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Literature summary for 3.5.5.5 extracted from

  • Kiziak, C.; Stolz, A.
    Identification of amino acid residues responsible for the enantioselectivity and amide formation capacity of the arylacetonitrilase from Pseudomonas fluorescens EBC191 (2009), Appl. Environ. Microbiol., 75, 5592-5599.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.5.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Pseudomonas fluorescens

Protein Variants

Protein Variants Comment Organism
A165E mutant with very low activities toward (R,S)-mandelonitrile and substrate (R,S)-2-phenylpropionitrile Pseudomonas fluorescens
A165F the mutant enzyme converts racemic mandelonitrile and (R,S)-2-phenylpropionitrile to increased amounts of the R enantiomers of the corresponding acids Pseudomonas fluorescens
A165G the mutant forms 4.3% amide and thus produces significantly more amide than the wild type enzyme with the substrate (R,S)-2-phenylpropionitrile Pseudomonas fluorescens
A165H the mutant enzyme shows a significantly increased relative activity for mandelonitrile (compared to (R,S)-2-phenylpropionitrile) Pseudomonas fluorescens
A165R mutant with very low activities toward (R,S)-mandelonitrile and substrate (R,S)-2-phenylpropionitrile Pseudomonas fluorescens
A165W the mutant enzyme converts racemic mandelonitrile and (R,S)-2-phenylpropionitrile to increased amounts of the R enantiomers of the corresponding acids Pseudomonas fluorescens
A165Y the mutant enzyme converts racemic mandelonitrile and (R,S)-2-phenylpropionitrile to increased amounts of the R enantiomers of the corresponding acids Pseudomonas fluorescens
C164A the mutant is devoid of nitrilase activity with the substrate (R,S)-2-phenylpropionitrile Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens Q5EG61 strain EBC191
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 (R,S)-2-phenylpropionitrile + 3 H2O NitP converts (R,S)-2-phenylpropionitrile to 2-phenylpropionic acid with a slight preference for the formation of the S enantiomer, the nitrilase converts only less than 1% of (R,S)-2-phenylpropionitrile to 2-phenylpropionamide Pseudomonas fluorescens 2-phenylpropionic acid + 2-phenylpropionamide + NH3
-
 ?
2 (R,S)-mandelonitrile + 2 H2O NitP shows only weak enantioselectivity for the formation of (R)-mandelic acid from racemic mandelonitrile Pseudomonas fluorescens (R)-mandelic acid + (S)-mandeloamide + NH3 the product ratio is about 4:1 ?

Synonyms

Synonyms Comment Organism
NitP
-
 Pseudomonas fluorescens