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Literature summary for 3.5.5.5 extracted from

  • Mateo, C.; Fernandes, B.; van Rantwijk, F.; Stolz, A.; Sheldon, R.A.
    Stabilisation of oxygen-labile nitrilases via co-aggregation with poly(ethyleneimine) (2006), J. Mol. Catal. B, 38, 154-157.
No PubMed abstract available
Search for more literature for 3.5.5.5

General Stability

General Stability Organism
immobilisation on poly(ethyleneimine/polyaldehyde)dextran stabilizes Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens Q5EG61
-
-
Pseudomonas fluorescens EBC191 Q5EG61
-
-

Oxidation Stability

Oxidation Stability Organism
under argon stable for more than 40 h, in oxygen atmosphere 50% loss of activity after 40 h Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.25
-
 20°C, pH 7.5 Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mandelonitrile + 2 H2O
-
 Pseudomonas fluorescens mandelic acid + NH3
-
 ?
mandelonitrile + 2 H2O
-
 Pseudomonas fluorescens EBC191 mandelic acid + NH3
-
 ?

General Information

General Information Comment Organism
metabolism involved in the biosynthesis of adenosylcobalamin Pseudomonas fluorescens