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Literature summary for 3.5.5.5 extracted from

  • Vesela, A.; Krenkova, A.; Martinkova, L.
    Exploring the potential of fungal arylacetonitrilases in mandelic acid synthesis (2015), Mol. Biotechnol., 57, 466-474 .
No PubMed abstract available

Application

Application Comment Organism
synthesis the enzyme is promising for the enantioretentive transformation of (S)-mandelonitrile Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
expression ion Escherichia coli Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-mandelonitrile + 2 H2O the enzyme produces up to more than 70 g/l of (R)-mandelic acid (enantiomeric excess 94.5-95.6%) in batch or fed-batch mode. Its volumetric productivities are the highest in batch mode (571 g/l*d) and its catalyst productivities in fed-batch mode (39.9 g/g of dcw) Aspergillus niger (R)-mandelic acid + NH3
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Synonyms

Synonyms Comment Organism
NitAn
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Aspergillus niger