Literature summary for 3.5.5.5 extracted from

Eppinger, E.; Stolz, A.
Conversion of phenylglycinonitrile by recombinant Escherichia coli cells synthesizing variants of the arylacetonitrilase from Pseudomonas fluorescens EBC191 (2019), Appl. Microbiol. Biotechnol., 103, 6737-6746 .

Search for more literature for 3.5.5.5

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas fluorescens

Protein Variants

Protein Variants	Comment	Organism
A165F	decreased degree of amide formation compared to the wild type enzyme and forms about 3% phenylglycine amide from (R,S)-phenylglycinonitrile. In contrast to the wild-type enzyme, the variant almost exclusively forms (R)-phenylglycine. This point mutation results in an almost complete stereoinversion of the reaction	Pseudomonas fluorescens
W188K	the mutant enzyme produces almost exclusively phenylglycine amide from (R,S)-phenylglycinonitrile. In comparison to the wild type, there is an increase in the ee-value of the formed (S)-phenylglycine amide	Pseudomonas fluorescens

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	Q5EG61	-	-
Pseudomonas fluorescens EBC191	Q5EG61	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R,S)-phenylglycinonitrile + H2O	the wild-type enzyme, the previous mainly converts (R,S)-phenylglycinonitrile to (S)-phenylglycine with a low degree of enantioselectivity (ee 42%). In addition, about 8% of phenylglycine amide with a surplus of the (S)-enantiomer (ee 75%) is formed	Pseudomonas fluorescens	(S)-phenylglycine + phenylglycine amide + NH3	-	?
(R,S)-phenylglycinonitrile + H2O	the wild-type enzyme, the previous mainly converts (R,S)-phenylglycinonitrile to (S)-phenylglycine with a low degree of enantioselectivity (ee 42%). In addition, about 8% of phenylglycine amide with a surplus of the (S)-enantiomer (ee 75%) is formed	Pseudomonas fluorescens EBC191	(S)-phenylglycine + phenylglycine amide + NH3	-	?

Synonyms

Synonyms	Comment	Organism
NitA	-	Pseudomonas fluorescens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)