Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.5.5 extracted from

  • Petrickova, A.; Vesela, A.B.; Kaplan, O.; Kubac, D.; Uhnakova, B.; Malandra, A.; Felsberg, J.; Rinagelova, A.; Weyrauch, P.; K?en, V.; Bezouska, K.; Martinkova, L.
    Purification and characterization of heterologously expressed nitrilases from filamentous fungi (2012), Appl. Microbiol. Biotechnol., 93, 1553-1561.
    View publication on PubMed
Search for more literature for 3.5.5.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21-Gold (DE3) Neurospora crassa
expression in Escherichia coli strain BL21-Gold (DE3) Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 phenylpropionitrile pH 8.0, 30°C, recombinant enzyme Aspergillus niger
1.3
-
 phenylpropionitrile pH 8.0, 30°C, recombinant enzyme Neurospora crassa
2
-
 phenylacetonitrile pH 8.0, 30°C, recombinant enzyme Neurospora crassa
3.4
-
 phenylacetonitrile pH 8.0, 30°C, recombinant enzyme Aspergillus niger
3.4
-
 2-hydroxy-2-phenylacetonitrile pH 8.0, 30°C, recombinant enzyme Neurospora crassa
11.4
-
 2-hydroxy-2-phenylacetonitrile pH 8.0, 30°C, recombinant enzyme Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenylacetonitrile + 2 H2O Neurospora crassa
-
 phenylacetate + NH3
-
 ?
phenylacetonitrile + 2 H2O Aspergillus niger
-
 phenylacetate + NH3
-
 ?
phenylacetonitrile + 2 H2O Aspergillus niger CBS 513.88
-
 phenylacetate + NH3
-
 ?
phenylacetonitrile + 2 H2O Neurospora crassa OR74A
-
 phenylacetate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger A2R6M7
-
-
Aspergillus niger A9QXE0
-
-
Aspergillus niger CBS 513.88 A2R6M7
-
-
Aspergillus niger CBS 513.88 A9QXE0
-
-
Neurospora crassa
-
-
-
Neurospora crassa OR74A
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 2.4fold from Escherichia coli strain BL21-Gold(DE3) to near homogeneity Neurospora crassa
recombinant enzyme 3fold from Escherichia coli strain BL21-Gold(DE3) to near homogeneity Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-2-phenylacetonitrile + 2 H2O i.e. (R,S)-mandelonitrile Neurospora crassa 2-hydroxy-2-phenylacetate + NH3
-
 ?
2-hydroxy-2-phenylacetonitrile + 2 H2O i.e. (R,S)-mandelonitrile Aspergillus niger 2-hydroxy-2-phenylacetate + NH3
-
 ?
2-hydroxy-2-phenylacetonitrile + 2 H2O i.e. (R,S)-mandelonitrile Aspergillus niger CBS 513.88 2-hydroxy-2-phenylacetate + NH3
-
 ?
2-hydroxy-2-phenylacetonitrile + 2 H2O i.e. (R,S)-mandelonitrile Neurospora crassa OR74A 2-hydroxy-2-phenylacetate + NH3
-
 ?
phenylacetonitrile + 2 H2O
-
 Neurospora crassa phenylacetate + NH3
-
 ?
phenylacetonitrile + 2 H2O
-
 Aspergillus niger phenylacetate + NH3
-
 ?
phenylacetonitrile + 2 H2O preferred substrate Neurospora crassa phenylacetate + NH3
-
 ?
phenylacetonitrile + 2 H2O preferred substrate Aspergillus niger phenylacetate + NH3
-
 ?
phenylacetonitrile + 2 H2O
-
 Aspergillus niger CBS 513.88 phenylacetate + NH3
-
 ?
phenylacetonitrile + 2 H2O preferred substrate Aspergillus niger CBS 513.88 phenylacetate + NH3
-
 ?
phenylacetonitrile + 2 H2O
-
 Neurospora crassa OR74A phenylacetate + NH3
-
 ?
phenylacetonitrile + 2 H2O preferred substrate Neurospora crassa OR74A phenylacetate + NH3
-
 ?
phenylpropionitrile + 2 H2O
-
 Neurospora crassa phenylpropionate + NH3
-
 ?
phenylpropionitrile + 2 H2O
-
 Aspergillus niger phenylpropionate + NH3
-
 ?
phenylpropionitrile + 2 H2O
-
 Aspergillus niger CBS 513.88 phenylpropionate + NH3
-
 ?
phenylpropionitrile + 2 H2O
-
 Neurospora crassa OR74A phenylpropionate + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
 Neurospora crassa
38
-
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 purified recombinant enzyme, pH 8.0, 1 h, 90% activity remaining Neurospora crassa
25 35 purified recombinant enzyme, pH 8.0, 1 h, stable Aspergillus niger
35
-
 purified recombinant enzyme, pH 8.0, 1 h, 80% activity remaining Neurospora crassa
40
-
 purified recombinant enzyme, pH 8.0, 1 h, 40% activity remaining Neurospora crassa
40
-
 purified recombinant enzyme, pH 8.0, 1 h, 60% activity remaining Aspergillus niger
45
-
 and above, purified recombinant enzyme, pH 8.0, 1 h, no activity remaining Neurospora crassa
45
-
 purified recombinant enzyme, pH 8.0, 1 h, 20% activity remaining Aspergillus niger
50 60 purified recombinant enzyme, pH 8.0, 1 h, about 10% activity remaining Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9.5
-
 Aspergillus niger
8 8.5
-
 Neurospora crassa

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
 purified recombinant enzyme, room temperature, 2 h, 10% activity remaining Aspergillus niger
6.5 9 purified recombinant enzyme, room temperature, 2 h, 80-100% activity remaining Neurospora crassa
6.5
-
 purified recombinant enzyme, room temperature, 2 h, 50% activity remaining Aspergillus niger
7 10 purified recombinant enzyme, room temperature, 2 h, 90-100% activity remaining Aspergillus niger
9.5
-
 purified recombinant enzyme, room temperature, 2 h, 65% activity remaining Neurospora crassa
10
-
 purified recombinant enzyme, room temperature, 2 h, 20% activity remaining Neurospora crassa
10.5
-
 purified recombinant enzyme, room temperature, 2 h, 20% activity remaining Aspergillus niger
11
-
 purified recombinant enzyme, room temperature, 2 h, 10% activity remaining Neurospora crassa
11
-
 purified recombinant enzyme, room temperature, 2 h, 50% activity remaining Aspergillus niger

General Information

General Information Comment Organism
evolution the nitrilase from Neurospora crassa is related to aliphatic nitrilase, EC 3.5.5.7 Neurospora crassa