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Literature summary for 3.5.5.5 extracted from

  • Bhatia, S.; Mehta, P.; Bhatia, R.; Bhalla, T.
    Purification and characterization of arylacetonitrile-specific nitrilase of Alcaligenes sp. MTCC 10675 (2014), Biotechnol. Appl. Biochem., 61, 459-465.
    View publication on PubMed
Search for more literature for 3.5.5.5

General Stability

General Stability Organism
in citrate buffer, the activity of the enzyme increases with the increase in the pH (4.0-5.5), whereas in borate and carbonate buffers very little activity is observed Alcaligenes sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ about 50% inhibition at 1 mM Alcaligenes sp.
Cd2+ about 50% inhibition at 1 mM Alcaligenes sp.
Co2+ 77% inhibition at 1 mM Alcaligenes sp.
Cs+ about 50% inhibition at 1 mM Alcaligenes sp.
Cu2+ 87% inhibition at 1 mM Alcaligenes sp.
dithiothreitol about 50% inhibition at 1 mM Alcaligenes sp.
Hg2+ 98% inhibition at 1 mM Alcaligenes sp.
Mg2+ 91% inhibition at 1 mM Alcaligenes sp.
Mn2+ about 50% inhibition at 1 mM Alcaligenes sp.
additional information EDTA shows very little effect on arylacetonitrilase activity Alcaligenes sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13
-
 mandelonitrile at pH 6.5 and 30°C Alcaligenes sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Zn2+, Na+, and K+ show very little effect on arylacetonitrilase activity Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 8 * 60000, SDS-PAGE Alcaligenes sp.
520000
-
 gel filtration Alcaligenes sp.

Organism

Organism UniProt Comment Textmining
Alcaligenes sp. L7RY40
-
-
Alcaligenes sp. MTCC 10675 L7RY40
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephacryl S-300 gel filtration Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphenylacetonitrile + H2O
-
 Alcaligenes sp. diphenylacetic acid + NH3
-
 ?
diphenylacetonitrile + H2O
-
 Alcaligenes sp. MTCC 10675 diphenylacetic acid + NH3
-
 ?
mandelonitrile + 2 H2O
-
 Alcaligenes sp. mandelic acid + NH3
-
 ?
mandelonitrile + 2 H2O
-
 Alcaligenes sp. MTCC 10675 mandelic acid + NH3
-
 ?
additional information the purified enzyme has very little activity against heterocyclic and aliphatic nitriles like 2-cyanopyridine and 4-cyanopyridine, and no activity is recorded against aromatic nitriles like benzonitrile, 4-hydroxynitrile, and tolunitrile Alcaligenes sp. ?
-
 ?
additional information the purified enzyme has very little activity against heterocyclic and aliphatic nitriles like 2-cyanopyridine and 4-cyanopyridine, and no activity is recorded against aromatic nitriles like benzonitrile, 4-hydroxynitrile, and tolunitrile Alcaligenes sp. MTCC 10675 ?
-
 ?
phenylacetonitrile + 2 H2O best substrate Alcaligenes sp. phenylacetic acid + NH3
-
 ?
phenylacetonitrile + 2 H2O best substrate Alcaligenes sp. MTCC 10675 phenylacetic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
homooctamer 8 * 60000, SDS-PAGE Alcaligenes sp.

Synonyms

Synonyms Comment Organism
arylacetonitrile-hydrolyzing nitrilase
-
 Alcaligenes sp.
arylacetonitrile-specific nitrilase
-
 Alcaligenes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Alcaligenes sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15 55 exposure of the enzyme at 15, 25, and 35°C for 5 h causes 8%, 15%, and 28% decrease in arylacetonitrilase activity, respectively. A further increase in the temperature to 45 and 55°C results in 58% and 90% decrease in activity, respectively. The half-life of this enzyme at 15, 25, 35, 45, 50, and 55°C is 31 h, 16 h, 9 h, 4 h 30 min, 3 h 20 min, and 2 h 40 min, respectively Alcaligenes sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26
-
 mandelonitrile at pH 6.5 and 50°C Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Alcaligenes sp.