Any feedback?
Literature summary for 3.5.5.5 extracted from
- Expression, characterization of a novel nitrilase PpL19 from Pseudomonas psychrotolerans with S-selectivity toward mandelonitrile present in active inclusion bodies (2016), Biotechnol. Lett., 38, 455-461 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli BL21 (DE3) and formation ofd inclusion bodies that are active toward mandelonitrile and stable across a broad range of temperature and pH | Pseudomonas psychrotolerans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
SDS-PAGE | Pseudomonas psychrotolerans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas psychrotolerans | - |
- |
- |
| Pseudomonas psychrotolerans L19 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R,S)-mandelonitrile + 2 H2O | the enzyme hydrolyzes racemic mandelonitrile to (S)-mandelic acid with an enantiomeric excess value of 52.7%. No byproduct is observed | Pseudomonas psychrotolerans | (S)-mandelic acid + NH3 | - |
? |  |
| 1,2-phenylenediacetonitrile + H2O | activity is 4.2% compared to activity with mandelonitrile | Pseudomonas psychrotolerans | 1,2-phenylenediacetic acid + NH3 | - |
? | |
| 1,2-phenylenediacetonitrile + H2O | activity is 4.2% compared to activity with mandelonitrile | Pseudomonas psychrotolerans L19 | 1,2-phenylenediacetic acid + NH3 | - |
? | |
| 2-chloromandelonitrile + H2O | activity is 20% compared to activity with mandelonitrile | Pseudomonas psychrotolerans | 2-chloromandelic acid + NH3 | - |
? | |
| 2-cyanopyridine + 2 H2O | activity is 28% compared to activity with mandelonitrile | Pseudomonas psychrotolerans | pyridine 2-carboxylic acid + NH3 | - |
? | |
| 2-cyanopyridine + 2 H2O | activity is 28% compared to activity with mandelonitrile | Pseudomonas psychrotolerans L19 | pyridine 2-carboxylic acid + NH3 | - |
? | |
| 2-methylglutaronitrile + H2O | activity is 9.6% compared to activity with mandelonitrile | Pseudomonas psychrotolerans | 2-methylglutarate + NH3 | - |
? | |
| 3-cyanopyridine + H2O | activity is 29% compared to activity with mandelonitrile | Pseudomonas psychrotolerans | pyridine 3-carboxylic acid + NH3 | - |
? | |
| 3-hydroxyphenylpropionitrile + H2O | activity is 49% compared to activity with mandelonitrile | Pseudomonas psychrotolerans | 3-hydroxyphenylpropionic acid + NH3 | - |
? | |
| alpha-methylphenylacetonitrile + H2O | activity is 88% compared to activity with mandelonitrile | Pseudomonas psychrotolerans | alpha-methylphenylacetic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | activity is 64% compared to activity with mandelonitrile | Pseudomonas psychrotolerans | benzoate + NH3 | - |
? | |
| benzonitrile + 2 H2O | activity is 64% compared to activity with mandelonitrile | Pseudomonas psychrotolerans L19 | benzoate + NH3 | - |
? | |
| additional information | activity of less than 1% compared to the activity with madelonitrile: fumaronitrile, 3-hydroxyglutaronitrile, succinonitrile, 3-hydroxypropionitrile. The enzyme hydrolyzes nitriles with diverse structures. Arylacetonitriles are the optimal substrates | Pseudomonas psychrotolerans | ? | - |
- |
|
| additional information | activity of less than 1% compared to the activity with madelonitrile: fumaronitrile, 3-hydroxyglutaronitrile, succinonitrile, 3-hydroxypropionitrile. The enzyme hydrolyzes nitriles with diverse structures. Arylacetonitriles are the optimal substrates | Pseudomonas psychrotolerans L19 | ? | - |
- |
|
| phenylacetonitrile + 2 H2O | activity is 404% compared to activity with mandelonitrile | Pseudomonas psychrotolerans | phenylacetate + NH3 | - |
? | |
| phenylacetonitrile + 2 H2O | activity is 404% compared to activity with mandelonitrile | Pseudomonas psychrotolerans L19 | phenylacetate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, SDS-PAGE | Pseudomonas psychrotolerans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nitrilase PpL19 | - |
Pseudomonas psychrotolerans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Pseudomonas psychrotolerans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 60 | 25°C: about 85% of maximal activity, 60°C: about 80% of maximal activity | Pseudomonas psychrotolerans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
40 h, about 50% loss of activity | Pseudomonas psychrotolerans |
| 30 | - |
10 h, about 50% loss of activity | Pseudomonas psychrotolerans |
| 40 | - |
3 h, about 50% loss of activity | Pseudomonas psychrotolerans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas psychrotolerans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10 | pH 6.5: about 65% of maximal activity, pH 10.0: about 70% of maximal activity | Pseudomonas psychrotolerans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
35 h, about 50% loss of activity | Pseudomonas psychrotolerans |
| 8 | - |
12 h, about 50% loss of activity | Pseudomonas psychrotolerans |
| 8.6 | - |
3 h, about 50% loss of activity | Pseudomonas psychrotolerans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
