KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Pseudomonas sp. | |
0.75 | - |
potassium cyanide | pH 7.0, 37°C, partially purified enzyme | Pseudomonas sp. | |
5.88 | - |
acrylonitrile | pH 7.0, 37°C, partially purified enzyme | Pseudomonas sp. | |
10.28 | - |
adiponitrile | pH 7.0, 37°C, partially purified enzyme | Pseudomonas sp. | |
11.26 | - |
acetonitrile | pH 7.0, 37°C, partially purified enzyme | Pseudomonas sp. | |
12.27 | - |
Benzonitrile | pH 7.0, 37°C, partially purified enzyme | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
native PAGE | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
from soil | - |
Pseudomonas sp. S1 | - |
from soil | - |
Purification (Comment) | Organism |
---|---|
3-step procedure, partially, 10.9fold | Pseudomonas sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
R-CN + 2 H2O = R-COOH + NH3 | preferentially hydrolyses aliphatic nitriles, some of which are apparently not substrates for other known nitrilases, EC 3.5.5.1. Substrates include crotonitrile, acrylnitrile and glutaronitrile | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.53 | - |
partially purified enzyme | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetonitrile + H2O | - |
Pseudomonas sp. | acetate + NH3 | - |
? | |
acetonitrile + H2O | - |
Pseudomonas sp. S1 | acetate + NH3 | - |
? | |
acrylonitrile + H2O | most effective substrate | Pseudomonas sp. | acrylate + NH3 | - |
? | |
adiponitrile + H2O | - |
Pseudomonas sp. | adipate + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Pseudomonas sp. | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Pseudomonas sp. S1 | benzoic acid + NH3 | - |
? | |
butyronitrile + H2O | - |
Pseudomonas sp. | butyrate + NH3 | - |
? | |
butyronitrile + H2O | - |
Pseudomonas sp. S1 | butyrate + NH3 | - |
? | |
glutaronitrile + H2O | - |
Pseudomonas sp. | glutarate + NH3 | - |
? | |
glutaronitrile + H2O | - |
Pseudomonas sp. S1 | glutarate + NH3 | - |
? | |
phenylacetonitrile + 2 H2O | - |
Pseudomonas sp. | phenylacetic acid + NH3 | - |
? | |
phenylacetonitrile + 2 H2O | - |
Pseudomonas sp. S1 | phenylacetic acid + NH3 | - |
? | |
potassium cyanide + H2O | - |
Pseudomonas sp. | ? | - |
? | |
R-CN + H2O | enzyme uses both aliphatic and aromatic nitriles, no formation of amide intermediate | Pseudomonas sp. | R-COOH + NH3 | - |
? | |
succinodinitrile + H2O | - |
Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
nitrilase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas sp. |