate

655149

Meyerozyma guilliermondii

succinic acid + NH3

-

?

25

30

-

Meyerozyma guilliermondii

55

-

inactivation above

Meyerozyma guilliermondii

7

8

-

Meyerozyma guilliermondii

733526

Fang

Enzymatic degradation of aliph ...

Rhodococcus rhodochrous, Rhodococcus rhodochrous BX2

Biores. Technol.

185

28-34

2015

-

2

-

8

-

8

-

733919

Yusuf

Cloning and functional charact ...

Fusarium proliferatum, Fusarium proliferatum AUF-2

Funct. Integr. Genomics

15

413-424

2015

1

-

1

-

12

1

-

6

1

-

2

-

1

-

22

1

-

1

-

1

-

1

-

12

-

1

-

6

1

-

1

-

22

1

-

1

-

734577

Zhang

Structural insights into enzym ...

Synechocystis sp.

J. Struct. Biol.

188

93-101

2014

-

1

25

-

18

-

1

-

1

-

18

-

5

-

18

-

1

-

1

25

-

18

-

1

-

18

-

5

-

18

-

18

735340

Wang

A novel nitrilase from Rhodoba ...

Rhodobacter sphaeroides, Rhodobacter sphaeroides LHS-305

World J. Microbiol. Biotechnol.

30

245-252

2014

-

1

-

9

1

-

2

-

2

-

1

-

19

1

-

1

-

1

-

9

-

1

-

2

-

1

-

19

1

-

1

-

710984

Bayer

A nitrilase from a metagenomic ...

uncultured bacterium

Appl. Microbiol. Biotechnol.

89

91-98

2011

-

1

-

4

1

-

2

-

2

-

1

-

13

1

-

1

-

1

-

1

-

4

-

1

-

2

-

1

-

13

1

-

1

-

1

-

1

718661

He

A high-throughput screening st ...

Alcaligenes sp., Alcaligenes sp. ECU0401, Rhodococcus erythropolis, Rhodococcus erythropolis CGMCC 1.2362

Appl. Microbiol. Biotechnol.

89

817-823

2011

-

5

-

2

-

12

-

2

-

2

-

2

-

12

-

2

-

2

-

695831

Kim

Identification and characteriz ...

Pseudomonas fluorescens, Pseudomonas fluorescens Pf-5

Appl. Microbiol. Biotechnol.

83

273-283

2009

-

1

-

6

7

-

2

-

5

-

2

-

13

1

-

5

7

1

-

1

-

1

-

6

-

7

-

2

-

2

-

13

1

-

5

7

1

-

1

-

699065

Nigam

Nitrilase-catalysed conversion ...

Streptomyces sp.

J. Biosci.

34

21-26

2009

-

1

-

1

-

1

-

1

-

1

-

712260

Thuku

Microbial nitrilases: Versatil ...

Acidovorax facilis, Acidovorax facilis 72W, Acinetobacter sp., Acinetobacter sp. AK226, Comamonas testosteroni, Pseudomonas sp., Pseudomonas sp. S1, Pyrococcus abyssi, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus rhodochrous K22, Synechocystis sp.

J. Appl. Microbiol.

106

703-727

2009

1

2

-

3

-

14

-

22

-

29

8

7

-

7

-

1

2

-

3

-

14

-

29

8

7

-

7

-

712870

Sharma

-

In silico analysis of amino ac ...

Bradyrhizobium sp., Bradyrhizobium sp. ORS278, Methylibium petroleiphilum, Pseudomonas syringae pv. syringae, Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus rhodochrous K22, Synechococcus elongatus

J. Proteomics Bioinform.

2

185-192

2009

-

6

-

27

-

6

-

6

-

6

-

685014

Yeom

A determinant residue of subst ...

Rhodococcus rhodochrous

Biochem. J.

415

401-407

2008

-

1

16

-

31

-

1

-

1

2

-

1

-

13

-

9

1

-

32

-

1

-

1

25

-

32

-

1

-

1

-

1

-

13

-

9

1

-

32

-

695708

Luo

Gene cloning, overexpression, ...

Rhodococcus rhodochrous, Rhodococcus rhodochrous tg1-A6

Appl. Biochem. Biotechnol.

160

393-400

2008

-

1

-

2

-

10

-

1

-

1

-

1

-

10

-

1

-

1

-

687941

Khandelwal

-

Optimization of nitrilase prod ...

Streptomyces sp., Streptomyces sp. MTCC 7546

J. Chem. Technol. Biotechnol.

82

646-651

2007

2

-

2

-

20

-

1

-

1

-

2

-

20

-

1

-

1

-

670463

Bergeron

-

Nitrilase-catalyzed desymmetri ...

Pseudomonas fluorescens

Org. Proc. Res. Dev.

10

661-665

2006

-

1

-

1

-

1

-

670792

Mueller

Cloning, overexpression, and c ...

no activity in Aeropyrum pernix, no activity in Pyrococcus furiosus, no activity in Pyrococcus horikoshii, Pyrococcus abyssi, Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543

Protein Expr. Purif.

47

672-681

2006

4

-

1

-

24

2

1

-

4

-

6

-

1

-

3

-

10

2

1

6

-

1

-

1

-

4

-

1

-

24

-

2

1

-

4

-

1

-

3

-

10

2

1

6

-

1

-

1

-

685425

Holtze

Transformation of the herbicid ...

Pseudomonas fluorescens 11387, Pseudomonas fluorescens, Pseudomonas putida 11388, Pseudomonas putida, Rhizobium sp. 11401, Rhizobium sp.

Biodegradation

17

503-510

2006

-

6

-

6

-

6

-

686545

Mukherjee

-

Exploring the synthetic applic ...

Synechocystis sp.

Eur. J. Org. Chem.

23

5238-5242

2006

-

1

-

1

-

1

-

8

-

1

-

1

-

8

-

670922

Hann

-

Regioselective biocatalytic hy ...

Acidovorax facilis, Acidovorax facilis 72W

Tetrahedron

60

577-581

2004

-

8

-

2

-

2

-

654298

Chauhan

Purification, cloning, sequenc ...

Acidovorax facilis, Acidovorax facilis 72W

Appl. Microbiol. Biotechnol.

61

118-122

2003

-

1

-

10

-

1

-

2

-

1

-

1

-

2

-

655310

Brenner

Catalysis in the nitrilase sup ...

Arabidopsis thaliana, Rhodococcus rhodochrous

Curr. Opin. Struct. Biol.

12

775-782

2002

-

2

-

2

-

3

-

3

-

655149

Dias

Enzymatic degradation of nitri ...

Meyerozyma guilliermondii, Meyerozyma guilliermondii UFMG-Y65

Can. J. Microbiol.

46

525-531

2000

-

2

1

-

1

-

1

-

2

-

1

20

-

26

-

1

-

1

-

1

-

1

-

2

1

-

1

-

1

-

1

20

-

26

-

1

-

1

-

1

-

209747

Dhillon

-

Transformation of aliphatic an ...

Pseudomonas sp., Pseudomonas sp. S1

Can. J. Microbiol.

45

811-815

1999

-

6

-

1

-

3

-

1

-

1

-

15

1

-

1

-

6

-

1

-

1

-

1

-

15

1

-

1

-

32507

Levy-Schil

Aliphatic nitrilase from a soi ...

Comamonas testosteroni

Gene

161

15-20

1995

-

1

-

5

-

1

-

1

-

6

-

1

-

1

-

1

-

6

-

32505

Kobayashi

Primary structure of an alipha ...

Rhodococcus rhodochrous, Rhodococcus rhodochrous K22

Biochemistry

31

9000-9007

1992

-

1

-

2

-

1

-

10

-

1

-

6

1

-

1

-

2

-

1

-

1

-

6

1

-

32506

Kobayashi

Purification and characterizat ...

Rhodococcus rhodochrous, Rhodococcus rhodochrous K22

J. Bacteriol.

172

4807-4815

1990

-

3

2

-

1

3

-

6

-

1

-

1

-

63

2

1

7

-

1

-

2

-

3

-

2

-

1

3

-

1

-

1

-

63

2

1

7

-

1

-

2

-

Enzymatic degradation of nitriles by a Candida guilliermondii UFMG-Y65

Data extracted from this reference: