Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM109 | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the C-terminally truncated nitrilase variants demonstrate that deletions up to about 30 amino acids do not significantly change the relevant characteristics of the enzyme, in contrast, longer C-terminal deletions of 47-67 amino acids result in significant decreases in enzyme activities and only about 10% of the activity is found compared with the wild type enzyme, deletion mutants Nit(del-C-20) and Nit(del-C-32) strongly resemble the wild type enzyme, mutants Nit(del-C-47) to Nit(del-C-67) demonstrate reduced nitrilase activities against 2-phenylpropionitrile and show a significant decreased stability of the nitrilase activity compared with the wild type enzyme, deletion mutants also show an increased formation of amide in relation to the acid formation (up to almost 10% compared to 0.2% with the wild type enzyme) | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens EBC191 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
using 2-phenylpropionitrile as a substrate, at 30°C | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylpropionitrile + 2 H2O | - |
Pseudomonas fluorescens | 2-phenylpropionic acid + NH3 | (R)-2-phenylpropionic acid is preferentially formed | ? | |
2-phenylpropionitrile + 2 H2O | - |
Pseudomonas fluorescens EBC191 | 2-phenylpropionic acid + NH3 | (R)-2-phenylpropionic acid is preferentially formed | ? | |
mandelonitrile + 2 H2O | - |
Pseudomonas fluorescens | mandelic acid + NH3 | slight preference for the formation of (R)-mandelic acid | ? | |
mandelonitrile + 2 H2O | - |
Pseudomonas fluorescens EBC191 | mandelic acid + NH3 | slight preference for the formation of (R)-mandelic acid | ? |