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Literature summary for 3.5.5.5 extracted from

  • He, Y.C.; Zhang, Z.J.; Xu, J.H.; Liu, Y.Y.
    Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by cetyltrimethylammonium bromide-permeabilized cells of Alcaligenes faecalis ECU0401 (2010), J. Ind. Microbiol. Biotechnol., 37, 741-750.
    View publication on PubMed
Search for more literature for 3.5.5.5

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-chloromandelonitrile + H2O Alcaligenes faecalis
-
 ?
-
 ?
3,4-dimethoxyphenylacetonitrile + H2O Alcaligenes faecalis
-
 ?
-
 ?
mandelonitrile + 2 H2O Alcaligenes faecalis
-
 mandelic acid + NH3
-
 ?
phenylacetonitrile + H2O Alcaligenes faecalis
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
 strain ECU0401
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-mandelonitrile + 2 H2O
-
 Alcaligenes faecalis (R)-(-)-mandelic acid + NH3
-
 ?
2-chloromandelonitrile + H2O
-
 Alcaligenes faecalis ?
-
 ?
3,4-dimethoxyphenylacetonitrile + H2O
-
 Alcaligenes faecalis ?
-
 ?
mandelonitrile + 2 H2O
-
 Alcaligenes faecalis mandelic acid + NH3
-
 ?
phenylacetonitrile + H2O
-
 Alcaligenes faecalis ?
-
 ?

Expression

Organism Comment Expression
Alcaligenes faecalis the nitrilase activity from Alcaligenes faecalis ECU0401 increases 4.5fold when the cells are permeabilized with 0.3% (w/v) cetyltrimethylammonium bromide for 20 min at 25°C and pH 6.5 up