Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.5.5 extracted from

  • Sun, H.; Gao, W.; Fan, H.; Wang, H.; Wei, D.
    Cloning, purification and evaluation of the enzymatic properties of a novel arylacetonitrilase from Luminiphilus syltensis NOR5-1B: a potential biocatalyst for the synthesis of mandelic acid and its derivatives (2015), Biotechnol. Lett., 37, 1655-1661.
    View publication on PubMed
Search for more literature for 3.5.5.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Luminiphilus syltensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 2-chloromandelonitrile at pH 7.0 and 30°C Luminiphilus syltensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Luminiphilus syltensis

Organism

Organism UniProt Comment Textmining
Luminiphilus syltensis
-
-
-
Luminiphilus syltensis NOR5-1B
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Luminiphilus syltensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-bromophenyl)(hydroxy)acetonitrile + H2O 74% activity compared to phenylacetonitrile Luminiphilus syltensis (2R)-(2-bromophenyl)(hydroxy)acetic acid + NH3
-
 ?
(2-fluorophenyl)(hydroxy)acetonitrile + H2O 439% activity compared to phenylacetonitrile Luminiphilus syltensis (2R)-(2-fluorophenyl)(hydroxy)acetic acid + NH3
-
 ?
(3-bromophenyl)(hydroxy)acetonitrile + H2O 706% activity compared to phenylacetonitrile Luminiphilus syltensis (2R)-(3-bromophenyl)(hydroxy)acetic acid + NH3
-
 ?
(3-chlorophenyl)(hydroxy)acetonitrile + H2O 810% activity compared to phenylacetonitrile Luminiphilus syltensis (2R)-(3-chlorophenyl)(hydroxy)acetic acid + NH3
-
 ?
(3-fluorophenyl)(hydroxy)acetonitrile + H2O 454% activity compared to phenylacetonitrile Luminiphilus syltensis (2R)-(3-fluorophenyl)(hydroxy)acetic acid + NH3
-
 ?
(4-bromophenyl)(hydroxy)acetonitrile + H2O 680% activity compared to phenylacetonitrile Luminiphilus syltensis (2R)-(4-bromophenyl)(hydroxy)acetic acid + NH3
-
 ?
(4-chlorophenyl)(hydroxy)acetonitrile + H2O 1083% activity compared to phenylacetonitrile Luminiphilus syltensis (2R)-(4-chlorophenyl)(hydroxy)acetic acid + NH3
-
 ?
(4-fluorophenyl)(hydroxy)acetonitrile + H2O 891% activity compared to phenylacetonitrile Luminiphilus syltensis (2R)-(4-fluorophenyl)(hydroxy)acetic acid + NH3
-
 ?
1,2-phenylenediacetonitrile + H2O 1.87% activity compared to phenylacetonitrile Luminiphilus syltensis 1,2-phenylenediacetic acid + NH3
-
 ?
2-chloromandelonitrile + H2O 33.75% activity compared to phenylacetonitrile Luminiphilus syltensis (R)-2-chloromandelic acid + NH3 enantioselectivity ee 98.2% ?
2-cyanopyridine + 2 H2O 101% activity compared to phenylacetonitrile Luminiphilus syltensis pyridine 2-carboxylic acid + NH3
-
 ?
2-methylglutaronitrile + 4 H2O 0.12% activity compared to phenylacetonitrile Luminiphilus syltensis 2-methylglutaric acid + 2 NH3
-
 ?
2-phenylbutyronitrile + H2O 0.2% activity compared to phenylacetonitrile Luminiphilus syltensis 2-phenylbutyric acid + NH3
-
 ?
3-cyanopyridine + 2 H2O 43.2% activity compared to phenylacetonitrile Luminiphilus syltensis pyridine 3-carboxylic acid + NH3
-
 ?
3-hydroxyglutaronitrile + 4 H2O 0.12% activity compared to phenylacetonitrile Luminiphilus syltensis 3-hydroxyglutaric acid + 2 NH3
-
 ?
3-hydroxyphenylpropionitrile + H2O 0.12% activity compared to phenylacetonitrile Luminiphilus syltensis 3-hydroxyphenylpropionic acid + NH3
-
 ?
3-hydroxypropionitrile + H2O 0.07% activity compared to phenylacetonitrile Luminiphilus syltensis 3-hydroxypropionic acid + NH3
-
 ?
4-chlorobutyronitrile + H2O 0.06% activity compared to phenylacetonitrile Luminiphilus syltensis 4-chlorobutyric acid + NH3
-
 ?
4-cyanopyridine + 2 H2O 142% activity compared to phenylacetonitrile Luminiphilus syltensis pyridine 4-carboxylic acid + NH3
-
 ?
acrylonitrile + H2O 0.05% activity compared to phenylacetonitrile Luminiphilus syltensis acrylic acid + NH3
-
 ?
acrylonitrile + H2O 0.05% activity compared to phenylacetonitrile Luminiphilus syltensis NOR5-1B acrylic acid + NH3
-
 ?
alpha-methylphenylacetonitrile + H2O 0.14% activity compared to phenylacetonitrile Luminiphilus syltensis alpha-methylphenylacetic acid + NH3
-
 ?
benzonitrile + 2 H2O 21% activity compared to phenylacetonitrile Luminiphilus syltensis benzoic acid + NH3
-
 ?
cinnamonitrile + H2O 0.07% activity compared to phenylacetonitrile Luminiphilus syltensis cinnamic acid + NH3
-
 ?
dodecanenitrile + H2O 0.04% activity compared to phenylacetonitrile Luminiphilus syltensis dodecanoic acid + NH3
-
 ?
fumaronitrile + H2O 0.72% activity compared to phenylacetonitrile Luminiphilus syltensis fumaric acid + NH3
-
 ?
fumaronitrile + H2O 0.72% activity compared to phenylacetonitrile Luminiphilus syltensis NOR5-1B fumaric acid + NH3
-
 ?
glycolonitrile + H2O 0.08% activity compared to phenylacetonitrile Luminiphilus syltensis glycolic acid + NH3
-
 ?
hydroxy(2-methoxyphenyl)acetonitrile + H2O 178% activity compared to phenylacetonitrile Luminiphilus syltensis (2R)-hydroxy(2-methoxyphenyl)acetic acid + NH3
-
 ?
hydroxy(2-methylphenyl)acetonitrile + H2O 194% activity compared to phenylacetonitrile Luminiphilus syltensis (2R)-hydroxy(2-methylphenyl)acetic acid + NH3
-
 ?
hydroxy(phenyl)acetonitrile + H2O 404% activity compared to phenylacetonitrile Luminiphilus syltensis (2R)-hydroxy(phenyl)acetic acid + NH3
-
 ?
iminodiacetonitrile + 2 H2O 0.05% activity compared to phenylacetonitrile Luminiphilus syltensis iminodiacetic acid + 2 NH3
-
 ?
iminodiacetonitrile + 2 H2O 0.05% activity compared to phenylacetonitrile Luminiphilus syltensis NOR5-1B iminodiacetic acid + 2 NH3
-
 ?
indole-3-acetonitrile + H2O 35% activity compared to phenylacetonitrile Luminiphilus syltensis indole-3-acetic acid + NH3
-
 ?
malononitrile + H2O 3.7% activity compared to phenylacetonitrile Luminiphilus syltensis malonic acid + NH3
-
 ?
malononitrile + H2O 3.7% activity compared to phenylacetonitrile Luminiphilus syltensis NOR5-1B malonic acid + NH3
-
 ?
mandelonitrile + 2 H2O best substrate, 164% activity compared to phenylacetonitrile Luminiphilus syltensis mandelic acid + NH3
-
 ?
mandelonitrile + 2 H2O best substrate, 164% activity compared to phenylacetonitrile Luminiphilus syltensis NOR5-1B mandelic acid + NH3
-
 ?
octanedinitrile + H2O 0.14% activity compared to phenylacetonitrile Luminiphilus syltensis ? + NH3
-
 ?
succinonitrile + H2O 0.12% activity compared to phenylacetonitrile Luminiphilus syltensis succinic acid + NH3
-
 ?
valeronitrile + H2O 0.08% activity compared to phenylacetonitrile Luminiphilus syltensis valeric acid + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Luminiphilus syltensis

Synonyms

Synonyms Comment Organism
arylacetonitrile-specific nitrilase
-
 Luminiphilus syltensis
GPnor51
-
 Luminiphilus syltensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Luminiphilus syltensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 more than half maximal activity at 30 and 45°C Luminiphilus syltensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme retains 50% of its initial activity after incubation at 50°C for 30 min Luminiphilus syltensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Luminiphilus syltensis