BRENDA - Enzyme Database show
show all sequences of 3.5.5.5

Autodisplay of nitrilase from Alcaligenes faecalis in E. coli yields a whole cell biocatalyst for the synthesis of enantiomerically pure (R)-mandelic Acid

Detzel, C.; Maas, R.; Jose, J.; ChemCatChem 3, 719-725 (2011)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant functional expression of the enzyme in the cell envelope of Escherichia coli, where it is accessible for externally added protease
Alcaligenes faecalis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Alcaligenes faecalis
-
subsp. faecalis
-
Alcaligenes faecalis ATCC 8750
-
subsp. faecalis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R,S)-mandelonitrile + 2 H2O
-
719297
Alcaligenes faecalis
(R)-mandelic acid + NH3
-
-
-
?
(R,S)-mandelonitrile + 2 H2O
-
719297
Alcaligenes faecalis ATCC 8750
(R)-mandelic acid + NH3
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Alcaligenes faecalis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Alcaligenes faecalis
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant functional expression of the enzyme in the cell envelope of Escherichia coli, where it is accessible for externally added protease
Alcaligenes faecalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R,S)-mandelonitrile + 2 H2O
-
719297
Alcaligenes faecalis
(R)-mandelic acid + NH3
-
-
-
?
(R,S)-mandelonitrile + 2 H2O
-
719297
Alcaligenes faecalis ATCC 8750
(R)-mandelic acid + NH3
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Alcaligenes faecalis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Alcaligenes faecalis
Other publictions for EC 3.5.5.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733193
Sosedov
Improvement of the amides form ...
Pseudomonas fluorescens, Pseudomonas fluorescens EBC191
Appl. Microbiol. Biotechnol.
99
2623-2635
2015
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1
-
1
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11
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4
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4
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733558
Sun
Cloning, purification and eval ...
Luminiphilus syltensis, Luminiphilus syltensis NOR5-1B
Biotechnol. Lett.
37
1655-1661
2015
-
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1
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1
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1
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4
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1
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41
1
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1
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41
1
1
1
1
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1
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733546
Bhatia
Purification and characterizat ...
Alcaligenes sp., Alcaligenes sp. MTCC 10675
Biotechnol. Appl. Biochem.
61
459-465
2014
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1
10
1
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1
2
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8
1
1
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1
1
1
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718648
Baum
Conversion of sterically deman ...
Pseudomonas fluorescens, Pseudomonas fluorescens EBC191
Appl. Environ. Microbiol.
78
48-57
2012
-
1
1
-
11
-
-
1
-
-
-
2
-
10
-
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14
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1
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2
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1
1
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-
11
-
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1
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2
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-
14
-
1
-
-
-
2
-
-
-
-
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-
-
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718670
Petrickova
Purification and characterizat ...
Aspergillus niger, Aspergillus niger CBS 513.88, Neurospora crassa, Neurospora crassa OR74A
Appl. Microbiol. Biotechnol.
93
1553-1561
2012
-
-
2
-
-
-
-
6
-
-
-
6
-
7
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2
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24
-
2
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8
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2
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9
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3
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9
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6
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3
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24
-
3
-
12
-
3
-
14
-
-
1
1
-
-
-
734496
Petrickova
-
Influence of point mutations n ...
Aspergillus niger, Aspergillus niger CBS 513.88, Neurospora crassa, Neurospora crassa DSM 1257
J. Mol. Catal. B
77
74-80
2012
-
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2
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4
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5
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8
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2
4
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-
719297
Detzel
-
Autodisplay of nitrilase from ...
Alcaligenes faecalis, Alcaligenes faecalis ATCC 8750
ChemCatChem
3
719-725
2011
-
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1
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4
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1
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1
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719629
Liu
Gene cloning, expression, and ...
Alcaligenes faecalis, Alcaligenes faecalis ZJUTB10
J. Agric. Food Chem.
59
11560-11570
2011
-
1
1
-
3
-
11
1
-
2
1
2
-
2
-
-
1
1
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12
2
1
1
1
-
1
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1
1
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3
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-
11
-
1
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2
1
2
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1
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12
2
1
1
1
-
1
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-
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-
1
1
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-
-
710940
Sosedov
Construction and application o ...
Pseudomonas fluorescens
Appl. Environ. Microbiol.
76
3668-3674
2010
-
-
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-
14
-
-
-
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5
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2
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14
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2
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712643
He
Biocatalytic synthesis of (R)- ...
Alcaligenes faecalis
J. Ind. Microbiol. Biotechnol.
37
741-750
2010
-
-
-
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-
-
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4
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2
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5
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1
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1
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710930
Kiziak
Identification of amino acid r ...
Pseudomonas fluorescens
Appl. Environ. Microbiol.
75
5592-5599
2009
-
-
1
-
8
-
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5
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712260
Thuku
Microbial nitrilases: Versatil ...
Alcaligenes faecalis, Alcaligenes faecalis ATCC 8750
J. Appl. Microbiol.
106
703-727
2009
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2
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4
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6
1
1
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1
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713073
Howden
Pseudomonas syringae pv. syrin ...
no activity in no activity in Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. syringae, Pseudomonas syringae pv. syringae B728a
Mol. Plant Pathol.
10
857-865
2009
-
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10
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4
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717051
Sosedov
-
Synthesis of enantiomerically ...
Pseudomonas fluorescens, Pseudomonas fluorescens EBC191
Adv. Synth. Catal.
351
1531 - 1538
2009
-
-
1
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5
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2
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1
1
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684655
Rustler
Simultaneous expression of an ...
Pseudomonas fluorescens, Pseudomonas fluorescens EBC191
Appl. Microbiol. Biotechnol.
80
87-97
2008
-
-
1
-
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13
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1
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-
2
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1
1
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687882
Zhu
A new nitrilase from Bradyrhiz ...
Bradyrhizobium japonicum, Bradyrhizobium japonicum USDA 110
J. Biotechnol.
133
327-333
2008
-
-
1
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1
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3
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24
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1
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26
1
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1
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1
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1
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3
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1
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26
1
1
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1
1
1
1
-
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-
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694107
Banerjee
enantioselective nitrilase fro ...
Pseudomonas putida, Pseudomonas putida MTCC 5110
Mol. Biotechnol.
41
35-41
2008
-
1
1
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1
2
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2
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1
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10
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2
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1
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1
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1
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1
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10
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2
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1
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1
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700670
Agerbirk
Sinapis phylogeny and evolutio ...
Sinapis alba
Phytochemistry
69
2937-2949
2008
-
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3
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701254
Rustler
Characterisation of the substr ...
Exophiala oligosperma, Exophiala oligosperma R1
Stud. Mycol.
61
165-174
2008
1
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689852
Kiziak
Influence of different carboxy ...
Pseudomonas fluorescens, Pseudomonas fluorescens EBC191
Protein Eng. Des. Sel.
20
385-396
2007
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1
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1
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667345
Banerjee
Purification and characterizat ...
Pseudomonas putida
Arch. Microbiol.
184
407-418
2006
-
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7
3
-
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2
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3
2
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1
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1
2
18
2
1
2
3
4
1
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4
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7
-
3
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2
-
3
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1
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1
2
18
2
1
2
3
4
1
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4
-
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-
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-
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-
718003
Mateo
-
Stabilisation of oxygen-labile ...
Pseudomonas fluorescens, Pseudomonas fluorescens EBC191
J. Mol. Catal. B
38
154-157
2006
-
-
-
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1
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5
1
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1
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2
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1
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1
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2
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1
1
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-
718410
Mateo
-
Synthesis of enantiomerically ...
Pseudomonas fluorescens, Pseudomonas fluorescens EBC191
Tetrahedron Asymmetry
17
320-323
2006
-
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5
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2
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1
1
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670163
Kiziak
Nitrilase from Pseudomonas flu ...
Pseudomonas fluorescens, Pseudomonas fluorescens EBC191
Microbiology
151
3639-3648
2005
-
-
1
-
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1
-
-
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1
-
-
10
-
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2
15
1
1
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1
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1
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1
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