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Literature summary for 3.5.5.5 extracted from
- Simultaneous expression of an arylacetonitrilase from Pseudomonas fluorescens and a (S)-oxynitrilase from Manihot esculenta in Pichia pastoris for the synthesis of (S)-mandelic acid (2008), Appl. Microbiol. Biotechnol., 80, 87-97.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas fluorescens EBC191 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-mandelonitrile + 2 H2O | - |
Pseudomonas fluorescens | (S)-mandelic acid + NH3 | - |
? |  |
| (S)-mandelonitrile + 2 H2O | - |
Pseudomonas fluorescens EBC191 | (S)-mandelic acid + NH3 | - |
? | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | - |
Pseudomonas fluorescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
no activity at pH 2 | Pseudomonas fluorescens |
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Release 2023.1 (January 2023)
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