Literature summary for 3.5.5.5 extracted from

Sosedov, O.; Matzer, K.; Buerger, S.; Kiziak, C.; Baum, S.; Altenbuchner, J.; Chmura, A.; van Rantwijk, F.; Stolz, A.
Synthesis of enantiomerically pure (S)-mandelic acid using anoxynitrilase�nitrilase bienzymatic cascade: a nitrilase surprisingly shows nitrile hydratase activity (2009), Adv. Synth. Catal., 351, 1531 - 1538.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli JM 109, simultanuously with cassava (Manihot esculenta) oxynitrilase	Pseudomonas fluorescens

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	Q5EG61	-	-
Pseudomonas fluorescens EBC191	Q5EG61	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-mandelonitrile + 2 H2O	-	Pseudomonas fluorescens	(S)-mandelic acid + NH3	the product is 94% enantiomeric pure (S)-isomer, the formation of ca 50% (S)-mandeloamide is observed	?
(S)-mandelonitrile + 2 H2O	-	Pseudomonas fluorescens EBC191	(S)-mandelic acid + NH3	the product is 94% enantiomeric pure (S)-isomer, the formation of ca 50% (S)-mandeloamide is observed	?

General Information

General Information	Comment	Organism
metabolism	involved in the biosynthesis of adenosylcobalamin	Pseudomonas fluorescens

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Release 2023.1 (January 2023)